Owner manual

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4 Installation

940 Professional IC Vario ONE/SeS/Prep 1 (2.940.1410)

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￭ Rinse the separation column with eluent for approximately 10

minutes.

￭ Use manual control in MagIC Net to stop the high-pressure pump

again: Off.

4.22 Conditioning

After installation, after each time the instrument is switched on and after

each time the eluent is changed, the system has to be conditioned with

eluent long enough that a stable baseline is attained.

NOTE

The conditioning time can lengthen considerably after changing the

eluent.

Conditioning the system

Preparing the software

CAUTION

Ensure that the configured flow is not higher than the flow permit-

ted for the corresponding column (refer to the column leaflet and

the chip data record).

￭ Start the MagIC Net computer program.

￭ Open the Equilibration tab in MagIC Net: Work-

place ▶ Run ▶ Equilibration.

￭ Select (or create) a suitable method.

Also see: MagIC Net tutorial and online help.

Preparing the instrument

￭ Ensure that the column is inserted correctly in relation to the flow

direction marked on the sticker (arrow has to point in the direc-

tion of flow).

￭ Ensure that the eluent aspiration tubing is immersed in the eluent

and there is enough eluent in the eluent bottle.

Starting equilibration

￭ Start the equilibration in MagIC Net: Workplace ▶ Run ▶ Equi-

libration ▶ Start HW.