Biological Signals Amplifier www.wpiinc.com for isolated heart (Langendorff/Neely) and perfused organ systems INSTRUCTION MANUAL Serial No.
Biological Signals Amplifier CONTENTS ABOUT THIS MANUAL...................................................................................................................... 1 INTRODUCTION .................................................................................................................................. 1 Parts List............................................................................................................................................ 2 Unpacking...........................
iv World Precision Instruments
Biological Signals Amplifier ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—The Biological Signals Amplifier has four modules.
The amplifier contains four modules: two for recording fluid pressures (perfusion or aortic, pulse pressure or LVP), one for recording the temperature of the perfusate and tissue, and one for recording ECG signals or surface potentials. The main switch and fuses of the 220/110 V power supplies are located on the back panel of each rack. Input and output sockets are also found on the back panels. Operation switches and indicators are on the front panels.
Biological Signals Amplifier INSTRUMENT DESCRIPTION Fig. 2—The front panel has the operation switches and indicators. Fig. 3—The back panel has the input and output sockets, along with the main power switch.
Setting Up HG Pressure Amplifiers and Sensors The HG amplifier and its sensor are designed to measure the pressure of the fluid perfusing a heart or an organ (liver, kidney, mesentery), or the aortic pressure developed by the left ventricle of a heart in working mode. The second pressure amplifier and sensor in the system can be used with a catheter to measure diastolic and systolic pressures from the left ventricle of the heart. Fig. 4—(Right) The HG Pressure amplifier, front and back.
Biological Signals Amplifier NOTE: If the recorded output signals for the 10mmHg and 100mmHg settings are not 100mVDC and 1VDC respectively, recalibrate the pressure sensor to the HG1 module using the directions provided below. 6. Once the calibration of the sensor and amplifier have been verified, prepare to attach the pressure sensor to the cannula or catheter. Purge all lines to remove any bubbles in the perfusion fluid lines.
Setting up TH Thermometer Amplifier and Sensor The TH amplifier and its sensor are designed to measure the temperature of the fluid perfusing a heart or an organ (liver, kidney, mesentery) in order to determine the temperature of the organ. Fig. 6—The TH Temperature Amplifier, front and back. If the temperature sensor is going to be used: 1. Plug the microphone jack on the end of the temperature sensor to the TH IN socket on the back panel of the amplifier.
Biological Signals Amplifier Calibrating the Temperature Sensor to the TH Module 1. In an insulated container, prepare 2–3L of water or fluid at about 20ºC. In a second insulated container prepare 2–3L of water at a higher temperature, about 40ºC. 2. Put a precision thermometer (0.1ºC) through the lid of each insulated container so that the bulb of the thermometer is in the middle of the container. Leave an opening on the lid of each container for the temperature sensor. 3.
Setting up the ECG Amplifier The ECG amplifier is designed to record the ECG signals from as many as three different positions (leads I, II, III) on an animal using standard electrodes or surface potentials from the muscle fibers of an isolated heart using wire electrodes. This amplifier is appropriate for use on species in size from rats to dogs. The amplifier also has an internal rate meter which allows the heart rate to be recorded as ECG signals or surface potentials are also recorded.
Biological Signals Amplifier 3. Turn the 3-way program switch on the front panel of ECG amplifier to GND. Turn on the amplifier. A signal of zero (0) volts is sent by the amplifier to the data recorder. If necessary, set the baseline of the recording channel to zero (0) using the controls of the data recording system. 4. Check the calibration of the recording channel. Turn the 3-way program switch to 1mV.
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Biological Signals Amplifier WARRANTY WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including its components and parts, shall be free from defects in material and workmanship for a period of one year* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A.
World Precision Instruments, Inc. USA International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com UK 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 • Fax: 030-6188670 • E-mail: wpide@wpi-europe.com China & Hong Kong WPI Shanghai Trading Co., Ltd. Rm 20a, No8 Dong Fang Rd.
