Manual

6 Total and viability count
28
6.3 Determination of Viability
When both the concentration of non-viable cells and the total concentration of cells are
known, it is possible to calculate the viability (%viability) of the cells or the
concentration of viable cells. For the calculation the multiplication factors must be taken
into account, which is the total dilution of the original sample, as in the equation shown
below.
100%
M
C
MCMC
%viability
tt
nvnvtt
⋅
⋅
⋅
−
⋅
=
%viability: The percentage of viable cells in the original cell suspension
C
t
: The total concentration of cells in the NucleoCassette (the displayed
result of the total cell count)
C
nv
: The concentration of non-viable cells in the NucleoCassette (the result
displayed when counting the non-viable cells)
M
t
: The multiplication factor used for the total cell count, e.g. the dilution of
the sample with diluent and lysing reagent
M
nv
: The multiplication factor used for the counting of non-viable cells, e.g.
the combined dilution of the sample by diluent