MPA-10/MPA-20/MPA-200/MPA-1200 Single Channel Electronic Pipette INSTRUCTION MANUAL 1WMPD4002874B
WARNING DEFINITIONS The warnings described in this manual have the following meanings: DANGER An imminently hazardous situation which, if not avoided, will result in death or serious injury. WARNING A potentially hazardous situation which, if not avoided, could result in death or serious injury. CAUTION A potentially hazardous situation which, if not avoided, may result in minor or moderate injury or damage to the instrument. NOTE Information or cautions to use the device correctly.
CONTENTS 1. FOR SAFE USE.......................................................................................................... 3 1-1 Precautions on using the pipette ...............................................................................................3 1-2 Precautions on handling the battery..........................................................................................3 2. INTRODUCTION...................................................................................................
17. LIST OF ITEMS SOLD SEPARATELY（DISPOSABLE ITEMS）.....................39 17-1 Stands and hanger..................................................................................................................39 17-2 Tips and accessories..............................................................................................................40 17-3 Disposable items (User replaceable) ....................................................................................42 17-4 Others ....................
1. FOR SAFE USE 1-1 Precautions on using the pipette DANGER - This instrument is not an explosion proof instrument. Do not use the pipette in an environment where there is a risk of explosion, or use it for explosive chemicals that may cause explosion. - When using potentially harmful solutions, such as infectious bacteria or viruses, radioactive substances that have a risk of exposure, or poisons, exercise extreme caution and follow all safety measures.
CAUTION - Do not use a leaking battery. - Because the battery body may become hot when using the pipette continuously for a long time, take care not to get burned when handling it. - Should you get battery fluid from a leaking battery in your eye, immediately flush with copious amounts of water and seek immediate medical attention. Should the liquid contact the clothes or skin, rinse immediately with copious amounts of water. 2. INTRODUCTION Thank you for purchasing the MPA series electronic pipette.
4. COMPLIANCE Compliance with FCC Rules Please note that this device generates, uses and can radiate radio frequency energy. This device has been tested and has been found to comply with the limits of a Class A computing device pursuant to Subpart J of Part 15 of FCC rules. These rules are designed to provide reasonable protection against interference when this device is operated in a commercial environment.
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5. MPA FUNCTION □ The pipette has three modes where advantages of electromotion are utilized. (Refer to “9. FUNCTION AND HOW TO USE”) - Standard mode (AUTO) This is for basic pipette operation. In this mode, the pipette aspirates once and then dispenses once. - Multiple dispensing mode (MD) This is for dispensing liquid on a microplate, etc. In this mode, the pipette aspirates once and dispenses several times. - Mixing mode (MIX) This is a useful operation when uniformly mixing different types of liquids.
6. PACKING CONTENTS AND NAME OF ITEMS Confirm that the following contents are all included. ○ Electronic pipette MPA-10 / 20 / 200 / 1200 (Any one among them) ○ Accessories (1) Battery (1 pc) (2) The AC adapter (Combined use for charging) (Switching with AC100V to 240V) Selectable power plug (A / BF / C / S type) * AC adapter has the A type AC adapter plug attached. Use AC adapter plug for AC adapter to match local outlet.
(1) Battery (2) AC adapter * A type BF type (3) Power cable C type S type Selectable power plug (7) Pipette tip (Sample, each 1 pc) Pipette (4) Instruction manual (5) Quick operation guide For 10/20μL (For MPA-10/20) For 200μL (For MPA-200) (6) Performance certification (8) Name sticker For 1200μL (For MPA-1200) Should the pipette arrive damaged or an accessory be missing, contact the nearest A&D dealer. Note The accessories included with this product may be changed without notice.
7. PREPARATION BEFORE USE 7-1 Installing the battery 1. Remove the battery cover (2) by sliding it upward while pressing and holding the battery cover release button (1). 2. Connect the terminal of the battery’s cable, as shown in the figure below, to the connector for the battery in the bottom of the battery compartment. When connecting the terminal, be sure it is connected in the proper direction. 3. Install the battery so that the battery cable is in the cable guide. 4.
7-2 Recharging the battery When purchasing the instrument, the battery does not have a full charge. For initial use, first charge the battery fully. Recharge the battery with the battery installed in the pipette. Pipette use is available during recharging. Recharging 1. Remove the power connector cover from the pipette. 2. Connect the power cable connected to the AC adapter to the power connector on the pipette. 3. Connect the AC adapter plug to the outlet.
7-3 Exchange selectable power plug The A-Type power supply plug is originally attached to the AC adapter. Please change the power supply plug to the one that suits your location. Exchange method 1. As shown, remove the power supply plug from the AC adapter. 2. As shown, put on the power supply plug that you want to use. AC adapter BF-Type or A-Type 1. Remove the power supply plug. C-Type or S-Type 2. Put on an appropriate power supply plug.
Operation Turning the power off manually (The display is example.) Operation key Press and hold the Operation key on the pipette for at least five seconds until OFF is displayed on the display. Buzzer sounds (Three times), and the pipette turns the power off (OFF).
7-5 Parts names The following shows the each name of electronic pipette When confirming LCD, refer to “8-1 Display and functions” for details.
8. NAME AND FUNCTIONS OF DISPLAY AND KEYS 8-1 Display and functions Operation mode Aspirating and dispensing speed display Dispensing amount display Reverse operation mark Battery mark Buzzer mark Display of number Notice mark and a number of times Symbols Operation mode display Descriptions System setting Used to set up functions before mode pipetting, such as aspirating/dispensing speed, reverse operation, etc. (Refer to “9-4 System setting mode (SYS)”.
Symbols Aspirating and dispensing speed display Notice mark Buzzer mark Battery mark Descriptions Shows the speed level when aspirating or dispensing the liquid. ▲ blinks when aspirating, ▼ blinks when dispensing (Refer to “ 9-4 System setting mode (SYS)”.) When illuminated: Shows that volume calibration has been carried out. When flashing: Shows that weight mode for dispensing (mg) has been selected. (Refer to “11-3 Dispensing in a unit of weight (in mg unit)”.) Shows the buzzer is to sound or not.
9. FUNCTION AND HOW TO USE The MPA series have three modes, the standard mode (AUTO), multiple dispensing mode (MD) and the mixing mode (MIX). 9-1 Standard mode (AUTO) 1) Operating the standard mode This is a basic operation for pipetting. Aspirating one time and dispensing one time. This operation is the same as for a manual pipette. 2) Selecting the standard mode (The display is example.) [ 1 ] Press the [ 2 ] Press the [ 3 ] Press the (AUTO). Back key. Up or Down key to select ”AUTO”.
3) Operating the standard mode [ 1 ] Put the tip end into the liquid to be dispensed and press the Operation key on the pipette to aspirate the liquid. [ 2 ] Put the tip end into the receiving vessel and press the Operation key on the pipette to dispense the liquid. [ 3 ] Press the Operation key on the pipette to dispense the liquid remaining in the tip. (When the blowout function is ON ( ).
9-2 Multiple dispensing mode (MD) 1) Operation of the multiple dispensing mode This is a suitable function to dispense the same liquid continuously, such as when dispensing a liquid on a microplate, etc. The operation consists of aspirating one time and dispensing several times. When carrying out pre-rinse for multiple dispensing or stopping multiple dispensing, use the total discharge function. (Refer to “9-8 Total discharge function”) 2) Selecting the multiple dispensing mode (The display is an example.
[ 7 ] Press the count. Enter key to confirm the dispensing If you would like to change dispensing amount or dispensing count, press the Enter key before starting aspiration. When setting, operate from step 4 described above. 3) Operating the multiple dispensing mode The following example is when dispensing 20μL x 10 times. [ 1 ] Put the tip end into the liquid to be dispensed and press the Operation key on the pipette to aspirate the liquid. (The example shows 20μL x 10 times = approx.
[ 4 ] Repeat the operation described above with the dispensing count set. When the set count of dispensing operations is completed a buzzer will sound twice. (In the example, the buzzer sounds twice after 10 dispensing operations have been completed) [ 5 ] Press the Operation key on the pipette to discharge the remaining liquid. (In the multiple dispensing mode, reverse operation occurs automatically.
[ 3 ] Press the (MIX). Enter key to select mixing mode [ 4 ] Press the Up or Down key to set mixing count (One set with aspirating and dispensing). * Ten times maximum [ 5 ] Press the Enter key to confirm mixing count. [ 6 ] Press the Up or Down key to set mixing volume (Volume for aspirating when mixing). [ 7 ] Press the Enter key to confirm mixing volume. If you would like to change the mixing count or amount, press the Enter key before aspirating. When setting, operate from step 4 described above.
[ 3 ] The pipette executes the set count of aspirating-dispensing cycles by approx. 2/3 of the set mixing volume. Dispensing to absorption Repeat with mixing count set. [ 4 ] Press the Operation key on the pipette to dispense the liquid remaining in the tip. (When the blowout function is ON ( ).) [ 5 ] One mixing operation is completed once the aspirated liquid is completely discharged. When the operation is completed a buzzer sounds twice.
[ 2 ] Press the Up or Down key to select “SYS”. [ 3 ] Press the Enter key to enter the system setting mode. [ 4 ] By pressing the Up or Down key, change the setting of each item, and press the Enter key to confirm it.
9-5 Program setting mode The nine programs can be saved in the program memory built into the pipette (PROG 01 to 09). By saving a frequently used mode or volume for operation beforehand, these setting can easily be read out from the next use. Select and set the mode or volume to be saved before saving the program setting. Saving the program setting Set the pipette to your preferred settings. Example: When saving the AUTO mode, dispensing volume 200μL, buzzer ON and blowout ON (The display is example.
1 ) Setting the reverse mode (The display is example.) [ 1 ] Press the [ 2 ] Press the Back key. Up or Down Key to select "SYS". [ 3 ] Press the Enter key to enter the system setting mode. [ 4 ] Press the Enter key several times until is displayed. [ 5 ] Turn the blowout operation mode OFF using the Up/Down key, then confirm this selection by pressing the Enter key. [ 6 ] Turn the reverse operation mode ON using the Up/Down key, then confirm this selection by pressing the Enter key.
2) Operating the reverse mode [ 1 ] Press the Operation key on the pipette to aspirate the liquid. Aspirate [ 2 ] Press the Operation key on the pipette to dispense the liquid. Dispense [ 3 ] Press the Operation key on the pipette to discharge the remaining liquid.
9-8 Total discharge function Pressing and holding the Operation key expels all of the liquid remaining in the tip. This function is useful when, for example, you want to terminate the operation halfway through multiple dispensing. Continuing to hold the Operation key down after this turns the pipette power off. 9-9 “Dispensing correction function” for multiple dispensing The electronic pipette aspirates and dispenses a liquid by moving the internal piston up and down using motor.
11. CALIBRATING THE PIPETTE USING AN ELECTRONIC BALANCE The MPA series provide user with a dispensing volume calibration function. Using this function, it is easy to correct (calibrate) errors due to differences in tips used, etc. When you need to always control the dispensed volumes in a precise manner, perform volume calibration as necessary when you change the dispensing volume setting.
[ 3 ] Press the mode. Enter key to enter μL calibration [ 4 ] Press the Up or Down key to alter the value to the actual dispensed volume. [ 5 ] Press the Enter key to confirm actual dispensed volume. [ 6 ] The notice mark lights up to indicate that the volume calibration has been performed. (In this example, the dispensed amount is corrected and altered to 100μL.) * After calibrating, a volume range that can be selected may be limited depending on available movement range of the piston.
Volume calibration data resetting mode [ 3 ] Press the Up or Down key to select volume calibration data resetting mode. [ 4 ] Press the Enter key to enter volume calibration data resetting mode. [ 5 ] Press the ( Up or Down key to select means YES and . means NO.) [ 6 ] Press the Enter key to reset volume calibration data. [ 7 ] The Notice mark is turned off to indicate that volume calibration data have reverted back to the factory default.
Method for selecting the mg unit [ 1 ] Press the Back key to enter the mode to select an operation mode. [ 2 ] Press the Up or Down key to select the system setting mode “SYS”. [ 3 ] Press and hold the Enter key for approx. three seconds. [ 4 ] The unit currently selected is displayed. [ 5 ] Press the Up or Down key to select the unit. [ 6 ] Press Enter key to confirm. (Unit in μL) (Unit in mg) mg With theμL unit selected, the Notice mark is turned off while the μL unit mark lights up.
Method for mg calibration 1. Select the mg unit beforehand. (Refer to “11-3 Dispensing in a unit of weight (in mg unit)”) 2. Set the dispensing amount of the MPA series to the weight to which you would like to calibrate it. (The example is 100mg) 3. Using an electronic balance, measure and record an actually dispensed weight as opposed to the selected dispensing amount setting. (The example is 95mg) 4. Enter an actual dispensed weight to the pipette by the following procedure.
12. ADJUSTING HEIGHT OF THE TIP EJECTOR A height of the tip ejector can be adjusted so that it can match the conditions of how the tip used was connected. Use a small minus screwdriver. By turning the adjustment screw in a counter-clockwise direction, the tip ejector can be lowered toward the tip side. Adjustment screw Expanded 13.
13-1 Autoclave By removing the lower part of pipette from the pipette, it can be placed in an autoclave. Condition for autoclave: Lower part of pipette 121℃ 2 atm 20 minutes Removing the lower part of pipette - The piston and piston rod are connected using a magnet. Therefore, by removing the connection nut completely, the lower part of pipette can be removed by pulling downward. Connection nut Piston rod Magnet Lower part of pipette - The pipette has a strong magnet.
14. TROUBLE SHOOTING The electronic pipette has a self-check function. When there is an error in these results, the pipette displays a following error massage “Err XX”. When an error cannot be released even after applying a following remedy to the pipette, please apply for a repair. (Refer to “15. WHEN REQUESING REPAIRE”.) Circumstances “Err 01” Considerations for cause The automatic position resetting is not working. “Err 02” Step motor error. The sample liquid lingers inside the pipette tip.
15. WHEN REQUESTNG REPAIRE The pipette requires repair if an error occurs and cannot be corrected by following the troubleshooting methods provided in this manual. In this case, please contact your local A&D representative. When requesting repairs, it is essential that you confirm the pipette is free of contamination by a harmful material.
16. SPECIFICATION MPA-10 MPA-20 MPA-200 MPA-1200 0.5 to 2.0 to 10 to 100 to Volume range 10.0μL 20.0μL 200μL 1200μL 1.0μL 10.0μL 2.0μL 20.0μL 10μL 200μL 100μL 1200μL Performance Volume ±4.0% ±1.0% ±4.0% ±1.0% ±2.5% ±0.6% ±2.5% ±0.5% Accuracy *1 *2 0.4% 2.5% 0.4% 1.0% 0.15% 0.6% 0.15% Repeatability (CV) 2.
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17-2 Tips and accessories Number Applicable pipette Name MPA-10 MPA-20 MPA-200 MPA-1200 Tip box with locking parts *4 *5 AX-BOX-200A ○ ○ (10/20/200μL) Tip box with locking parts *4 *5 AX-BOX-1200A (1200μL) Tip box *4 *6 AX-BOX-200B ○ ○ (10/20/200μL) ×10 set Tip box *4 *6 AX-BOX-1200B (1200μL) ×10 set Tip cartridge *7 *8 (10/20μL)×10 set AX-CART-10/20 ○ ○ A&D 10/20μL Standard tip Tip cartridge *7 *8 (200μL)×10 set AX-CART-200 A&D 200μL Standard tip Tip cartridge *7 *8 (1200μL)×10 set AX-CART-1200 A&D 12
○AX-BOX-1200B Tip box Upper case Lower case ○AX-CART-1200 Tip cartridge (1200μL) Consultation) The tip is marked with lines to act as guidelines for the aspiration amount.
17-3 Disposable items (User replaceable) Number AX-LOW-10 AX-LOW-20 AX-LOW-200 AX-LOW-1200 AX-BAT-MPA Applicable pipette Name MPA-10 Lower part MPA-20 MPA-200 MPA-1200 ○ (10μL) Lower part ○ (20μL) Lower part ○ (200μL) Lower part ○ (1200μL) Battery ○ ○ ○ ○ ○ ○ ○ ○ AC adapter AX-TB265 (Provided as standard) Battery Lower part AC adapter 42
17-4 Others - Leak tester AD-1690 Leakage within the pipette can be easily checked.
MEMO 44
Attestation of contamination removal Please fill in the following items when you send a pipette for repair. Model name: Serial number S/N: I attest to the fact that this pipette is free of contamination by any substances that could pose a health threat to humans, such as Infectious bacteria or viruses, radioactive substances with associated risks of exposure, toxic substances, etc.
3-23-14 Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013, JAPAN Telephone: [81] (3) 5391-6132 Fax: [81] (3) 5391-6148 A&D ENGINEERING, INC. 1756 Automation Parkway, San Jose, California 95131, U.S.A.
