GelDoc-It®2 & ChemiDoc-It®2 Imagers Installation and User Instructions UVP, LLC 2066 W. 11th Street Upland, CA 91786 Phone: (800) 452-6788 Fax: (909) 946-3597 Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate Nuffield Road, Cambridge CB4 1TG UK Phone: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.
2 GelDoc-It and ChemiDoc-It Imagers 2 Table of Contents Table of Contents .........................................................................................................................................................2 Introduction ..................................................................................................................................................................3 System Components ..................................................................................
2 2 GelDoc-It and ChemiDoc-It Imagers 3 Introduction 2 The GelDoc-It Imager is designed as a high resolution imager capable of capturing, documenting and analyzing fluorescent gel images. 2 In addition to gel documentation capabilities, the ChemiDoc-It Imager is equipped with a cooled, scientificgrade CCD camera allowing users to capture, document and analyze chemiluminescent blot images, including Western blots.
2 2 GelDoc-It and ChemiDoc-It Imagers 4 System Components Refer to the packing slip and pictured components for parts included with the system.
2 2 GelDoc-It and ChemiDoc-It Imagers 5 Cameras 2 2 GelDoc-It : The GelDoc-It is equipped with the GelCam 310 camera, a scientific-grade monochrome CCD camera with a resolution of 2.0MP (1600x1200) with USB 2.0 PC interface. 2 2 ChemiDoc-It : The ChemiDoc-It is equipped with the BioChemi™ 510, OptiChemi™ 610 or MegaCam 810 scientific-grade monochrome CCD camera. All cameras are Peltier cooled and offer full 16-bit file bit depth: The BioChemi 510 camera has 2.
2 2 GelDoc-It and ChemiDoc-It Imagers 6 Transilluminator 2 2 The GelDoc-It and ChemiDoc-It Imagers can accommodate UVP’s Benchtop and FirstLight transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop models with multiple wavelengths and variable intensities as well as the highly uniform, patented ® FirstLight transilluminator. Refer to the packing slip for the transilluminator included with your system.
2 2 GelDoc-It and ChemiDoc-It Imagers 7 LED White Light Plate The LED White Light Plate emits high uniformity with less than 5% coefficient of variance (CV). Plug the LED White Light Plate directly into the power supply within the system darkroom, or store it in the exterior darkroom side pocket when not in use.
2 2 GelDoc-It and ChemiDoc-It Imagers 8 Setup Instructions Components 2 2 When unpacking the GelDoc-It and ChemiDoc-It , the following items will be included: 2 2 GelDoc-It or ChemiDoc-It darkroom Transilluminator Camera with lens and bracket Ethidium Bromide (EtBr) emission filter Electrical and computer cables VisionWorksLS Software WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on unless otherwise instructed.
2 2 GelDoc-It and ChemiDoc-It Imagers 9 Additional and replacement emission filters are available through UVP. Refer to the Replacement Parts and Accessories section of this manual for ordering information. Camera Setup 2 2 High sensitivity, scientific-grade CCD cameras are designed for use with UVP’s GelDoc-It and ChemiDoc-it Imagers. This section covers the components and steps required to install UVP’s cameras.
2 2 GelDoc-It and ChemiDoc-It Imagers 10 GelCam 310 Camera with Motorized Lens The GelCam 310 camera, zoom lens and diopter are assembled at the UVP factory. (Note: Camera may be a different size or color than shown) 1. Remove the cap from the lens (if in place). 2. Using the four brass thumb screws provided, secure the bracket to the base of the darkroom. 3. Black thumb screw Slide the camera and lens assembly into the camera bracket.
2 2 GelDoc-It and ChemiDoc-It Imagers 11 7. Plug the serial cable from the motorized lens into the lens control box. 8. Connect the USB cable to the PC connection port on the lens control box. Leave the other end of the USB cable unplugged until installation of VisionWorksLS is complete. 9. Connect the power supply to the lens control box and into the power outlet.
2 2 GelDoc-It and ChemiDoc-It Imagers 1. USB Cable 2. MegaCam 810/OptiChemi 610/BioChemi 510 Camera 3. Base Mount (ships attached to the darkroom) 4. Bracket 5. Black Thumb Screw and Brass Thumb Nuts 6. Camera Lens & Controller 7. Lens Controller Power Supply Cable 12 4 2 3 6 7 1 5 Hardware Installation The photograph shows the parts required for assembly of the MegaCam 810 and BioChemi 510 camera 2 kits with the motorized lens on the ChemiDoc-It Imager.
2 2 GelDoc-It and ChemiDoc-It Imagers Registering the Software 1. Double click the VisionWorksLS software icon on the desktop. 2. To activate the software, registration is required. To immediately activate the software online, choose On-theFly activation. If the computer is not connected to the Internet, select Offline activation and proceed to the following page of this manual, or call UVP to register the software. 3. Click Next to continue. 4.
2 2 GelDoc-It and ChemiDoc-It Imagers 14 Installing GelCam 310 Camera Drivers 1. Open the VisionWorksLS software suite. 2. Plug the 90 degree USB cable from the camera into the USB port. 3. The computer should display Found New Hardware. Click Yes, Next, Install from a list, Next, Don’t search, Next, double click on Show all Devices, click Have disk, and then Browse. 4. Browse to the C:\Windows\system32\drivers folder and select the STUSB.inf file. 5.
2 2 GelDoc-It and ChemiDoc-It Imagers 15 Using the System Powering Up the System 2 2 Once plugged in to a surge-protected wall outlet, the GelDoc-It and ChemiDoc-It systems are always powered on. Power to specific system components, including epi and transillumination lighting, is controlled by switches located on the front of the unit. 2 If using the GelDoc-It system with the GelCam 310 camera, turn on the power switch for the Lens Control Box (see “Camera Setup” for more information).
2 2 GelDoc-It and ChemiDoc-It Imagers 16 Using the Transilluminator To use the UV transilluminator or LED White Light Plate, ensure that the components are plugged into the system (see “Connecting the Power Cables”). Using the power switches located on the front of the unit, select either White Light: Trans or Ultraviolet: Trans depending upon application. When using the UV transilluminator, ensure that the green power switch on the front of the transilluminator unit is in the ON position.
2 2 GelDoc-It and ChemiDoc-It Imagers 17 Capturing Images and Using Templates Image Focusing Manual Lens (GelCam 310 with Manual Lens) Prior to capturing an image, prepare the image focus: 1. Turn on the transilluminator and place the Fluorescent Focus Target (see “System Components”) on the transilluminator surface. NOTE: The darkroom has a UV safety switch that turns off the transilluminator and optional UV handheld lamps when the door is open.
2 2 GelDoc-It and ChemiDoc-It Imagers 18 Recommended Settings for Short Exposures and Focusing 1. Increasing the Binning will increase the camera’s refresh rate and sensitivity. However, it also exponentially decreases overall image resolution. Binning works by combining smaller pixels into one larger pixel.
2 2 GelDoc-It and ChemiDoc-It Imagers 19 To Process a Blot 1. Place the Western blot on a hinged transparency or in clear plastic bag. 2. Position the blot on the black chemi tray. 3. Pre-focus and position the blot with the lights off, the door cracked open and the lens set to the fastest f-stop (the lowest aperture number). Use the “Chemi Blot Pre-Capture Positioning” template discussed earlier. 4. Apply the chemi substrate on the blot (front side up).
2 2 GelDoc-It and ChemiDoc-It Imagers 20 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Replacement Parts and Accessories To order accessories (including additional emission filters) or replacement parts for the GelDoc-It System, contact UVP’s offices.
2 2 GelDoc-It and ChemiDoc-It Imagers 21 A Partial Image Appears on the Screen 1. Ensure that the filter wheel is not off center by turning the filter wheel selector until it fully engages or “clicks” into place. 2. Remove the camera/lens combination and verify that the emission filter is fully seated in the filter wheel. Transilluminator Will Not Turn On 2 2 1.
