Leica CV5030 Robotic Coverslipper Instructions for Use Leica CV5030 Order No. 14 0478 80101 RevE V 3.0, English - 04/2013 Always keep this manual with the instrument. Read carefully before working with the instrument.
NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Contents 1. 2. 3. 4. 5. 4 Important Information.....................................................................................................7 1.1 1.2 1.3 1.4 Symbols and their meanings....................................................................................................... 7 User group...................................................................................................................................... 9 Intended use of instrument.................................
Contents 5.9 5.10 5.11 5.11.1 5.12 5.13 5.13.1 6. 7. Setting parameter sets............................................................................................................... 57 Menu A – parameter settings.................................................................................................... 57 Menu B – parameter settings.................................................................................................... 60 Leaving the parameter and submenu........................
Contents 7.7.3 Transfer arm of the TS5015 or TS5025 Transfer Station for workstation operation......... 87 7.8 Procedure for changing the cover slip mountant.................................................................. 88 7.8.1 Changing from one xylene-based coverslip mountant to another...................................... 88 7.8.2 Changing from a xylene substitute coverslip mountant to a xylene-based mountant..... 88 7.8.3 Changing from a xylene-based coverslip mountant to a xylene substitute...
1. Important Information 1.1 Symbols and their meanings Warnings appear in a gray box and are marked by a warning triangle . Notes i.e. important user information appear in a gray box and are marked by an information symbol . Inflammable solvent and reagents are marked with this symbol. Instrument surfaces which become hot during operation are marked with this symbol. Avoid direct contact to prevent risk of burning. (5) Numbers and parentheses refer to item numbers in the illustrations.
1. Important Information 1.1 Symbols and their meanings REF Order No. SN Serial number Observe the Instructions for Use The package contents are fragile and must be handled with care. Indicates the correct upright position of the package. The package must be kept in a dry environment. It is not permitted to stack packages, and no loads may be placed on top of the package. Indicates the temperature range to which the medical device can be safely exposed.
1. Important Information 1.2 User group • The Leica CV5030 may be operated by trained laboratory personnel only. • All laboratory personnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it. 1.3 1.4 Instrument type All information provided in these Instructions for Use applies only to the instrument type indicated on the cover page.
2. Safety 2.1 Safety notes • The safety and caution notes in this chapter must be observed at all times. • Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. • The protective devices on the instrument and its accessories must not be removed or modified. Only qualified service personnel authorized by Leica may repair the instrument and access its internal components.
2. Safety 2.2 Warnings (continued) Safety instructions - transport and installation • • • • • • • • • The instrument must be transported in an upright position only (use transport anchors!). Two persons are required when lifting or carrying the instrument! The Leica CV5030 is intended for use in enclosed rooms only. The instrument may be operated with the provided power cable only. This power cable may not be replaced by another.
2. Safety Safety instructions - working with the instrument (continued) • Operate the instrument with the exhaust hose and connection on an external laboratory extraction or under a suitable fume hood. In the process, the corresponding active carbon filter should be used for auxiliary support. • Since the instrument is intended to be operated with a solvent, there is a fire hazard if work with an exposed flame (e.g. Bunsen burner) is carried out in the direct vicinity of the instrument.
3. Instrument Components and Specifications 3.1 Overview - instrument components Unit cover Output station for output magazines Pick & Place module (cover slip mount) Output magazines Maintenance door Bottle for coverslip mountant Cover slip magazine Dispenser group Dispenser rest (park) position (prime position) Control panel Main switch Loading bath Height-adjustable instrument feet Input door Loading drawer Fig.
3. Instrument Components and Specifications 3.2 Technical data Nominal supply voltages: Nominal frequency: Approvals Fuse: Nominal power: Dimensions (W x H x D): 100 - 240 V AC, ± 10 % 50 - 60 Hz CE, CSA C/US Automatic fuseT5A 100 VA Hood closed: 420 x 600 x 550 mm Hood opened: 420 x 980 x 550 mm Width (from the left to the right base): 370 mm Depth (from the posterior base to the front base): 525 mm Multistainer workstation: Hood opened: 1620 x 980 x 760 mm Weight (without packaging material): approx.
3. Instrument Components and Specifications 3.2 Technical data (continued) Performance: Specimen slide throughput: Usable specimen slides: Cover slip magazine capacity: Cover slips: Mountant bottle capacity: Max. fill volume: Coverslip mountant application quantity: Mountant types: Specimen slide holders: Output magazines: 3.3 1 specimen slide in approx. 9 sec. All commercially available specimen slides per ISO standard 8037-1. Leica recommends the use of validated Surgipath™ specimen slides.
3. Instrument Components and Specifications 3.3 Standard delivery—packing list (continued) 1 Accessory kit, consisting of: 14 0478 39734 1 Leica brush 14 0183 30751 1 Allen key, No. 3.0 14 0222 04138 1 Screwdriver 5.
4. Instrument Setup 4.1 Installation site requirements The location for the Leica CV5030 Robotic Coverslipper must meet the following requirements: • The installation location must be well ventilated, and must contain no sources of ignition of any kind. • The chemicals used in the Leica CV5030 are easily inflammable and pose a health hazard. • Never operate the instrument in rooms with an explosion hazard.
4. Instrument Setup 4.1 Installation site requirements (continued) • The instrument operator has to ensure that ESD safety precautions are maintained. • The instrument operator is obligated to conform to the local workplace limit values and to document them. Furthermore, the instrument operator must ensure that there is sufficient air exchange and the active carbon filter is changed at the recommended interval.
4. Instrument Setup Unpacking the CV5030 (continued) 4.2 Removing the accessories (Fig. B) 7 • Remove the two screws (Fig. A, 4) in the side panel (left and right) and take out the transport anchor (5). • The box with accessories (6) can now be removed from the shipping package. 6 5 Fig. B Removing the instrument • Unscrew the 8 screws (Fig. A, 3) on the bottom of the wooden box on the exterior. Carefully remove the wooden box from the baseplate.
4. Instrument Setup 4.2.1 Setting up the CV5030 21 Fig. 3 4.3 65 20 • Grab hold of bottom of the instrument from the front and rear (with at least 2 people; the instrument weighs approx. 57 kg) and place it on a stable laboratory table. • When doing so, ensure that the instrument is standing on all four feet. • Pull the plastic protective hood off of the instrument by pulling upwards and remove the two adhesive strips (20). • Remove the foam safeguard (21) from the output station.
4. Instrument Setup 4.3.1 Removing or installing the transport anchors • Open the service door (28) on the left side of the instrument and remove the foam (50). 50 28 Fig. 4 1. Remove the transport anchor (24) for the Pick & Place module. 2. Remove the transport anchor (25) for the gripper. The gripper slowly moves downward in the process. • Using the provided No. 3 Allen key, unscrew the screws (22) and (23) for the two red transport anchors (24) and (25) (see Fig.
4. Instrument Setup 4.4 Leveling the instrument • Move the instrument to its final location on the work surface. When doing so, ensure that all four feet are on the work surface. 26 • Open the hood and place a suitable spirit level (26) onto the work surface as shown in Fig. 6. 27 Detail 2 • The instrument can be leveled in both directions by screwing or unscrewing the instrument feet (27) (detail 2 in Fig. 6). Fig. 6 4.
4. Instrument Setup 4.5 Exhaust system (continued) 70 Inserting the active carbon filter Detail: • Unscrew the slotted screw (34) at the service door (28) on the left side of the instrument and open the service door to the left (Fig. 7). • Unscrew the screw (29) of the filter cover (30) using a No. 3 Allen key and pivot the cover upwards. • The insertion date can be written down on the adhesive label (31). • Insert the filter (32), close the cover (30) and secure it back in place using the screw (29).
4. Instrument Setup 4.6 Installing the dispenser group • Take the dispenser group (Fig. 8) out of the packaging. • Insert the dispenser (35) into the holder (39) for the prime position (refer to Fig. 9). • Insert the pressure hose (36) from the cover of the mountant bottle (40) into the compressed air output (41) up to the stop inside. • To take the pressure hose back out, press the white ring (42) down and pull out the pressure hose.
4. Instrument Setup 4.6 Installing the dispenser group (continued) 45 49 48 46 44 Fig. 10 • Finally, insert the cable and the air hose into the holder (45) provided (Fig. 10) Dispenser needles 47 21 G 20 G 18 G 16 G Fig. 11 Inserting the dispenser needle (Fig. 11) • Select the dispenser needle to be used for coverslipping from the scope of delivery (Fig. 12).
4. Instrument Setup 4.7 Aligning the dispenser needle height relative to the specimen slide outfeed 4.7.1 Checking needle height Detail: 55 52 28 51 53 Fig. 13 The height of the dispenser needle must be aligned to the specimen slide correctly so that no air bubbles are generated while the mountant is applied. The dispenser needle height must not be set too low in order to avoid damage to the specimen on the specimen slide. Aligning the dispenser needle: • Switch off the instrument and unplug it.
4. Instrument Setup 4.7.2. Setting the needle height The needle height has to be corrected after reinserting the dispenser needle. 35 39 Fig. 15 56 57 Setting the needle height: • Reset the dispenser (35) from the working position (see Fig. 16, 57) to the rest position (see Fig. 15, 39). • There is a screw (see Fig. 16, 54) in the working position. This determines the distance between the dispenser needle and specimen slide.
4 Instrument Setup 4.7.2 Setting the needle height (continued) • The dispenser is reset into the rest position if the 0 mm distance is set correctly. • Now carry out a three-quarters counterclockwise revolution using the No. 3 Allen key. • This achieves the optimum distance of 0.75 0.8 mm. • The set height of the dispenser needle can be inspected again by a visual inspection from the front (see Fig. 14). • Then close the service door, screw it down tight and reconnect the instrument to the power supply.
4. Instrument Setup 4.8 Dispenser needle cleaner (nozzle cleaner) The dispenser needle cleaner is used to clean excess coverslip mountant off of the dispenser needle after each specimen slide processed. 1 3 7 5 9 8 4 10 6 2 Fig. 17 Parts Remove the individual components from the packaging and check them for completeness.
4. Instrument Setup 4.8 Dispenser needle cleaner (nozzle cleaner) (continued) Assembly 12 To install the holder for the dispenser needle cleaner, you must first remove the transport anchors for the Pick & Place module (see Chap. 4.3.1, Fig. 5). 9 11 4 ① ➁ For installation, use the threaded holes (11) of the transport anchor (see Fig. 19/1). Fig. 19 7, 8 • First, fasten the mounting bracket (4) in the bores (11) in the housing wall (12) using the two screws (9) (Fig. 19/2).
4. Instrument Setup 4.8 Dispenser needle cleaner (nozzle cleaner) (continued) Alignment to the dispenser needle • After the dispenser needle cleaner is installed, it must be aligned relative to the dispenser needle (13). • To do so, unscrew the screw (7) using the No. 3 Allen key (6) (Fig. 20/1) and align the dispenser needle cleaner by moving the bracket (3) sideways until the dispenser needles (13) move into the correct position in the middle of the cleaning port (Fig. 21). 13 7 3 Fig.
4. Instrument Setup 4.9 Connecting the power supply The electrical connection is on the left rear side of the instrument (Fig. 23). The unit is dimensioned for: 100 to 240 V AC voltage at 50/60 Hz. The instrument MUST be connected to a grounded power socket. Only use a suitable cable that is appropriate for the local power supply (plug must fit on-site wall outlet).
4. Instrument Setup 4.10 Installing the accessories (continued) 62 61 Fig. 25 63 64 Fig. 26 67 68 65 66 Coverslip magazine • Select and fill a cover slip magazine (62) for the desired cover slip type (22 or 24 mm width). • Tap the cover slip magazine on the edge that points toward the front of the instrument after insertion (this ensures that all of the cover slips are positioned correctly).
4. Instrument Setup 4.11 Refilling consumables 40 44 62 69 Fig. 28 • • • 34 Fill the loading bath (Fig. 27, 68) with a solvent compatible with the coverslip mountant. The level should reach up to the specimen slide's label field. Fill the glass vial (69) in the rest position with a solvent compatible with the coverslip mountant (approx. 10 ml). The dispenser needle must always be immersed in the solvent.
4. Instrument Setup 4.11 Refilling consumables (continued) • • • After filling the mountant bottle, check the bottle neck and threading for cover slip mountant residue and clean it if necessary. To achieve optimal coverslipping quality, leave the filled mountant bottle under a fume hood for 6 - 12 hours covered by a loosely positioned, blue bottle lid. Only insert the bottle into the instrument after doing this. Screw the red lid for the dispenser group tightly onto the mountant bottle.
5. Operation 5.1 Control panel functions The control panel Fig. 29 is provided as an example only and shows a valid start display for this instrument. Fig. 29 The control panel field consists of a membrane keyboard with ten pushbuttons, four LED indicators and a two-line LCD display. • It is used to control the functions of the instrument and to display the software status. • The control panel field indicates the current status of the robotic coverslipper and operations in progress.
5. Operation 5.2 Key functions for instrument operation START begins the coverslipping operation. This is only possible from READY or PAUSE mode. LED The "READY" LED is illuminated in green in READY mode and red in PAUSE mode. Pressing PAUSE interrupts the coverslipping operation (BUSY LED is illuminated). Processing of the current specimen slide finishes before the instrument switches to PAUSE mode. LED The "READY" LED is illuminated in red afterwards. PAUSED appears in the display.
5. Operation 5.2 Key functions for instrument operation (continued) RELEASE COVER SLIP RELEASE COVER SLIP ventilates the Pick & Place module's suction cups. The cover slip is released. The function is disabled while the BUSY LED is illuminated in green. RELEASE SLIDE Pressing RELEASE SLIDE opens the gripper jaws as long as the button is held down. The function is disabled while the BUSY LED is illuminated in green. PRIME Pressing the PRIME key opens the dispensing valve as long as the key is pressed.
5. Operation 5.3 Switching the instrument on or off ð Move the power switch on the front side of the instrument to the ON = I or OFF = 0 position. This is followed by a beep. The instrument initializes after being switched on. The installed software version is shown in the display. The "READY" LED is illuminated in red. LED "CHECK BATH" is the prompt for the user to insert the loading bath after initialization. To do so, open the loading door and pull out the loading drawer.
5. Operation 5.3 Switching the instrument on or off (continued) A loaded specimen slide holder can now be inserted into the loading bath. After inserting it, push the loading drawer back and close the loading door. The message "PRIME POSITION" appears. Take the dispenser out of the rest position; the message "PRIME TO ACT." is displayed. Important! In general, when inserting or removing the dispenser, make sure that caution is used during movements at the time of insertion or removal.
5. Operation 5.4 Brief inspection before starting the coverslipping operation • Push the loading drawer (68) with the loading bath back into the instrument; close the loading door (65) afterwards. • Furthermore, check and, if necessary, correct the following before starting the coverslipping operation: - Filling of the dispenser needle cleaner. - Level of the coverslip mountant bottle. - Sufficient filling and correct positioning of the cover slip magazine. - Presence of the output magazines.
5. Operation 5.5 The coverslipping operation After complete initialization, the prime cycle and carrying out preparations (refer to Chapter 5.3), the coverslipping operation can be started by pressing the START button ("READY" LED is illuminated in green). • The loading bath is transported into the position required for the coverslipping operation and the output magazine moves into position.
5. Operation 5.5 The coverslipping operation (continued) • If some or all specimen slides are to be removed, use the LIFT key to raise the output magazines to the removal position. Removal is not absolutely necessary after each specimen slide holder, as long as sufficient capacity remains in the output magazine for the next coverslipping cycle (max. capacity = 60 specimen slides).
5. Operation 5.6 Interrupting the coverslipping operation Press PAUSE to interrupt the coverslipping operation. ò • The currently processed specimen slide is completely coverslipped and pushed into the output magazine, accompanied by the message "PAUSING". • If this step is ended, the message "PAUSED" is shown in the display. A signal tone is emitted at the same time. • Confirm the signal tone by pressing the RESPOND button. Whenever the instrument is to pause only briefly (e.g.
5. Operation 5.6 Interrupting the coverslipping operation (continued) Emergency stop using STOP. ò • In an emergency or if the coverslipping operation is to be canceled quickly, press the STOP button. Then, the coverslipping operation is interrupted immediately. • The message "STOPPED" is displayed accompanied by a signal tone. • Confirm the signal tone with RESPOND. Remove all glass parts or other objects away from the CV5030 working area after pressing STOP. Only then may START be actuated.
5. Operation 5.6 Interrupting the coverslipping operation (continued) Empty coverslip magazine If the number of cover slips falls below the limit, the message "COVERSLIPS LOW" appears on the display. The instrument continues to operate until there are no more cover slips in the cover slip magazine. In this case, the messages "PAUSED" and "CS EMPTY" are shown on the display and a signal tone is emitted. • Switch off the signal tone with RESPOND.
5. Operation 5.6 Interrupting the coverslipping operation (continued) Output magazine full The coverslipping operation is interrupted if all of the positions in the output magazine are filled by specimen slides. The instrument is in PAUSE mode. • The READY LED is illuminated in red and PAUSE is green. The display shows "PAUSED CHECK LIFT", accompanied by a signal tone that shows that the output magazine is missing or has to be emptied. • Confirm the signal tone with RESPOND.
5. Operation 5.6 Interrupting the coverslipping operation (continued) Pressure or vacuum cannot be reached If the dispenser system cannot reach the intended pressure or vacuum after a certain time, the instrument automatically switches to PAUSE mode. • The READY LED is illuminated in red, the PAUSE LED is green. Depending on the error that has occurred, the display reads "PAUSED BOTTLE VACUUM" or "PAUSED BOTTLE PRESSURE", accompanied by an signal tone indicating that coverslipping cannot continue.
5. Operation 5.6 Interrupting the coverslipping operation (continued) Cover slip missing If the Pick & Place module loses or breaks a cover slip on the way to the specimen slide that needs to be covered, the instrument automatically switches to PAUSE mode. In PAUSE mode, the Pick & Place module can be moved forward as necessary, toward the user, to make the specimen slide accessible. • The READY LED is illuminated in red, the PAUSE LED is green.
5. Operation 5.7 Display indicators and instructions The display of the Leica CV5030 is used to display status and error messages that provide information about the current status of the instrument. Status messages always appear in the bottom line of the display. The top line displays the operating status of the instrument. The following table compiles all status messages. In addition, it indicates to the user which action to carry out.
5. Operation 5.7 Display indicators and instructions (continued) Display Explanation Action The user is prompted to press the PRIME button again in order to pump mountant from the dispenser. Hold the PRIME button down until mountant comes out of the dispenser without bubbles. Check whether there are still air bubbles in the dispensing group's coverslip mountant hose. If air bubbles are still present, press PRIME until they have been removed.
5. Operation 5.7 Display indicators and instructions (continued) Display Explanation Action The instrument is in pause mode. Fill the cover slip magazine. The coverslipping process was Pressing START allows work to interrupted due to the empty continue. cover slip magazine. The instrument is in pause mode. Insert a loading bath into the There is no loading bath in the loading drawer. Press RESPOND loading drawer. as confirmation, then press START to continue coverslipping.
5. Operation 5.7 Display indicators and instructions (continued) Display Explanation Action At least three cover slips in a Check the cover slips in the row are defective. cover slip magazine. If the messages occurs repeatedly despite the cover slips being intact, clean the cover slip sensor. If the message persists, clean the Pick & Place module (skids and suction cups). The user has pressed the STOP The output magazine, if loaded, button. has to be removed from the instrument.
5. Operation 5.7 Display indicators and instructions (continued) Display Cause Action The Pick & Place module loses As soon as the display reads "CS the coverslip on the way to the DROPPED", the instrument goes automatically into PAUSE mode. coverslipping position. Notice: Move the dispenser into the park position, move the Pick & Place module towards the user and remove the specimen slide. You can now coverslip the specimen slide manually.
5. Operation 5.7 Display indicators and instructions (continued) The following status messages are displayed only if the instrument is connected to a staining machine (Leica ST5010 or Leica ST5020) via a transfer station. Display Explanation Action Indicates that a specimen slide Wait for the specimen slide to holder that has been transferred finish processing. from the staining machine is in the process of coverslipping.
5. Operation 5.8 Button functions for programming Press and hold RESPOND for approx. two seconds to set the programs and parameter sets the instrument uses to operate. Press and hold for 2 s 72 71 Fig. 32 Press 1 x The individual parameter sets are set using two submenus: Menu A (Fig. 32) and Menu B (Fig. 33) Toggle between the two submenus by briefly pressing RESPOND. The active submenu (71) is displayed in the right half of the top display line.
5. Operation 5.9 Setting parameter sets There are a total of four programs (SET 1 to SET 4) that can be stored in the instrument with different parameter settings. Pressing the START button calls up the next higher program. Pressing the Pause button calls up the next lower program (Fig. 34). ñ ò Fig. 34 5.10 Menu A – parameter settings The individual buttons used to select and program the respective parameters are in the bottom row of the control panel field.
5. Operation 5.10 Menu A – parameter settings (continued) Mountant Stroke – stroke length of the coverslip mountant The quantity and stroke length of the coverslip mountant is adapted to the cover slip length. Label field Mountant Stroke: 40 = 40 mm Cover slip length Mountant Stroke: 50 = 50 mm stroke length Cover slip length Mountant Stroke: 55 = 55 mm Cover slip length Mountant Stroke: 60 = 60 mm Cover slip length Fig.
5. Operation 5.10 Menu A – parameter settings (continued) Mountant type - dispensing pressure The pressure needed to dispense the coverslip mountant is set in increments of 1: ð 1 = 100 mbar/lowest pressure to 10 = 1000 mbar/highest pressure An increase of "1" increases the pressure by 100 mbar. The pressure has to be adapted based on the coverslip mountant used (different viscosities) and the dispenser needle used (different diameters).
5. Operation 5.11 Menu B – parameter settings Coverslip Position Corr - correction of the cover slip placement position The cover slip placement position can be modified using the CSP (91) (cover slip placement position) parameter (see Fig. 38). If the cover slip placement position is to be moved towards the edge of the specimen slide, then select a negative value between –5 and –30.
5. Operation 5.11 Menu B – parameter settings (continued) Speaker Volume Level - volume of the audible signal ð Setting for the volume of key tones, messages and the signal tone: In increments of 1 from 1 (quiet) to 3 (loud) The signal tone and audible signals of the buttons cannot be disabled completely.
5. Operation 5.11 Menu B – parameter settings (continued) Coverslip Limit Adjust - setting for the cover slip limit (continued) The Pick & Place module moves to the cover slip magazine and lowers itself down to the top cover slip. Then it returns to its original position. The setting for the new cover slip limit has now been saved and the overview for the parameter setting in Menu B reappears on the display.
5. Operation 5.12 Recommendation for parameter setting (beginning with firmware 3.01.04) The following settings serve as recommendations and have to be adapted to the conditions in the respective lab. The parameter settings have been determined as follows under standardized condition in a Leica Biosystems Nussloch GmbH lab: • Use of tissue sections with a thickness of 3-5 µm. • Room temperature: 20-25 °C. • Use of cover slip with a size of 24 x 50 mm and thickness #1.
5. Operation 5.12 Recommendation for parameter setting (beginning with firmware 3.01.
5. Operation 5.12 Recommendation for parameter setting (beginning with firmware 3.01.04) (continued) Designation Manufacturer/ Loading bath supplier filled with VOL TYPE LEN DPX Fluka Xylene 18 50 6 6/7 0 Pertex Medite Xylene 18 50 7/8 8 0 Mounting Medium No. 4111/4112 Richard-Allan Scientific Xylene 21 50 1/2 5/6 -30/ -40 Cytoseal Xyl Richard-Allan Scientific Xylene 21 50 2/3 5 0 Cytoseal 60 (280) Richard-Allan Scientific Xylene 20 50 4/5 6/7 -20 Eukitt O.
5. Operation 5.13 Determining the optimum parameter setting (menu A+B) The following procedure provides assistance for: • Adjusting the previously listed parameter setting to onsite lab conditions. • Determining the optimum parameter settings for an unlisted coverslip mountant (refer to Chapter 5.12). • Changing from coverslipping with solvent ("wet") to coverslipping without solvent ("dry") in the loading bath, or, reversed (refer to Chapter 5.13.1 - 3. Differences between "wet" and "dry" coverslipping).
5. Operation 5.13.1 Procedure (continued) • In contrast to "wet" coverslipping, higher application quantities of mountant are necessary for "dry" coverslipping, i.e. the parameters VOL and/or TYP must be increased as needed. • If this is no longer possible, a dispenser needle with larger diameter can also be used. 4 Preparing the coverslipping machine • Be mindful of air-tightness when inserting the coverslip mountant bottle (refer to Chapter 4.11).
5. Operation 5.13.1 Procedure (continued): If artifacts become visible within the monitoring process (streaks, cloudiness, discoloration), the involved reagents must be inspected for compatibility and storage life. The staining protocol should be adapted as needed. 7. Inputting the first test parameters: 7 a) Effect of parameters on the application quantity • STK - Stroke length of the coverslip mountant corresponding to the coverslip length used. • VOL - Quantity of the coverslip mountant: 1 (min.
5. Operation 5.13.1 Procedure (continued): Important note: If TYP is at the maximum setting (10 - highest pressure), it is possible to generate small air bubbles in the dispenser system. This depends on specific properties of the respective coverslip mountant and the selection of dispenser needle (frequently occurring with small-diameter needles). Therefore it is advisable to begin with the mid-range parameter settings when determining the optimum parameter sets.
5. Operation 5.13.1 Procedure (continued): 8.
5. Operation 5.13.1 Procedure (continued): Notes on fine-tuning the parameters STP, CSP, LEN: • In most cases, the parameter STP must be set in the positive range at approx. +20 to +40 for extremely low-viscosity coverslip mountants (information is based on experimental values and may vary). For lower values (negative value up to 0/+10), there is the danger that the coverslip mountant will flow out over the specimen slide or coverslip edge.
6. Workstation operation You have the ability to connect the robotic coverslipper to a Leica ST5010 (Autostainer XL) or ST5020 (Multistainer) staining machine to create a workstation. 6.1 Operation as ST5010 – CV5030 workstation Both instruments are connected to each other via a TS5015 Transfer Station. • Specimen slide holders (77) are conveyed from the unload station (75) into the transfer station by a transfer arm (76). • The message "STAINER PROC." appears on the display.
6. Workstation operation 6.1 Operation as ST5010 – CV5030 Workstation (continued): • All empty specimen slide holders must be removed from the discharge chute and the drawer of the transfer station must be closed. Then, press the START button so that the "READY" message appears on the display. Transfer from the slide stainer to the robotic coverslipper is now possible once again.
6. Workstation operation 6.2 Operation as ST5020 – CV5030 workstation In this case, both instruments are connected to each other by a TS5025 Transfer Station. Unlike the ST5010 workstation, both instruments - the ST5020 and CV5030 - communicate with each other. Rear side of the ST5020 Rear side of the CV5030 • For the communication between the stainer and coverslipper, insert the 59 serial interface cable (87) included in the standard scope of delivery into 86.
6. Workstation operation 6.2 Operation as ST5020 – CV5030 workstation (continued): Detail: Transfer of the specimen slide holder to the transfer station 81 82 79 80 77 • After the coverslipping operation, the empty specimen slide holder is transferred back onto the discharge chute (81) of the transfer station. The display shows "READY". • If the discharge chute is already occupied with two empty specimen slide holders, an additional "TS FULL" message is shown on the display of the CV5030.
6. Workstation operation 6.3 Important instructions for operation as workstation 81 83 Fig. 44 • Specimen slide holders loaded manually into the CV5030 must always be removed immediately after processing. • The unloading chute (Fig. 44, 81) of the transfer stations should be emptied after the "TS FULL" message appears. At the latest, this should be done after a signal tone that occurs after the third specimen slide holder and the "PAUSED" and "TS FULL" messages.
6. Workstation operation 6.3 Important instructions for operation as workstation (continued): Important instructions for operation of different specimen slide holders The default specimen slide holders intended for workstation operation are Leica plastic holders for 30 specimen slides (Order No. 14 0475 33643). Use of other Leica specimen slide holders (e.g. Leica plastic holders for 20 specimen slides) or specimen slide holders from other manufacturers is not permissible in workstation operation.
6. Workstation operation 6.4 Interrupting workstation operation • The PAUSE and STOP buttons have mostly the same functions outlined in Chapter 5.6. • If the coverslipping operation is interrupted using the STOP button, the coverslipper must be initialized. To do so, press the START button. • After the coverslipping operation is interrupted using the STOP button, the specimen slide holder, which is still in the instrument, must be removed from the loading bath manually after processing is finished.
7. Cleaning and Maintenance 7.1 Notes on cleaning and maintenance • The instrument should always be cleaned after the end of work, but BEFORE the instrument is switched off. • The regular maintenance intervals must be observed. • The deep loading bath included in the scope of delivery must be taken out of the instrument BEFORE the end of work and not reinserted until after switching on and initialization. This prevents changes to the settings and damage of the gripper.
7. Cleaning and Maintenance 7.1 Notes on cleaning and maintenance (continued) Notes on cleaning agents • Clean the hoods and the housing with mild and pH-neutral commercial household cleaners. The finished surfaces have only limited resistance to solvents! • Clean the cover slip sensors, suction cups, skids of the Pick & Place module, transport chain, specimen slide holder and output magazines using a lint-free cloth soaked in compatible solvent.
7. Cleaning and Maintenance 7.2 Daily cleaning and maintenance - overview (continued) G Check the Pick & Place module, skids (refer to Chapter 7.5.8), suction cups (refer to Chapter 7.5.9) and cover slip sensor (refer to Chapter 7.5.10) for: • Coverslipping mountant residue • Broken glass If necessary, replace the suction cups with new ones and clean the skids using a compatible solvent.
7. Cleaning and Maintenance 7.4 Cleaning and maintenance as necessary A Filling the coverslipping mountant bottle: • If possible, top up the coverslip mountant at the end of the working day so that any air bubbles that arise during filling are degassed by the next working day. • The coverslip mountant needs between 6-12 hours of resting time to degas (time depends on the type of the coverslip mountant).
7. Cleaning and Maintenance 7.5 Description of the required daily cleaning measures (continued) 7.5.3 Glass vial in the dispenser rest position • Check the level, empty if necessary and add a compatible solvent. 7.5.4 Loading bath • Fill the loading bath with a sufficient amount of solvent. 7.5.5 Dispenser needles • Before starting up the instrument and before the priming cycle, check the dispenser needles for proper flow and adhesive residue.
7. Cleaning and Maintenance 7.5 Description of the required daily cleaning measures (continued) 7.5.9 Cleaning and replacing the suction cups 7.5.10 Cover slip sensor 89 88 Fig. 47 Fig. 46 • Check the suction cups (Fig. 46, 88) for small glass shards and splinters (from glass breaking) and adhesive residue. Carefully remove any clinging adhesive residue and glass shards and splinters. Carefully wipe down the suction cups using a lint-free cloth soaked in compatible solvent.
7. Cleaning and Maintenance 7.6 Description of the required weekly cleaning measures 7.6.1 Dispenser group We recommend rinsing the dispenser group once a week using approx. 150 ml of a solvent that is compatible with the coverslip mountant. Notice: Due to the high pressure when rinsing with solvent, it is necessary to wear gloves, safety goggles and appropriate protective clothing when changing the cover slip mountant! Select an adequately sized container to catch the rinsing residue.
7. Cleaning and Maintenance 7.6 Description of the required weekly cleaning measures (continued) 7.6.2 Dispenser needle cleaner (nozzle cleaner) 7.6.3 Specimen slide holder, gripper and output magazines • Carefully clean the gripper using a lint-free cloth soaked in compatible solvent (if necessary, also daily if using labels that protrude or are sensitive to solvent). • Check the output magazines for clinging and dried coverslipping mountant.
7 Cleaning and Maintenance 7.7 Description for cleaning and maintenance as necessary 7.7.1 Active carbon filter • Replace the active carbon filter with a new one according to the description in Chapter 4.5. The active carbon filters provided for the replacement should be stored only unopened and in their undamaged protective plastic bag. 7.7.
7. Cleaning and Maintenance 7.8 Procedure for changing the cover slip mountant Before switching the mountant, switch off the instrument and disconnect it from the power supply! Remove the loading bath and specimen slide holders from the instrument before switching it off.
8. Malfunctions and Troubleshooting 8.1 Error codes All of the error messages with the corresponding actions for troubleshooting are listed below. If faults occur that cannot be corrected with the procedures recommended in the table, or if they occur again, contact the responsible Leica Service contact person. In case of errors, generally the user's first step is to secure the specimens located at different positions in the instrument/workstation while ensuring personal safety.
8. Malfunctions and Troubleshooting 8.1 Error codes (continued) Display Cause Troubleshooting Error 313 TS-Z BLOCKED The transfer arm of the TS5025/5015 Transfer Station is blocked in its up/ down movement (Z-axis). Remove the cause of the blockage (specimen slide holder) and inspect the transfer arm of the TS5025/TS5015. Remove empty specimen slide holders from the discharge chute of the transfer station. Switch the instrument off and on. Proceed further as described in Chapter 5.3.
8. Malfunctions and Troubleshooting 8.2 Troubleshooting Problem Possible cause Remedy Air bubbles (between Air bubbles have developed in the specimen and cover slip). coverslip mountant from filling the coverslip mountant bottle. Observe the coverslip mountant's idle time of approx. 6 to 12 hours after filling the mountant bottle. Make sure that the coverslip mountant bottle is filled carefully.
8. Malfunctions and Troubleshooting 8.2 Troubleshooting (continued) Problem Possible cause Remedy Air bubbles (between The dispenser needle diameter has The size of the dispenser needle has to be selected specimen and cover slip). not been selected appropriately for according to the list of recommendations (refer to the coverslip mountant used. Chapter 5.12) of the various coverslip mountants or be determined as described in the configuration recommendations.
8. Malfunctions and Troubleshooting 8.2 Troubleshooting (continued) Problem Possible cause Remedy The specimen gets dam- The height of the dispenser needle The height of the dispenser needle is set too low, so that aged when coverslip is set incorrectly or the dispenser it scratches the specimen when coverslip mountant is mountant is applied. needle was inserted incorrectly. being applied. The height of the dispenser needle has to be readjusted to the correct height (refer to Chapter 4.7).
8. Malfunctions and Troubleshooting 8.2 Troubleshooting (continued) Problem Possible cause Remedy The display shows the 1. Low-quality specimen slides 1. Use specimen slides that have been manufactured in "CHECK SLIDES" message were used. accordance with the standard ISO 8037/1. and no specimen slides 2. Specimen slides or specimen 2. Check specimen slides and specimen slide holders for are gripped. slide holders have been damaged damage and replace them if necessary.
9. Optional Accessories 9.1 Ordering information To prevent damage to the instrument or specimens, only accessories and spare parts authorized by Leica may be used. Designation Order No.
9. Optional Accessories 9.1 Ordering information (continued): Designation Order No. Specimen slide holder 30, plastic, 5 pieces 14 0475 33643 Specimen slide holder 30, plastic, 1 piece 14 0475 33750 Specimen slide holder 30, mod.
9. Optional Accessories 9.1 Ordering information (continued): Leica CV Mount Coverslipping mountant for all common coverslipping machines and manual coverslipping. 4 bottles, 250 ml ea., in carton Order No. 14 0464 30011 Fig. 51 Leica ST Ultra Leica ST Ultra intermedium was developed as a xylene substitute for histological specimen mounting, removal of paraffin from specimens, and manual as well as automated specimen coverslipping.
9. Optional Accessories 9.1 Ordering information (continued): Standard active carbon filter, for working with xylene Order No. 14 0422 30673 Fig. 54 Fig. 55 Cover slips, of pure white glass of hydrolytic class 1, thickness No. 1 (0.13 – 0.17 mm) Supply quantity: 1000 pieces – in plastic boxes, each with 100 pieces Size 24x40 mm Order No. 14 0711 35635 Size 24x50 mm Order No. 14 0711 35636 Size 24x55 mm Order No. 14 0711 35637 Size 24x60 mm Order No.
9. Optional Accessories 9.1 Ordering information (continued): 7 10 1 3 8 9 4 5 6 2 Fig. 57 Dispenser needle cleaner, assembly Dispenser needle cleaner consisting of: • 1 container (1) with lid (10) • 2 brushes (2) • 1 holder (3) with hexagon socket screw (7) and shim (8) • 1 mounting bracket (4) with 2 hexagon socket screws (9) • 1 hexagon spanner (6) • 1 plastic pipette (7.7 ml) (1) • 1 set of Instructions for Use Order No.
9. Optional Accessories 9.1 Ordering information (continued): Cover slip catch tray Order No. 14 0478 39585 Fig. 60 Output magazine 30, for 30 specimen slides, 4 pcs. Order No. 14 0478 39586 Fig. 61 Output magazine 20, for 20 specimen slides, 6 pcs. Order No. 14 0478 40117 Fig. 62 Loading bath for specimen slide holder, flat Order No. 14 0478 39592 Fig. 63 100 Instructions for Use V 3.
9. Optional Accessories 9.1 Ordering information (continued): Loading bath for specimen slide holder, deep Unlike the flat loading bath, the deep loading bath has two notches on each side, which enable reliable differentiation. Order No. 14 0478 39657 Fig. 64 Cover for loading bath Order No. 14 0478 39584 Fig. 65 Fig. 66 Cover with cutout, for loading bath, only together with bath insert for Leica specimen slide holder for 30 specimen slides - 14 0478 39593 Order No.
9. Optional Accessories 9.1 Ordering information (continued): Cover slip magazine, coverslip magazine Multi-sizeTM with inserts for holding various common coverslip sizes 40-60x22 mm 40-60x24 mm Order No. 14 0478 39748 Order No. 14 0478 39749 Fig. 68 Exhaust hose, solvent-resistant, flexible, 3 m length, diameter 32 mm Order No. 14 0478 39820 Fig. 69 Glass vial with cover Order No. 14 0478 39789 Fig. 70 Suction cups, Pack of 2 Fig. 71 102 Order No. 14 0478 39701 Instructions for Use V 3.
9. Optional Accessories 9.1 Ordering information (continued): Sakura specimen slide holder, Type: Sakura plastic, 1 piece Note: when used together with ST4040, use transport clip 14 0474 34969. Order No. 14 0474 33463 Fig. 72 Specimen slide holder 30, Plastic, pack of 5 Order No. 14 0475 33643 Plastic - 1 pc. Order No. 14 0475 33750 Fig.
9. Optional Accessories 9.1 Ordering information (continued): Specimen slide holder 30, Modified, plastic, 1 piece, for Varistain adapter (14 0464 37659) Order No. 14 0478 38029 Fig. 74 Varistain adapter, Adapter for Shandon Varistain 24-4, Note: For use with Leica specimen slide holder 30, modified, plastic 14 0478 38029, to combine Varistain 24-4 with CV5030. Order No. 14 0464 37659 Fig. 75 104 Instructions for Use V 3.
9. Optional Accessories 9.1 Ordering information (continued): Bath insert Leica 20, for Leica specimen slide holders, for 20 specimen slides Order No. 14 0478 36706 Fig. 76 Bath insert Sakura 20, specimen slide holders for 20 slides Order No. 14 0478 36707 Fig. 77 Bath insert Shandon 20, for Shandon Gemini, specimen slide holder for 20 specimen slides Order No. 14 0478 36709 Fig. 78 O-ring for mountant bottle, 28x3 mm, pack of 5 Order No. 14 0253 45452 Fig.
10. Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/ or characteristics warranted. The scope of this warranty is determined by the contents of the contract that has been concluded.
Appendix A Application-related notes and recommendations A1.1 Leica specimen slide holders, output and coverslip magazines made of plastic Leica plastic accessories are manufactured from special material and designed for long-term use. Various factors, such as material-dependent aging, material fatigue conditioned by use, heat and dirt can lead to the changes in the plastic.
Appendix A Application-related notes and recommendations A1.1 Leica specimen slide holders, output and coverslip magazines made of plastic (continued) For the specimen slide holders to function flawlessly, avoid the following: • The specimen slide holders must be handled carefully. Do not let them fall or collide with other objects, which could result in fracture points that can impair the function of the specimen slide holders.
Appendix A Application-related notes and recommendations A1.3 Specimen slides and gripper mechanism The new gripper mechanism and the new specimen slide gripper can be identified by the red anodized gripper fingers. This change applies beginning with serial number 3000 for the CV5030. Notice! The gripper mechanism for the specimen slides is never allowed to be changed or manipulated during necessary cleaning measures within the instrument.
Appendix A Application-related notes and recommendations A1.4 CV5030 – Validated and recommended specimen slides (continued) VCE Micro-Slides Leica - Surgipath Ground edges 90° Name Manufacturer Properties and description Bloodsmear Slides / Art. 3010-SBE Leica - Surgipath Frosted End Beveled edges Bloodsmear Slides / Art.
Appendix A Application-related notes and recommendations The following specimen slides were validated for the specimen slide gripper starting with serial number 3000 (continued): Name Manufacturer Properties and description Colorfrost Plus Thermo Fisher Scientific Ground edges 90° Carl Roth GmbH Ground edges 45° Clipped corners Carl Roth GmbH Cut edges 90° VWR Cut edges 90° VWR Ground edges 45° Clipped corners A1.
Appendix A Application-related notes and recommendations A1.6 Labels for specimen slides The Universal Label labels are optimized for use on the CV5030 coverslipper and provide a complete solution for printing and coverslipping in combined usage. To ensure the best print quality, Leica Universal Label labels have to be used on the Cognitive Label printer. This is available exclusively from Leica Biosystems. Once printed and affixed to the specimen slides, the Leica Universal Label labels are durable.
