Owner's manual

Components
Leica Angle Two
™
User Manual Rev A02 © Leica Biosystems Richmond Inc. 2010 11
Caution
Do not use the rat incisor bar on a mouse. A mouse adaptor is available from Leica Microsystems
Note: Hamsters and gerbils can use the rat ear bars and incisor bar.
Nose Clamp
The nose clamp is placed over the animal’s nose and gently lowered using the thumbscrew
indicated. The nose clamp helps to keep the animal in position when injectable anesthesia is used.
Figure 6. The nose clamp
If using gas anesthesia with a nose cone, remove the nose clamp by undoing the thumbscrew and
insert the nose cone.
Ear Bars
The ear bars are tapered at one end to fit deep inside the ear canal. The instrument comes with
two rat ear bars (18° and 45°). Other animal ear bars and adaptors can be purchased from Leica
Microsystems.
After the bars are positioned in the animal’s ears, the animal is centered in the middle of the
U frame using the scales on each bar. Use this measurement to reproduce the exact position of the
animal for repeat studies.
Nose clamp
Vertical control
Removes the nose
clamp