Manual

5 Operation of the NucleoCounter
25
elevated signal levels. If the number of pixels is above a predefined limit then the
cassette insertion slit can be contaminated with an interfering particle, like a dust
particle. An error message is shown in the display and a cleaning of the insertion slit is
recommended (refer to chapter 7 Maintenance of NucleoCounter).
If a PC with NucleoView is connected to the instrument a contaminant is usually
observed as an object, which is stationary in all images.
To initiate a Zero Count Check type in “F50 + ↵
↵↵
↵”. Then the following text will appear in
the display:
0 count check
0 count check 0 count check
0 count check
↵
↵↵
↵ Continue
ContinueContinue
Continue
Esc Ab
Esc AbEsc Ab
Esc Abort
ortort
ort
Figure 20 Zero Count Check, step 1
Press “↵
↵↵
↵” to continue. Now the instrument prompts you to remove any cassette from
the insertion slit:
Remove any
Remove anyRemove any
Remove any
cassette
cassettecassette
cassette
↵
↵↵
↵ Continue
ContinueContinue
Continue
Esc Abort
Esc AbortEsc Abort
Esc Abort
Figure 21 Zero Count Check, step 2
Press “↵
↵↵
↵” to continue. Now the instrument prompts you to close the lid in order to avoid
false light from entering the insertion slit during the Zero Count Check:
Close lid
Close lidClose lid
Close lid
↵
↵↵
↵ Continue
ContinueContinue
Continue
Esc Abort
Esc AbortEsc Abort
Esc Abort
Figure 22 Zero Count Check, step 3
Press “↵
↵↵
↵” to continue. Then a “Wait” message appears in the display:
wait...
wait...wait...
wait...
Figure 23 Zero Count Check, step 3A