SI-MT/SI-MKB/SI-HTB www.wpiinc.com Signal Conditioning Amplifier System for Muscle Testers INSTRUCTION MANUAL Serial No.
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SI-MT/SI-MKB/SI-HTB CONTENTS ABOUT THIS MANUAL ............................................................................................................................... 1 INTRODUCTION ........................................................................................................................................... 2 Features ......................................................................................................................................................
SI-H Muscle Testers Setting the Tissue Distance ..........................................................................................................31 Adjusting the CCD Sensitivity ......................................................................................................31 Setting the Camera (CCD) Mode ................................................................................................32 Aligning the Laser and the Camera ........................................................
SI-MT/SI-MKB/SI-HTB ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig.
SI-H Muscle Testers Fig. 3 The Signal Conditioning Amplifier System is a flexible chassis that is specifically configured for an SI-MT, SI-HTB or SI-MKB system (Muscle Testers). INTRODUCTION The Signal Conditioning Amplifier System provides a flexible electronic platform intended to process the transduction of mechanical signals, the filtering of transducer outputs and the control of motor positions.
SI-MT/SI-MKB/SI-HTB Features This Signal Conditioning Amplifier System offers eight expansion slots, configured at the factory to meet your requirements. NOTE: The system is configured at the factory. If you need to add additional modules, contact Technical Support at 941.371.1003 or TechnicalSupport@wpiinc.com. Cautions and Warnings WARNING: TURN OFF THE SIGNAL CONDITIONING AMPLIFIER SYSTEM AND UNPLUG IT FROM THE POWER OUTLET BEFORE REMOVING OR INSTALLING ANY MODULE IN THE UNIT.
SI-H Muscle Testers INSTRUMENT DESCRIPTION Signal Conditioning Amplifier for the SI-MT/SI-MKB/SIHTB Front Panel Optical Anti Transducer Oscillation Amplifier Unit Sarcomere Spacing Module Temperature Control Module Linear Motor Controller Power Switch Fig. 4 The front panel of a Signal Conditioning Amplifier System configured for a Muscle Tester shows the SI-BAM21-LCB, the SI-AOSUB, The SI-SARCAM, the SI-MOTDB and the SI-TCMB2.
SI-MT/SI-MKB/SI-HTB Temperature Control Module–When temperature control is required, the SI-TCM2 is used. It can control two cuvettes simultaneously, using digital control to maintain a constant temperature. It has both high and low alarm warnings which can be user defined. Constant Load Module–(Not pictured in the unit here.) It is often important to maintain a constant sarcomere length rather than keep the total length of the preparation constant during an isometric contraction.
SI-H Muscle Testers NOTE: The 16 plugs marked with A or B are for future development. They are not used at this time. SI-BAM21-LCB The SI-BAM21-LCB KG Optical Force Transducer Amplifier is used in conjunction with the SI-H tissue bath and muscle physiology systems. The SI-BAM21-LCB powers the force transducer and converts the output of the transducer to an amplified analog voltage that is proportional to the force applied to the force transducer.
SI-MT/SI-MKB/SI-HTB Front Panel Offset Adjustment LEDs Offset Adjustment Switch Zeroing LED Zero Button Gain Indicator LEDs Gain Switch Data Acquistion Output Force Transducer Connection Digital Interface Connection Gain Calibration Potentiometer Fig. 6 SI-BAM21-LCB KG Optical Force Transducer Amplifier Zero Button–When pressed, the SI-BAM21-LCB output comes close to zero and the Zeroing LED illuminates.
SI-H Muscle Testers Data Acquisition Output–Connect a data acquisition system like WPI’s Lab-Trax-8/16 to this BNC connector to record the raw SI-BAM21-LCB voltage output. For test purposes, a multi-meter or oscilloscope may be connected using a standard BNC cable (WPI #2851). Force Transducer Connection–An SI-KG force transducer is plugged into this DIN connector. Align the pins, and insert the connector until it is fully seated.
SI-MT/SI-MKB/SI-HTB Pulser Port–Connect the Pulser cable to this port when you need to calibrate the system for a force transducer. The force transducer fits inside the Pulser, and the Pulser uses a strong electro-magnet to exert small square-wave forces on the force transducer. Pulser Amplitude Adjustment Knob–When calibrating a force transducer, this knob adjusts the amplitude of the pulser waveform so the display registers on the Signal Amplitude Array.
SI-H Muscle Testers A red laser diode (=650nm) is used. A one-dimensional (linear) CCD camera with a time resolution of 1ms scans the light intensity of the diffraction pattern beginning 6.8mm from the center beam. The camera scans only half the diffraction pattern, assuming that the pattern is symmetrical with respect to the center beam.
SI-MT/SI-MKB/SI-HTB the shorter sarcomeres superimposed on the lines of the longer sarcomeres. This provides a qualitative method for judging how much the sarcomeres get disordered during a contraction. Broadening of the sarcomere distribution indicates increased sarcomere disarray. Often this is not reversible and indicates loss of sarcomere integrity and reduced viability of the muscle preparation.
SI-H Muscle Testers LCD Display–Upon startup, this display shows the version of the software the SI-SARCAM is running. During normal operations, this display can show the calculated (actual) sarcomere spacing, the first order diffraction distance or the signal amplitude. During configuration, this display shows parameters and confirmation messages. Laser Intensity–Rotate this dial to change the intensity of the laser's power output.
SI-MT/SI-MKB/SI-HTB Fig. 14 The Linear Motor is an optional part for the SI-MT or SI-MKB systems. The position of the linear motor is determined by a combination of the data from the controller indicating the current position and the DC value applied to the front panel at the Position In port. The applied Position In signal can be provided by a data acquisition system. The data acquisition analog output signal is set to define the waveform and timing pattern of force to be applied to the sample.
SI-H Muscle Testers Front Panel Motor Connection Port Position In Port Position Out Port Power LED Fig. 15 The Linear Motor Controller Motor Connector–Use this port to connect the linear motor used with the SI-MT or SIMKB muscle testers. The motor is setup and calibrated to the Linear Motor Controller that is shipped with it. The motor and controller have a maximum range of motion of ±3.5mm from the center of travel. Position In–This BNC interface provides for an external position command input.
SI-MT/SI-MKB/SI-HTB SI-TCM2B The SI-H Temperature Control Unit is designed for use with the SI-H line of muscle physiology research platforms. It maintains the temperature of an SI-H cuvette up to 45ºC. This unit is available in a standalone model or as a module for the Signal Conditioning Amplifier System backplane.
SI-H Muscle Testers Configuration Buttons–The Display button is used to toggle the display between Channel 1 temperature, Channel 2 temperature and both. The Setup button rotates through the array of configurable parameters. The Up and Down buttons are used to adjust the parameters. Cuvette Connections–Use these ports to connect SI-H cuvettes used with the SI-MT and SI-MKB platforms. SI-COLUB The SI-COLUB Constant Load Module can be used in one of three different modes.
SI-MT/SI-MKB/SI-HTB In constant load experiments, the initial length of the muscle is set using the manual adjustments of the muscle research platform. While the muscle is held at its initial length, it is stimulated with tetanizing pulses so that the muscle develops its isometric force. These stimulus pulses are controlled by the stimulation protocol created in MDAC and sent from the analog stimulus output of the LabTrax 8/16.
SI-H Muscle Testers • Constant Load–This mode maintains a constant load on the tissue sample.
SI-MT/SI-MKB/SI-HTB the motor so that the sarcomere length is held constant while trying to study true isometric contractions • Bypass–In this mode, the Constant Load Module is completely bypassed. Bypass Motor Command received from LabTrax 8/16 Command sent to Linear Motor To use Bypass Mode, send a continuous voltage of at least 3.0V from one of the analog or digital outputs of the LabTrax 8/16 to the CL Bypass inputs of the SI-COLUB.
SI-H Muscle Testers Stretch Control Knob–This 12-position dial adjusts the response time of the stretch and slack periods of the tissue sample. The setting you choose depends on the sensitivity of your force transducer and the strength of your sample tissue. Force Step Dial–During a tonic contraction, activation of the SI-COLUB unit causes the force to drop to the level, which is set using with the Force Step dial.
SI-MT/SI-MKB/SI-HTB Connecting the Signal Conditioning Amplifier System Force Transducer 5-pin cable Linear Motor Data Acquisition Analog Input Data Acquisition Analog Input (Force recording channel) (Sarcomere channel) Laser Fig.
SI-H Muscle Testers • IF the SI-BAM21-LCB transducer amplifier module and the SI-AOSUB antioscillation module are not connected to each other through the backplane of the Signal Conditioning Amplifier System, these two modules must be connected through ports on the front panels of the modules. Use a BNC-BNC cable to connect the Force Output port on the front panel of the SI-BAM21-LCB module to the Input port on the front panel of the SI-AOSUB module.
SI-MT/SI-MKB/SI-HTB Constant Load Ext Cmd CL Bypass External Loop EXT CMD (toggle switch–up position) This connection is not used. IF desired, you can apply a 0.0V continuous signal or tie it to ground. Output (AO or DO) This connection is not from LabTrax 8/16 that used. IF desired, you can generates a continuous apply a 0.0V continuous high voltage of at least signal or tie it to ground. 3.0V Capture Control signal from one of the digital outputs of the LabTrax 8/16.
SI-H Muscle Testers OPERATING INSTRUCTIONS Turning the System On For convenience, the Signal Conditioning Amplifier System has two power switches, and both must be on to power the system. One is located on the back panel, and one is on the front. Both switches must be on to power the system. Verify that the power cord is properly installed and plugged into an AC power outlet. All the modules power on/off simultaneously. When the system is setup, just leave the back power switch in the on (I) position.
SI-MT/SI-MKB/SI-HTB 1. Setting a zero reference point with the force transducer un-loaded. 2. Applying a load with a known mass to the tissue mount on the transducer. 3. Choosing one of the two calibration methods to best serve the application. Use the Gain Calibration Potentiometer to adjust the amplifier’s output range to: • Maximize the resolution for the intended measurement range. For the greatest precision, maximize the resolution of the SI-BAM21-LCB by calibrating the 10.
SI-H Muscle Testers Force Transducer Assembly Tissue Mounting Hook Calibration Stand Fig. 21 When the force transducer is properly mounted in the calibration stand on the muscle tester, the force transducer is held at the same angle used when making measurements. 3. Set the Gain switch on the front panel of the SI-BAM21-LCB to the gain (X1-X10) that is suitable for the mass being used for the calibration of the selected transducer. 4.
SI-MT/SI-MKB/SI-HTB 8. If you intend to use multiple amplification factors, cross-check your calibration. For example, if a 0.5g mass was used to calibrate the SI-BAM21-LCB as close as possible to 10.0V at a gain of X10, then at a gain of X1, the monitor should display an output very close to 1.0V for the same 0.5g calibration mass. Making Measurements After the SI-BAM21-LCB has been calibrated, measurements may be taken. 1. Turn the SI-BAM21-LCB Power switch on (I).
SI-H Muscle Testers Fig. 22 (Left) This pulser assembly has no force transducer mounted in it. Fig. 23 (Right) A force transducer is mounted in the SI-AOSUB pulser assembly. Keep in mind that: • The closer the anti-oscillation frequency matches the resonance frequency of the force transducer, the more the ringing phenomenon is removed from the force signal. • The resonance frequency can be evoked at anti-oscillation frequencies that are multiples of the resonance frequency.
SI-MT/SI-MKB/SI-HTB 6. Continue to rotate the calibration screw of the Anti-oscillation Frequency Adjustment potentiometer to the right (clockwise) until the greatest number of bars on the Signal Amplitude LED array are illuminated. If the Signal Amplitude LED array becomes fully illuminated as the anti-oscillation frequency is increased, decrease the pulse amplitude by turning its control knob to the left (counterclockwise).
SI-H Muscle Testers 5. Press the Display button to save the configuration and return to the normal display. NOTE: The unit remembers the state of all the parameters, even after it is powered off. To reset the factory defaults, turn the unit off, press both the Up and Down buttons simultaneously while you turn the system back on. NOTE: For safety reasons, the laser is always disabled at startup.
SI-MT/SI-MKB/SI-HTB Adjusting the CCD Offset CAUTION: The CCD offset should not be modified from the factory default of 6.8mm unless you have a reason to place the zero order laser spot at a different location other than the cross hair mark on the cuvette. 1. Press the Setup button until "CCD Offset" appears. This is the distance between the crosshair marking on the cuvette and the first pixel of the CCD camera. CCD Offset 6.8 mm Fig. 27 Press the Up or Down button to adjust the offset. 2.
SI-H Muscle Testers Setting the Camera (CCD) Mode 1. Press the Setup button until “CCD Mode" displays. Two options are available: • If you set the system to 263Hz (default), the full display of the CCD is used and the camera operates at 263Hz. • For faster operations (1KHz), set the system to 1KHz. At the faster speed, only the first quarter of the CCD camera display is used for making measurements. CCD Mode 263 Hz Fig. 30 Press the Up or Down button to change the CCD mode.
SI-MT/SI-MKB/SI-HTB SI-SARCAM Front SI-SARCAM Back Camera CCD Detector Chip Cross Hair Mark Optical Cuvette AVOID III Laser diode 5mW Class MaxOut: aperatu from this emittedto the beam e m Laser radiation direct exposur th: 630–680n Avoid waveleng A Laser Product E EXPOSUR re Laser Laser X-Y Adustment Knob Camera X-Y Adustment Knob Camera Height Adustment Knob Laser Height Adustment Knob Fig. 32 The front and back of an SI-SARCAM system is shown mounted on an SI-MKB platform.
SI-H Muscle Testers Fig. 33 (Left) The sarcomere grating fits into the cuvette and provides a foolproof calibration method. Fig. 34 (Right) The grating is position in the optical cuvette to calibrate the camera. 1. Turn on the system. See "Turning the System On" on page 24. 2. Enable the Laser. For safety reasons, the laser is always disabled on startup. See "Turning On/Off the Laser" on page 30.
SI-MT/SI-MKB/SI-HTB 2. Adjust the trigger level on the oscilloscope. 3. Connect the Video BNC connector to the analog input of the oscilloscope. 4. Set the time scale of the oscilloscope to display the desired portion of the video signal. The scan of the photodiodes lasts 1ms in 1KHz mode or 3.8ms in 263Hz mode. NOTE: If the scanned signal is less than 1.0V, the sarcomere length may be incorrectly calculated.
SI-H Muscle Testers Heating Indicator–A flashing arrow pointing up (↑) indicates that the cuvette is heating. Setup 1. Turn on the system. 2. Line up the cuvette connector with the port on the SI-TCM2, press it into place and screw the outer ring of the connector to secure the connector. 3. Press the Setup button to toggle through the setup parameters. 4. Press the Display button to save the configuration and return to the normal display.
SI-MT/SI-MKB/SI-HTB CH 1 Setpoint 37.0º C Fig. 39 Press the Up and Down buttons to adjust the Channel 1 Setpoint. 2. Press the Up or Down button to adjust the setpoint. The maximum setpoint allowed is 45ºC. 3. Press the Display button to save the configuration and return to the normal display. Setting Alarms Both Channel 1 and Channel 2 have high and low alarm values. By default, the low alarms are set at 36ºF, the high alarms are set at 38ºF and the alarms are disabled. 1.
SI-H Muscle Testers Enabling/Disabling the Alarms By default the alarms are disabled. When enabled, the unit will emit a beep when an alarm state occurs. 1. Press the Setup button until “CH1 Alarm” or “CH2 Alarm” appears on the screen. CH1 Alarm DISABLED Fig. 42 By default the alarms are disabled. 2. Press the Up or Down button to enable or disable the alarm. 3. Press the Display button to save the configuration and return to the normal display.
SI-MT/SI-MKB/SI-HTB MAINTENANCE The Signal Conditioning Amplifier System is maintenance free. However, to protect it, follow these guidelines: • Place the Signal Conditioning Amplifier System in a clean, dry location. • Keep liquids away from the Signal Conditioning Amplifier System connections.
SI-H Muscle Testers TROUBLESHOOTING Resonance noise still exists on the transducer output signal SI-BAM21-LCB has no output signal (0.0V DC) Chassis has no power Issue Possible Cause Solution One of the two power switches is off. Verify that the power switch one the back of the chassis and the power switch on the front panel are both in the on (I) position.
SI-MT/SI-MKB/SI-HTB SPECIFICATIONS This instrument conforms to the following specifications: Chassis Maximum Power Consumption 1.3A at 115V 50/60Hz, 1.
SI-H Muscle Testers 42 WORLD PRECISION IN STRU MENTS
SI-MT/SI-MKB/SI-HTB INDEX expansion slots 5 F 500216-G ii 500217 ii 500217-G ii feedback 11 filter 8, 27 force transducer 6, 7, 8, 21, 40 fuse 5 A G alarm 15, 37 amplifier 6 amplitude 9 analog voltage 6 anti-oscillation 21, 27 anti-oscillation unit 4, 8 gain 7 gain factor 6 grating 9, 10, 33, 34 Symbols B backlight level 37 backplane 5 baseline 6, 7 Biofluorometer ii BNC 22, 40 buffer 6 C calibration potentiometer 8 camera 10, 12, 29, 33 CCD camera 10, 12 CCD Mode 32 CCD offset 31 CCD Sensitivity
SI-H Muscle Testers WARRANTY WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including its components and parts, shall be free from defects in material and workmanship for a period of one year* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A.
