Thermo Scientific Multidrop Combi User Manual Rev. 1.
Thermo Scientific Multidrop Combi User Manual Rev. 1.5, Cat. no.
Copyright © 2009 Thermo Fisher Scientific Corporation. All rights reserved. First edition published in 2005. Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is prohibited. “FILLit” is a trademark of Thermo Fisher Scientific. “Immulon”, “Microfluor”, “Microtiter” and “Multidrop” are registered trademarks of Thermo Fisher Scientific. “Microlite” is a trademark of Thermo Fisher Scientific.
About This User Manual Intended users How to use this user manual This user manual is written for the actual end user (for example, laboratory technician) and provides information on the Thermo Scientific Multidrop Combi, including the installation and operating instructions.
About This User Manual Safety symbols and markings Safety symbols and markings Safety symbols and markings used on the Multidrop Combi These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated. The following symbols and markings appear on the type label and the instrument itself.
About This User Manual Safety symbols and markings Warning and other markings used in the documentation The following symbols and markings appear in this user manual. Warning Risk of electric shock. ▲ Warning Biohazard risk. ▲ Warning Risk of injury to the user(s). ▲ Caution Risk of damage to the instrument, other equipment or loss of performance or function in a specific application. ▲ Note Marks a hint, important information that is useful in the optimum operation of the system, or an item of interest.
About This User Manual Instrument safety and guidelines for use Instrument safety and guidelines for use 6 Thermo Scientific Multidrop Combi User Manual ● Always follow basic safety precautions when using the Multidrop Combi to reduce the risk of injury, biohazardous contamination, fire, or electrical shock. ● Read this user manual in its entirety prior to operating the instrument.
Contents Intended users ................................................................................... 3 How to use this user manual ............................................................. 3 For more information ....................................................................... 3 Safety symbols and markings............................................................. 4 Safety symbols and markings used on the Multidrop Combi.......... 4 Warning and other markings used in the documentation ...
Contents Dispensing cassette and volume.................................................... 40 Column selection ......................................................................... 41 Protocol settings.............................................................................. 44 Dispensing speed.......................................................................... 45 Dispensing offset.......................................................................... 46 Dispensing height ................
Contents Materials and equipment required for precision verification ...... 85 Precision verification procedure................................................. 85 Calibration (gravimetric).............................................................. 87 Material and equipment required for calibration ....................... 87 Calibration procedure ............................................................... 87 Calibration (standard tube volumetric).........................................
Figures Figures Figure 1–1. Multidrop Combi microplate dispenser......................................14 Figure 2–2. Multidrop Combi front view........................................................17 Figure 2–3. Close-up of the Multidrop Combi keyboard and display............18 Figure 2–4. Multidrop Combi back view........................................................18 Figure 3–5. Transport lock and transport lock tag present ...........................21 Figure 3–6. Transport lock fastened ..........
Figures Figure 5–36. Pressing the tubing identification plate into place.................. 75 Figure 5–37. Pressing the tube fasteners into place .................................... 76 Figure 5–38. Fastening the calibration screws ............................................. 76 Figure 5–39. Calibration screws fastened .................................................... 76 Figure 5–40. Replacing the cover of the upper part of the dispensing cassette .......................................................
Tables Tables Table 4–1. Main types of Thermo Scientific Multidrop Combi dispensing cassettes......................................................................................26 Table 4–2. Icons in the main view .................................................................35 Table 4–3. Default parameter values for different plate types ....................39 Table 4–4. Dispensing volumes of the cassettes..........................................40 Table 4–5. Dispensing speeds ......................
Chapter 1 Introduction to the Multidrop® Combi The available models of the Multidrop Combi are: ● 5840300 Multidrop Combi, 100 – 240 V 50/60 Hz ● 5840310 Multidrop Combi with SMART option, 100 – 240 V 50/60 Hz Each Multidrop Combi comes with a sample of different dispensing cassettes, including both small and standard tube cassettes. The included cassettes can cover the whole volume area, which then immediately enables full use of the instrument.
Introduction to the Multidrop® Combi Principle of operation The Multidrop Combi uses detachable and autoclavable dispensing cassettes. The dispensing cassette contains eight individual tubes. Each reagent can have a separate dispensing cassette to avoid mixing of reagents. Alternatively, the same dispensing cassette can be used with several reagents if the dispensing cassette is washed in between use. Refer to “Dispensing cassettes” on page 25.
Introduction to the Multidrop® Combi Advantages of using Multidrop Combi Advantages of using Multidrop Combi Thermo Fisher Scientific The Multidrop Combi microplate dispenser provides several advantages relating mainly to the principle of operation in that it has: ● Flexible volume range of 0.
Introduction to the Multidrop® Combi Advantages of using Multidrop Combi 16 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Chapter 2 Functional Description Instrument layout Front view This section shows the front and back views of the Multidrop Combi instrument. The front view of the Multidrop Combi instrument is shown in Figure 2–2. Display Rotor cover Keyboard Pump body Priming vessel Plate carrier Figure 2–2.
Functional Description Instrument layout The close-up of the Multidrop Combi keyboard and display is shown in Figure 2–3. Display Keyboard Figure 2–3. Close-up of the Multidrop Combi keyboard and display Back view The back view of the Multidrop Combi instrument is shown in Figure 2–4. WEEE symbol Mains connector Mains switch Instrument label Serial connector USB Figure 2–4.
Chapter 3 Installation Delivery check This section covers the relevant procedures to be carried out on receipt of the instrument. Checking delivery Check the enclosed packing list against order. In case of any deviations, contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy. Visually inspect the transport package, the instrument and the accessories for any possible transport damage.
Installation Requirements Requirements When you set up your Multidrop Combi, avoid sites of operation with excess dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive moisture or large temperature fluctuations. ● Make sure the working area is flat, dry, clean and vibration-proof and leave additional room for accessories, cables, reagent bottles, and so on. ● Make sure the ambient air is clean and free of corrosive vapors, smoke and dust.
Installation Installation setups Installation setups This section describes the installation setups that you must carry out before operating or relocating the instrument. Warning Ensure that the mains switch (Figure 2–4) at the left of the back panel is in the OFF position. Also ensure that the mains supply cable is disconnected. ▲ Releasing the transport lock The instrument has a transport lock (Figure 3–5). Make sure the red transport lock is released before operating the instrument. Figure 3–5.
Installation Installation setups 2. Remove the screws, the transport lock piece and the transport lock tag from the screw marked 1 (Figure 3–6). Keep the tag for future relocation or transportation of the instrument. The transport lock is now released. 3. After that fasten the red transport lock piece to the back panel of the dispenser with the same screws for storage (Figure 3–7). Keep the transport lock there until needed for future relocation or transportation of the instrument. Figure 3–7.
Installation Operational check Operational check Complete the following procedure without the dispensing cassette to confirm the correct functioning of the dispenser prior to normal use. 1. Connect the mains supply cable and switch the instrument on using the mains switch (Figure 2–4). ● If the instrument starts properly: ● The display lights up. ● The plate carrier moves to the home position and the pump lifting mechanism to the up position. 2.
Installation Operational check 24 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Chapter 4 Routine Operation Priming vessel Ensure that the priming vessel is inserted correctly into its slot on the left of the plate carrier. You have fastened it correctly when you slide it over a snap lock. Make sure you have a tube assembly or a cap inserted into the drain of the priming vessel (Figure 4–9) or a vessel underneath the drain.
Routine Operation Dispensing cassettes Table 4–1. Main types of Thermo Scientific Multidrop Combi dispensing cassettes Picture Code Item Dispensing range Reagent pretreatment Tubing material and ∅ Tip material and inner ∅ Autoclavability 24073290* Small tube plastic tip dispensing cassette, blue With SMART option 0.5-50 µl preferably filtered, < 50 µm particles silicone 0.4 mm PP 0.22 mm 10 times Small tube metal tip dispensing cassette, gray With SMART option 0.
Routine Operation Dispensing cassettes Installing the cassette 1. Take the lower part of the cassette with the tip protection (only in small tube cassettes) into your right hand with the dispensing cassette tips pointing down and the upper part into your left hand (Figure 4–11). Figure 4–11. Inserting the dispensing cassette 2. Carefully place the eight tubes below the pump rotor and insert the lower part of the dispensing cassette into the lower part slots of the pump body (Figure 4–12).
Routine Operation Dispensing cassettes Figure 4–13. Inserting the upper part of the dispensing cassette Note The tension limiting wires are designed to prevent the user from pulling the tubes too much as excessive tension may damage the tubes or change the calibration of the dispensing cassette. ▲ 4. Double-check that both the upper and the lower parts of the dispensing cassette are properly placed into their corresponding slots (Figure 4–13 and Figure 4–14). Upper part slots Figure 4–14.
Routine Operation Dispensing cassettes Figure 4–15. Correct placement of all the dispensing cassette tubes 6. Ensure that the tension limiting wires have a loose fit around the rotor shaft. 7. With small tube cassettes, pull the tip protection off from the dispensing cassette once the dispensing cassette has been installed (Figure 4–16). 8. Remove the tubing weight protection by removing the rubber holder and pulling the tubing weight out of the tubing protection tube (Figure 4–17 and Figure 4–18).
Routine Operation Dispensing cassettes Figure 4–18. Cassette with the tip and tubing weight protections removed 9. Place the tubing weight into the reagent vessel, and always ensure that there is sufficient liquid present to run the protocol. During prolonged standby, keep the cassette in its rest position (Figure 4–19). Figure 4–19. Rest position of the dispensing cassette 10. Pull the rotor cover over the rotor (Figure 4–20). A sensor controls that the cover is properly placed over the pump rotor.
Routine Operation Dispensing cassettes Figure 4–20.
Routine Operation Control panel Control panel Keyboard This section describes the Multidrop Combi control panel and internal software. The keyboard is shown in Figure 4–21. Control buttons Arrow keys and OK button Figure 4–21. Keyboard of the Multidrop Combi Keys The relevant keys and control buttons are described in detail below. The arrow keys are used to select, modify and change the parameters and to navigate in the main view. The OK button is used to accept the selection.
Routine Operation Control panel Display The main view in the display is shown in Figure 4–22. Menu tabs Icon Main view row Info text bar Figure 4–22. Main view on the display of the Multidrop Combi There are three menu tabs in the Multidrop Combi user interface: Main, Settings and Options. In routine use you mainly navigate in the Main menu. In advanced level options there are two extra levels, Settings and Options. You can navigate between these three menu levels using the Left and Right arrow keys.
Routine Operation Control panel Navigating This section visualizes navigation in the Multidrop Combi user interface. The main view changes according to the selections you make either with the Up and Down arrow keys or the OK button. The available buttons and their function are shown on the info text bar. Active item Available buttons and their function The color of the items, for example, the icon and main view row, in the main view changes when they are selected (active/inactive).
Routine Operation Control panel Table 4–2.
Routine Operation Dispensing Dispensing You can immediately start dispensing by following the quick start guidelines below. Routine dispensing only requires to key in a few buttons. 1. Power ON the instrument. 2. Insert the correct dispensing cassette and the priming vessel. Close the rotor cover. 3. Make sure you are in the Main menu. 4. Select the plate type and insert the plate. 5. First select the cassette type and then the dispensing volume. 6.
Routine Operation Three key selections to start dispensing After the instrument is turned on, make the following selections to start dispensing when you are in the Main menu: plate type, dispensing volume and cassette, and number of columns. For more information, refer to “Dispensing parameters” on page 38. 1 – Plate type; see Step 3 on p. 36.
Routine Operation Using internal software Using internal software Dispensing parameters This section describes procedures related to the Multidrop Combi internal software. Multidrop Combi can also be controlled with PC software, Thermo Scientific FILLit Software for Multidrop Combi (Cat. no. 5188010). For more information, refer to Thermo Scientific FILLit Software for Multidrop Combi User Manual (Cat. no. N07173).
Routine Operation Dispensing parameters Select the plate type using the Up and Down arrow keys. Press OK to accept the selection. The available plate types and the default parameter values are given in Table 4–3. Each plate type has a selected default dispensing cassette type, which is automatically set by the instrument. Table 4–3. Default parameter values for different plate types Plate type Cassette type Max.
Routine Operation Dispensing parameters Dispensing cassette and volume There are two types of dispensing cassettes available, Small tube cassette (either plastic tip or metal tip) and Standard tube cassette. Refer to “Dispensing cassettes” on page 25 and Table 10–15. Each dispensing cassette type covers a specific volume area. Go to the Main menu. Select the Volume and cassette row. Press OK. The default cassette type and dispensing volume for the selected plate type are shown. Max. volume Min.
Routine Operation Dispensing parameters The default cassette types and dispensing volumes for different plate types are given in Table 4–3. Accept the selections using the OK button. Ensure that the cassette selection corresponds to the installed cassette. Column selection You can dispense either into the whole plate or only into certain selected columns. Go to the Main menu. Select the Column selection row. Press OK.
Routine Operation Dispensing parameters Column number Use the arrow keys to move from one column to the next. Press the OK button every time to select a column. The selected column becomes red. Use the OK button to toggle between a filled or empty column. Use the Down arrow key and select Accept and press OK after selecting all the columns you wish. You can press the STOP button to exit the column selection without making any changes.
Routine Operation Dispensing parameters Volume value box Press the OK button for 0.5 seconds. The Volume value box is now activated (bright red). Adjust the dispensing volume of the column where the cursor is located using the Up and Down arrow keys. Press OK after selecting the desired volume. You can press the STOP button to exit the volume selection without making any changes.
Routine Operation Protocol settings Protocol settings This section describes the additional parameters that supplement the plate type you have selected. These additional parameters are set in the Settings menu. The values shown in the Settings menu are those set for the plate type that is selected in the Main menu. Note The protocol settings do not normally have to be changed in routine use.
Routine Operation Protocol settings Dispensing speed The default dispensing speed is High, but you can change the dispensing speed, for example, to reduce foaming. Note that the Medium dispensing speed gives the best dispensing results with most of the buffers. Go to the Settings menu. Select the Pump speed row. Press OK. Use the Up and Down arrow keys to select the pump speed. The available settings are High, Med (medium) and Low. The default speed is High. Press OK. Table 4–5.
Routine Operation Protocol settings Dispensing offset The default dispensing offset is 0.0/0.0 mm. You can change the x- and y-axis offset values if the plate used is not the standard one specified in the plate type list (see Table 4–3). Go to the Settings menu. Select the Dispensing offset row. Press OK. The track mechanism and pump body move to the setting position.
Routine Operation Protocol settings Dispensing height The default dispensing height depends on the plate type (see Table 4–3). You can change the dispensing height values if the plate used is not the standard one specified in the plate type list. Go to the Settings menu. Select the Dispensing height row. Press OK. The track mechanism and pump body move to the setting position. Use the Up and Down arrow keys to set the dispensing height. While you change the height, the pump body moves accordingly.
Routine Operation Protocol settings Predispensing volume You can change the predispensing volume – the volume that is automatically dispensed before the start of plate filling, if necessary. Some more viscose solutions used may sometimes require extra predispensing. Go to the Settings menu. Select the Predispense (Prime) row. Press OK. Use the Up and Down arrow keys to set the predispensing volume.
Routine Operation Protocol settings Dispensing direction The row wise dispensing direction is default for each plate type. However, you may change the dispensing direction, if necessary. Go to the Settings menu. Select the Dispensing direction row. Press OK. Row wise direction Column wise direction Use the Up and Down arrow keys to set the dispensing direction, row wise or column wise. Note that the whole action is only effective when 384 or 1536-well plate types are selected. Press OK.
Routine Operation Running a protocol With the 1536-well plate selection, the columns with 32 wells are filled in four phases: first every fourth row (A, E, I, M … AC) and then the pump carrier shifts sideways above the rows (B, F, J, N … AD), which are then filled in the opposite order. After three shifts the remaining rows (D, H, L, P … AF) are filled.
Routine Operation Running a protocol Save and edit protocol Go to the Main menu. Select the Save and Edit Protocol row. Press OK. Press the Right arrow key to name/edit the name of your protocol. Enter the name of your protocol. Use the arrow keys to choose the character you want. Press OK to enter the said character.
Routine Operation Running a protocol ... , The available characters are: a to z, 0 to 9, hyphen (-), underline (_), , and space. ... , , Note Use max. 20 characters, including the space character. Some of the characters are broader than the others, whereby some of the names you edit will display less than 20 characters in the protocol list, shown as three dots at the displayed name. The protocol name is automatically capitalized. ▲ Backspace button To remove characters, use the Backspace button.
Routine Operation Running a protocol The sandglass window will appear while the Save action is in progress. The protocol name is now shown on the Save and Edit Protocol row. You can create a maximum of 100 protocols, including the factory defined protocols.
Routine Operation Running a protocol Select protocol To select an existing protocol for a particular plate type, first select the plate type (see “Plate type” on page 38) and then select the Save and Edit Protocol row. The protocols are listed according to each plate type. Press OK. Select the protocol using the Up and Down arrow keys. Press OK to accept the selection. The protocol is now selected. Note In the protocol list only the protocols made for the plate type in question are shown.
Routine Operation Running a protocol Press OK. Select the protocol using the Up and Down arrow keys. Press the Right arrow key to enter the edit mode. Delete button To delete the protocol, use the Delete button. Use the Down arrow key (and if needed, the Left and Right arrow keys) to select the Delete button and then the OK button to delete the protocol. You cannot delete default protocols (the Delete button is not activated).
Routine Operation Instrument options Instrument options This section describes the instrument parameters. All these parameters are set in the Options menu. The values shown in the Options menu remain in the instrument memory and are instrument specific, not protocol specific. Note You do not normally have to change the instrument options in routine use. ▲ Computer interface There are two types of computer interfaces available: RS-232 and USB. Go to the Options menu. Select the Computer interface row.
Routine Operation Instrument options Remote mode The Multidrop Combi can emulate the Thermo Scientific Multidrop 384 and Thermo Scientific Multidrop Micro command sets when in remote use. Thus it enables replacement of an old Multidrop unit with a Multidrop Combi. Select the dispenser in remote mode in question. Refer to “Remote control to Multidrop Combi” on page 98. Go to the Options menu. Select the Remote mode row. Press OK. Use the Up and Down arrow keys to select the remote mode in use. Press OK.
Routine Operation Instrument options Start-up protocol You can set which protocol is automatically selected in the Main menu when the Multidrop Combi is powered on. Go to the Options menu. Select the Start-up protocol row. Press OK. Use the Up and Down arrow keys to select the start-up protocol. The protocols are listed grouped and indented under each plate type. Press OK. The protocol name is appended with the plate type.
Routine Operation Instrument options When the instrument is started up the next time, the plate type and the protocol are those just selected. Buzzer You can choose whether or not the instrument produces a tone for different functions. Note that all functions make a certain sound. Go to the Options menu. Select the Buzzer (Instrument settings) row. Press OK. Use the Left and Right arrow keys to select whether the buzzer (audible tone) is On or Off. Press OK.
Routine Operation Instrument options Cassette calibration It is possible to calibrate the cassettes for different fluids. There are two different calibration modes available: Gravimetric and Standard tube volumetric (see “Verifying and recalibrating the dispensing cassette” on page 81). Go to the Options menu. Select the Calibration row. Press OK. Use the Up and Down arrow keys to select the calibration mode. If a SMART cassette is used, the calibration data can be viewed. Press OK.
Routine Operation Instrument options Dispensing cassette with SMART option The SMART dispensing cassette has an RFID tag incorporated that contains the information when the cassette was last calibrated, the cassette type, how much the cassette has been used for dispensing and the total usage. Refer to antenna, RFID and RFID tag in the glossary. The Multidrop Combi equipped with SMART option calculates the usage of the cassette by incrementing rotations of the pump.
Routine Operation Screen saver Table 4–6.
Routine Operation Emergency situations 3. If there is a longer break (for example, a day), wash the dispensing cassette by priming it with deionized distilled water or with special washing detergent if necessary. 4. After washing and emptying, store the dispensing cassette in the rest position (Figure 4–19). 5. Switch the Multidrop Combi off by pressing the mains switch (Figure 2–4) at the left of the back panel of the instrument into the OFF position. 6.
Routine Operation Emergency situations 64 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Chapter 5 Maintenance A) Instrument maintenance Regular and preventive maintenance This section describes issues related to maintenance of the instrument. For reliable daily operation, keep the instrument free of dust and liquid spills. To prevent unnecessary wear or hazards, follow the routine and service procedures described below at the frequency with which they should be applied.
Maintenance Disposal of materials Disposal of materials Follow laboratory and country-specific procedures for biohazardous or radioactive waste disposal. Refer to local regulations for the disposal of infectious material. Warning The samples can be potentially infectious. Dispose of all used dispensing cassettes, plates, strips, priming vessels, disposable gloves, syringes, disposable tips, and so on as biohazardous waste.
Maintenance Decontamination procedure 2. Empty the reagent container. Ensure that you are wearing disposable gloves. 3. Switch OFF the power (Figure 2–4) and disconnect the mains supply cable. 4. Disinfect the outside of the instrument using a cloth dampened with 70% ethanol. 5. Place the dispensing cassette, for example, in 1 – 3% Virkon solution or 4% glutaraldehyde solution for 10 minutes and rinse it with deionized distilled water. The dispensing cassette can alternatively be autoclaved. 6.
Maintenance Packing for service Packing for service To pack for service, follow the guidelines presented below. Caution It is important that the instrument is thoroughly decontaminated before it is removed from the laboratory or any servicing is performed on it. ▲ When you ship the instrument for service, remember to: ● Inform about the use of hazardous materials. ● Decontaminate the instrument beforehand.
Maintenance B) Dispensing cassette maintenance Warning Decontaminate the instrument before disposal. Refer to “Decontamination procedure” on page 66 and “Certificate of Decontamination” on page 107 about decontamination. ▲ Follow laboratory and country-specific procedures for biohazardous or radioactive waste disposal. Dispose of the instrument according to the legislation stipulated by the local authorities concerning take-back of electronic equipment and waste.
Maintenance B) Dispensing cassette maintenance 5. If the instrument is not used for a couple of hours, insert the dispensing cassette into the rest position (Figure 4–19). Refer to “Shutdown” on page 62. Handle the dispensing cassette with great care so that you do not damage the tubes and dispensing tips. These factors affect the useful life of the dispensing cassette.
Maintenance Changing the tubing set 4. Attach the syringe with a round filter unit (provided) and then attach the pink adapter with the short piece of tubing (provided) onto the filter unit. 5. Press liquid through the syringe to wash out any particles from the tubing. 6. Attach the short tubing onto the outlet of the tip and press liquid through the syringe (Figure 5–23). Figure 5–23. Pressing liquid through the tip 7.
Maintenance Changing the tubing set Changing the tip band 1. Place the dispensing cassette onto the laboratory bench with the dispensing tips pointing down. 2. Unfasten the four cover retaining screws of the lower part of the dispensing cassette (Figure 5–24). Figure 5–24. Removing the cover plate of the lower part of the dispensing cassette 3. Remove the tip manifold, the tube fasteners and the rest of the tubing from the lower part of the dispensing cassette.
Maintenance Changing the tubing set Insert tube here Tip Figure 5–27. New tip band Fitting the new tubing set step by step 1. First place the new tubing set onto the laboratory bench with the tip band attached (Figure 5–28). Ensure that you save the eight calibration screws and the PEEK tubing weight from the previous tubing set. Tubing identification plate with tube lot number Figure 5–28. New tubing set with the tip band attached 2.
Maintenance Changing the tubing set Figure 5–30. Pressing the tube fasteners into place Figure 5–31. New tubing inserted into the lower part of the dispensing cassette 4. Attach the tension limiting wires into their slots (Figure 5–32). Slots for tension limiting wires Figure 5–32. Tension limiting wires attached 5. Fasten the four cover retaining screws of the lower part of the dispensing cassette (Figure 5–33). Figure 5–33. Replacing the lower part cover of the dispensing cassette 6.
Maintenance Changing the tubing set Figure 5–34. Parts belonging to the upper part of the dispensing cassette 7. Place the plastic sight window with the grooves pointing upwards onto the bottom cover of the upper part of the dispensing cassette, if it is not already there (Figure 5–35). Figure 5–35. Placing the sight window 8. Press the white tubing identification plate into place on the righthand side of the bottom cover of the upper part of the dispensing cassette (Figure 5–36). Figure 5–36.
Maintenance Changing the tubing set Figure 5–37. Pressing the tube fasteners into place 10. Fasten the calibration screws from the previous tubing into their places using a hexagonal screwdriver (Figure 5–38). Figure 5–38. Fastening the calibration screws 11. Insert the tension limiting wires into their slots into the upper part of the dispensing cassette (Figure 5–39). Slots for tension limiting wires Figure 5–39. Calibration screws fastened 12.
Maintenance Changing the tubing set Figure 5–40. Replacing the cover of the upper part of the dispensing cassette 13. Ensure that you do not get the tubing trapped or bent between the bottom and upper cover of the upper part of the dispensing cassette (Figure 5–41). Figure 5–41. Ensuring the tubing does not get trapped 14. Fasten the three cover retaining screws of the upper part of the dispensing cassette, leaving the front screw slightly open (Figure 5–42). Figure 5–42.
Maintenance Changing the tubing set Figure 5–43. Inserting the cover on the calibration screws 16. Fasten the front cover retaining screw (Figure 5–44). Figure 5–44. Lower and upper part of the dispensing cassette fitted 17. Turn the dispensing cassette so that the eight sight strips are visible (Figure 5–45). Look at the eight sight strips in the upper part of the dispensing cassette. You will notice that the tubes are not precalibrated. First open the calibration screw cover.
Maintenance Changing the tubing set Figure 5–46. Precalibrating the dispensing cassette 18. When the tube fasteners have all been adjusted roughly to the middle of the sight strip, close the calibration screw cover (Figure 5–47). Figure 5–47. Dispensing cassette precalibrated 19. Insert the eight tubes into the PEEK tubing weight either at this stage or earlier (Figure 5–48). The new tubing set has now been successfully fitted. Figure 5–48. Inserting the tubes into the PEEK tubing weight 20.
Maintenance Disposal of the dispensing cassette Disposal of the dispensing cassette Dispose of the dispensing cassette according to the legislation stipulated by the local authorities concerning take-back of the above-mentioned materials. The proposals for the procedures vary by country. Warning The dispensing cassette may contain biohazardous materials depending on the use of the cassette. It consists of: PEEK, silicone, stainless steel, PA (polyamide), and PP (polypropylene).
Chapter 6 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Accuracy verification (gravimetric) The verification and recalibration instructions cover the following three sections using the methods mentioned: accuracy verification (gravimetric); precision verification (photometric), and calibration (gravimetric). The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at 22°C ± 2°C.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Accuracy verification procedure ● Disposable gloves ● High-purity water (deionized distilled water) ● Photocopy of the accuracy verification worksheet from this manual (p. 83) 1. Install the dispensing cassette (see “Installing the cassette” on page 27). 2. Pull the rotor cover over the rotor. 3. Ensure that the priming vessel is installed. 4.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 10. Calculate the net weights of the dispensed strips (Net column), the dispensed volume per well in mg (Dispensed weight per well (mg) column), the dispensed volume per well in µl (Measured volume (µl) column), and the inaccuracy percentage (Inaccuracy % column) as in the example table. Refer to Table 6–8. If desired, you can also calculate the average dispensed volume and inaccuracy percentage for the whole plate.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Table 6–8. Example of an accuracy verification measured with a small tube cassette at 22°C Channel/ Strip Strip weight (g) Dispensed weight of one dispense per well (mg) Measured volume (µl) Inaccuracy % Tare Gross Net Strip Net x 1000 10 x 5 Weight per well x conversion factor (Z) (Measured volume – Set volume) x 100 / Set volume A 3.8707 4.3876 0.5169 10.3383 10.37 3.72 B 3.8699 4.3832 0.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Precision verification (photometric) This section describes the materials and steps needed for precision verification. Materials and equipment required for precision verification The following materials and equipment are required for precision verification: ● Microplate photometer set to measure absorbance at 540 nm (measurement range 0 – 2 Abs, resolution 0.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 4. Set the Multidrop Combi to deliver Ponceau S solution by inserting all eight channel tubes into the first Ponceau S stock solution vessel of the two. 5. Set the Multidrop Combi to dispense the first Ponceau S volume. Select the 96 standard (15mm) plate. 6. Press the PRIME button to predispense the tubes. 7. Insert the first empty, 96-well plate (Microplate A) into the plate carrier.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 14. Calculate the standard deviation results obtained for each microplate by using the following equation, or the STDEVP function in MS Excel or the σ function in a scientific calculator, and record the results in Table 6–11. Standard Deviation= √ (( ∑ (Abstest - Absmean )2 / 96 ) 15.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 2. Pull the rotor cover over the rotor. If the instrument is equipped with the SMART option and a SMART cassette is being used, the calibration data window appears and the calibration data can be viewed. Press the OK or STOP button to deactivate the calibration data window. 3. Ensure that the priming vessel is installed. 4.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 6. Open the calibration screw cover (Figure 6–49) on the upper part of the cassette. 7. Use high-purity water. Place the tube of channel A (Figure 11–54 on page 114) into the reservoir with water at room temperature (working temperature). 8. Press the PRIME button until the channel is dispensing continuously into the priming vessel. 9. Tare an analytical balance to 0 with one empty, dry 1 x 12 well microstrip.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Figure 6–50. Screwing a calibration screw according to the calibration results 14. Continue in the same way with each channel until all eight channels have been recalibrated and close the calibration screw cover. 15. Continue to set the calibration date. Select Calibration > Gravimetric > OK. Use the Left and Right arrow keys to select the day, month or year.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Calibration (standard tube volumetric) For volumetric calibration, use the following steps: 1. Insert the dispensing cassette into the working position so that the lower and upper parts of the dispensing cassette are inserted into their slots (Figure 4–14). 2. Install the tube of the calibration device into the tip of the channel to be recalibrated (Figure 6–51), for example, A. Figure 6–51.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Press OK. 7. Press the PRIME button until the water enters the calibration pipette. 8. Adjust the water level in the pipette to the lower mark (0.9) by pulling or pushing the syringe piston (Figure 6–52). Upper mark Lower mark Figure 6–52. Adjusting the liquid level first to the lower mark and then to the upper mark 9. Press the START button and wait until the water level stops.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Calibration screw cover opened Figure 6–53. Screwing the calibration screws according to the calibration results 11. Press the EMPTY and PRIME keys and the syringe to adjust the water level in the pipette to the lower mark and repeat steps 7 to 10 until the result is 1 ml. Three accurate calibrations must be obtained. Note The PRIME button must be pressed before START. ▲ 12.
Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Use the Left and Right arrow keys to select the day, month or year. Use the Up and Down arrow keys to enter the real-time calibration date. Press OK. 14. Close the calibration screw cover. Now the cassette contains the new calibration date, the Since last calibration shows 0%, but the Total usage remains unchanged and is always summed up.
Chapter 7 Technical Specifications General specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program. Table 7–12. General specifications General specifications Overall dimensions 355 mm (14 in.) (W) x 330 mm (12.9 in.) (D) x 220 mm (8.6 in.) (H) Weight (total) Instrument 9.1 kg (15.7 lbs.
Technical Specifications Safety specifications Cont. General specifications 10 µl: ± 5% (typical) > 10 µl: ± 5% (typical) Dispensing precision * 0.
Technical Specifications In conformity with the requirements Multidrop Combi conforms to the following requirements: 2006/95/EC (Low Voltage Directive) 2004/108/EC (Electromagnetic Compatibility Directive EMC) FCC Part 15, Subpart B/Class B (July 2004) 2002/96/EC (Waste of Electrical and Electronic Equipment) Safety performance: EN 61010-1:2001 (Ed.
Technical Specifications Remote control to Multidrop Combi Test standards Performance limits EN 55022:1998 + A1:2000 + A2:2003 Class B, 150 kHz – 1 GHz EN 61000-3-2:2000 Class A EN 61000-3-3:1995 + A1:2001 Remote control to Multidrop Combi ANSI C63.
Chapter 8 Frequently Asked Questions Q&As Q1: Can the dispensing cassettes of the Multidrop Combi be used in the Multidrop 384 and Thermo Scientific Multidrop DW? A1: Yes, the dispensing cassettes are interchangeable. The standard tube cassette (Cat. no. 24072670) can be used in the Multidrop 384 and Multidrop DW. The small tube cassettes (Cat. no. 2407390 and 2407395) can be used in the Multidrop Micro. Q2: Can the Dispensing cassette, machined covers (Cat. no.
Frequently Asked Questions Q&As Q8: What liquid is recommended to be used for washing the Multidrop Combi tubes? A8: It is recommended that you use deionized distilled water and/or detergent solutions, for example, 0.2 – 1% Tween followed by deionized distilled water. Q9: When do you have to calibrate the dispensing cassettes of the Multidrop Combi? A9: It is recommended that you recalibrate at least monthly, depending on the frequency of use and the liquids used.
Frequently Asked Questions Q&As Q15: Do you need specific plate adapters for the Multidrop Combi? A15: No, the Multidrop Combi has a fixed plate adapter and does not use any external plate adapters. Q16: What is the material of the Multidrop Combi priming vessel? A16: The Multidrop Combi priming vessel (Cat. no. N05640) is made of blue polypropylene (PP). Q17: Can the Multidrop Combi priming vessel be autoclaved? A17: Yes, it can be autoclaved.
Frequently Asked Questions Q&As 102 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Chapter 9 Troubleshooting Guide Note Do not use the instrument if it appears that it does not function properly. ▲ Error and warning codes When an error is detected, the current operation is terminated. After an error, it is best to abort the current run and restart from the beginning after the problem is fixed. The Multidrop Combi internal software has the following error messages (Table 9–13). Table 9–13. Error messages reported Code Error Cause Action 4 Pump position error.
Troubleshooting Guide Error and warning codes Press OK to acknowledge the error and then press PRIME. Press OK to acknowledge the error and then insert the priming vessel. Press OK to acknowledge the error and then pull the rotor cover over the rotor.
Troubleshooting Guide Hazards An example of a warning message that appears in the main view of the Multidrop Combi internal software is shown below. Press OK to acknowledge the warning and continue dispensing or press STOP to cancel and then reselect the dispensing volume. Hazards This instrument is designed to provide full user protection. When correctly installed, operated and maintained, the instrument will present no hazards to the user.
Troubleshooting Guide Service request protocol Environmental Infectious samples and corrosive fluids are commonly used with this equipment. The “hands-off” nature of the system allows the user to dispense into the reaction wells without getting into direct contact with these fluids. However, the wells that have been in contact with potentially hazardous fluids must be handled with utmost care. Always wear hand and eye protections as well as corrosive resistant laboratory coats.
Troubleshooting Guide Certificate of Decontamination Check that any necessary decontamination procedure has been carried out before packing. Refer to “Decontamination procedure” on page 66 and “Certificate of Decontamination” on page 107. Ensure that the Certificate of Decontamination (see Appendix B: “Certificate of Decontamination”) as well as the return authorization number (RGA) are sent with the instrument.
Troubleshooting Guide Certificate of Decontamination 108 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Chapter 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information. Multidrop Combi Dispensing cassettes Thermo Fisher Scientific Table 10–14. Instrument catalog number Code Instrument / System 5840300 Multidrop Combi, 100 − 240 V, 50/60 Hz 5840310 Multidrop Combi with SMART option, 100 − 240 V, 50/60 Hz Table 10–15.
Ordering Information List of accessories List of accessories Table 10–16. Codes for accessories Code Item N05616 Multidrop Combi User Manual N05640 Priming vessel, Multidrop Combi N05843 Priming vessel tube assembly and cap 1210550 Cord mains EURO 1210520 Cord mains UL/CSA 2305290 Serial cable F9/F25 N04001 USB A-B device cable * 030003 Tool 3mm DIN911 Allen key handled 95029370 Microtiter 1x12 strip assembled, UB, flat bottom (min.
Chapter 11 References Literature Editorial (2004): Multidrop bulk reagent dispenser family from Thermo Electron; precise dispensing in the microliter area allowing assay miniaturization. Innovations in Pharmaceutical Technology 4 (14), 36. Falconer M. et al. (2002): High-Throughput Screening for Ion Channel Modulators. Journal of Biomolecular Screening 7 (5), 460 – 465. Kiger A. A. et al. (2003): A functional genomic analysis of cell morphology using RNA interference. Journal of Biology 2, 27. Tanaka M.
References 112 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Appendix A 6 – 48-Well Plate Dispensing Instructions To dispense into a 6, 12, 24, or 48-well plate, leave some of the channels empty of liquid and select only some of the columns. 1. Insert the correct dispensing cassette and the priming vessel. Close the rotor cover. 2. Make sure you are in the Main menu. 3. Select 96 as the plate type and insert the 6, 12, 24, or 48-well plate. 4. Remove the tubes for the channels you leave empty from the tubing weight manually.
6 – 48-Well Plate Dispensing Instructions 8. Press the START button to start the protocol. The instrument dispenses the plate. 9. Press the EMPTY button to empty the dispensing tubing. 10. During prolonged standby, keep the cassette in its rest position. Remove the cassette when the maximum amount of plates are dispensed or you want to change the cassette type in use. Make sure you wash the cassette properly. 11. Power OFF the instrument. Figure 11–54.
6 – 48-Well Plate Dispensing Instructions 6-well plate settings First select a 96-well plate with an appropriate height. Figure A–55. Columns and channels for a 6-well plate setup Table A–18.
6 – 48-Well Plate Dispensing Instructions 12-well plate settings First select a 96-well plate with an appropriate height. Figure A–56. Columns and channels for a 12-well plate setup Table A–19.
6 – 48-Well Plate Dispensing Instructions 24-well plate settings First select a 96-well plate with an appropriate height. Figure A–57. Columns and channels for a 24-well plate setup Table A–20.
6 – 48-Well Plate Dispensing Instructions 48-well plate settings First select a 96-well plate with an appropriate height. Figure A–58. Columns and channels for a 48-well plate setup Table A–21.
Appendix B Certificate of Decontamination Name: Address: Tel./Fax: Name: Serial no.: A) I confirm that the returned items have not been contaminated by body fluids, toxic, carcinogenic or radioactive materials or any other hazardous materials. B) I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards.
Certificate of Decontamination 120 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Appendix C Thermo Scientific Multidrop Combi Feedback Form Instrument: Thermo Scientific Multidrop Combi Instrument serial no.: PURCHASED BY PURCHASED FROM Company/Institute Distributor Department Address Address Tel. Tel. Fax Date of delivery Internet home page Date of purchase Your application area Dr. Mr. Mrs. Ms.
Thermo Scientific Multidrop Combi Feedback Form 122 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Glossary antenna The tag antenna is the conductive element that enables the tag to send and receive data. The RF energy from the reader antenna is “harvested” by the antenna and used to power up the microchip, which then changes the electrical load on the antenna to reflect back its own signals. See RFID and RFID tag. EU European Union. backflush (empty) The operation of discharging the contents of the tubing of fluid. predispense The operation of filling a pump intake with fluid to expel the air.
Glossary: tension limiting wire tension limiting wires The tension limiting wires (Figure 4–10 and Figure 5–32) are designed to prevent the user from pulling the tubes too much as excessive tension may damage the tubes or change the calibration of the dispensing cassette. USB Universal serial bus.
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Glossary: 126 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific
Index 1 D 1536-well plates, 13, 14, 50, 62 dead volume, 99 decontamination, 63, 65, 66, 67, 68, 69, 107, 119, 123 deep-well plates, 110 default, 39, 40, 41, 44, 45, 46, 47, 48, 49, 55 protocols, 55 values, 39 deleting a protocol, 54, 103 dispensing, 36, 37, 38, 39, 40, 41, 42, 113, 114, 115, 116, 117, 118, 123 accuracy, 95, 96 direction, 35, 49 height, 35, 39, 47, 123 offset, 35, 39, 46 parameters, 37, 38, 82, 88 precision, 96 range, 26 speed, 15, 35, 40, 45, 86, 95, 96, 101 volume, 36, 37, 39, 40, 41,
F feedback form, 121 G glossary, 61, 123 guidelines for use, 6 H hazards, 4, 65, 105, 106, 119 home position, 14, 23, 32, 49 I icons, 34, 35 IEC, 123 info text, 33, 34, 61 installation, 3, 19, 20, 21, 23, 27, 68, 73, 82, 85, 87, 91, 121 intended use, 3, 13 internal software, 3, 32, 38, 101, 103, 105 K keyboard and display, 18, 32, 95 M maintenance, 3, 6, 65, 68, 69, 96, 107 markings, 123 N navigating, 32, 33, 34, 37 predispensing, 35, 48, 67, 69, 103, 123 volume, 35, 48 prime, 23, 32, 35, 36, 37, 48,
T technical specifications, 83, 95 tension limiting wires, 27, 28, 29, 73, 74, 76, 124 tip, 26, 27, 29, 30, 40, 70, 71, 72, 73, 91, 100, 109, 123 manifold, 72, 73 material, 26 transport, 19, 21, 22, 67, 68, 95, 106 lock, 21, 22, 68 package, 19, 67 troubleshooting, 23, 103, 107 tubing, 25, 26, 29, 30, 36, 50, 62, 70, 71, 72, 73, 74, 75, 76, 77, 79, 86, 99, 109, 113, 114, 115, 116, 117, 118, 123 material, 26 U USB, 14, 56, 95, 98, 110, 124 using the keyboard and display, 18, 32, 95, 105 V volume, 13, 15, 3
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