Owner's manual

Methods
Eppendorf BioSpectrometer
®
basic
English (EN)
38
Correct A260 1 Selection:
on | off
Only for the Dye labels method group.
Correction of the influence of the dye spectrum on the
absorbance with the measuring wavelength of the biomolecule
(260 nm or 280 nm). Some of the dye spectra have a low
absorbance at 260 and 280 nm. These absorbances distort the
calculations for the nucleic acids or the proteins of these
methods. To minimize the distortion correction factors are used,
as long as these are known for the corresponding dyes. When
the parameter is switched on, the correction factor is imported
from the General Method Parameters/Dyes function.
Correct A 280 1 Selection:
on | off
Only for the Dye labels method group.
For an explanation see the description of the Correct A260 1
parameter above.
Dye 2 active Selection:
on | off
Only for the Dye labels method group.
Option to measure a second dye at the same time. Application:
Labeling a biomolecule with two dyes.
Dye 2 Selection:
List of dyes that are
stored in the General
Method Parameters/
Dyes function.
Only for the Dye labels method group when measuring 2 dyes.
Selection of the second dye (see Dye 1 parameter).
Correct A260 2 Selection:
on | off
Only for the Dye labels method group when measuring 2 dyes.
Analog to parameter Correct A260 1.
Correct A280 2 Selection:
on | off
Only for the Dye labels method group when measuring 2 dyes.
Analog to parameter Correct A280 1.
Show scan Selection:
on | off
Display of a scan (absorbance wavelength graph) in addition to
the result of the sample measurement.
Start λ Value input:
Wavelength in nm.
Range: 200 to 830 nm.
Starting wavelength for recording the scan.
Stop λ Value input:
Wavelength in nm.
Range: 200 to 830 nm.
Value must be higher
than the value for Start
λ.
Stopping wavelength for recording the scan.
A260/A280 Selection:
on | off
Only for nucleic acids.
Display of the A260/A280 ratio in addition to the result of the
sample measurement.
A260/A230 Selection:
on | off
Only for nucleic acids.
Display of the A260/A230 ratio in addition to the result of the
sample measurement.
Parameter Entry Explanation