Instruction Manual
4 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Setup and Basic 
Operation
2
2.1 Workflow Overview 
Prepare Buffers
Prepare sample and running buffers
Prepare and Load Samples 
Dilute in sample buffer
Prepare Gels and 
Assemble Electrophoresis Cell
Analyze the Separation
(Chapter 12)
Perform Electrophoresis
SDS-PAGE (Chapter 3)
Native PAGE (Chapter 4)
Peptide Analysis (Chapter 6)
Isoelectric Focusing (Chapter 7)
Protease Analysis (Chapter 8)
Nondenaturing Nucleic Acid PAGE (Chapter 9)
Denaturing Nucleic Acid PAGE (Chapter 10)
2-D Electrophoresis (Chapter 11)
Blot the Gels (Optional) 
(Chapter 13)










