Version 1 February 2007 BioRobot® EZ1 DSP Workstation User Manual 9001360 9001360 1037181 1037181 QIAGEN QIAGEN GmbH, GmbH,D-40724 D-40724Hilden Hilden R1 Sample & Assay Technologies
Trademarks: QIAGEN®, BioRobot® (QIAGEN Group); Gigasept®, Lysetol®, Mikrozid® (Schülke & Mayr GmbH); DECON-QUAT® (Veltek Associates, Inc.). © 2007 QIAGEN, all rights reserved.
Contents Contents 1 Safety Information 1-1 1.1 Proper use 1-2 1.2 Electrical safety 1-2 1.3 Environment 1-4 1.4 Biological safety 1-5 1.5 Chemicals 1-6 1.6 Waste disposal 1-6 1.7 Mechanical hazards 1-7 1.8 Heat hazard 1-7 1.9 Symbols on the BioRobot EZ1 DSP 1-8 1.10 Additional symbols in this user manual 1-9 2 Introduction 2-1 2.1 About this user manual 2-1 2.2 2.2.1 2.2.2 2.2.
Contents 3.2 3.2.1 3.2.2 4 Internal features of the BioRobot EZ1 DSP Worktable Pipettor head Installation Procedures 3-5 3-6 3-9 4-1 4.1 Requirements 4-1 4.2 AC power connection 4-1 4.3 Hardware installation 4-3 5 General Operation 5-1 5.1 Overview 5-1 5.2 5.2.1 5.2.2 Inserting and removing the EZ1 Card Inserting the EZ1 Card Removing the EZ1 Card 5-2 5-2 5-3 5.3 5.3.1 5.3.
Contents 6.3 Weekly maintenance procedure 6-5 6.4 Reagents for decontamination 6-6 7 Troubleshooting 7-1 7.1 Detecting an error 7-1 7.
Contents This page intentionally left blank Contents-4 BioRobot EZ1 DSP Workstation User Manual 02/2007
Safety Information 1 Safety Information This manual contains information and warnings that must be followed by the user to ensure safe operation of the BioRobot EZ1 DSP workstation and to maintain the instrument in a safe condition. Possible hazards that could harm the user or result in damage to the instrument are clearly stated at the appropriate places throughout this manual. The following safety conventions are used throughout this manual.
Safety Information 1.1 WARNING Proper use Risk of personal injury and material damage [W1] Improper use of the BioRobot EZ1 DSP may cause personal injuries or damage to the instrument. The BioRobot EZ1 DSP must only be operated by qualified personnel who have been appropriately trained. Servicing of the BioRobot EZ1 DSP must only be performed by QIAGEN Instrument Service Specialists. Use only QIAGEN® components, otherwise your right to make a claim under the guarantee may be invalidated.
Safety Information WARNING Electrical hazard [W2] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live, and opening covers or removing parts is likely to expose live parts.
Safety Information Voltage rating label The following label appears on the back of the BioRobot EZ1 DSP. Ensure that the voltage rating specified on the label matches the voltage available at the installation site. 1.3 Environment Operating conditions WARNING Explosive atmosphere The BioRobot EZ1 DSP is not designed for use in an explosive atmosphere.
Safety Information 1.4 Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm). Samples Samples may contain infectious agents.
Safety Information 1.5 Chemicals WARNING Hazardous chemicals [W6] Some chemicals used with this instrument may be hazardous or may become hazardous after completion of the protocol run. Always wear safety glasses, gloves, and a lab coat. The responsible body (e.g.
Safety Information 1.7 Mechanical hazards The worktable of the BioRobot EZ1 DSP moves during operation of the instrument. WARNING Moving parts [W7] Never open the workstation door while the BioRobot EZ1 DSP is operating. WARNING Risk of overheating [W8] To ensure proper ventilation, maintain a minimum clearance of 15 cm and 30 cm at the rear and sides of the BioRobot EZ1 DSP, respectively. Slits and openings which ensure the ventilation of the BioRobot EZ1 DSP must not be covered. 1.
Safety Information 1.
Safety Information Symbol SN 1.
Safety Information Symbol on the Heating System Symbol near the Tip Rack 1-10 BioRobot EZ1 DSP Workstation User Manual 02/2007
Introduction 2 Introduction Thank you for choosing the QIAGEN BioRobot EZ1 DSP workstation. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument. 2.1 About this user manual This manual provides information about the BioRobot EZ1 DSP. It guides you systematically through the following sections: 1.
Introduction QIAGEN products. If you have any questions regarding the BioRobot EZ1 DSP or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques.
Introduction 2.3.1 Requirements for BioRobot EZ1 DSP users This table covers the general level of competence and training necessary for transportation, installation, use, maintenance, and servicing of the BioRobot EZ1 DSP.
Introduction This page intentionally left blank 2-4 BioRobot EZ1 DSP Workstation User Manual 02/2007
General Description 3 General Description The BioRobot EZ1 DSP workstation performs fully automated nucleic acid purification from up to 6 samples using magnetic particles. The automated steps include: Lysis of samples Binding of nucleic acids to magnetic particles Washing and elution of nucleic acids The user inserts an EZ1 Card containing protocol(s) into the BioRobot EZ1 DSP.
General Description 3.1 External features of the BioRobot EZ1 DSP Front View of the BioRobot EZ1 DSP Workstation 1 5 2 3 4 3.1.1 1 Workstation door 4 EZ1 Card 2 Control panel 5 Status LEDs 3 EZ1 Card slot Workstation door The workstation door can be manually opened by the user in order to gain access to the worktable. A protocol cannot be started until the workstation door is closed. 3.1.
General Description Keypad The following keys of the control panel are available to the user. Key Description 0–9 Selects a menu. START Initiates an action or starts a protocol. STOP Interrupts a protocol run. ESC Displays the previous menu or text. The other keys of the control panel are for service personnel only. Liquid-crystal display The LCD consists of 4 lines. There are 20 spaces per line. Liquid-Crystal Display 3.1.3 EZ1 Card slot The EZ1 Card slot accepts an EZ1 Card.
General Description could be lost, leading to a memory error. EZ1 Cards should not be exchanged while the workstation is switched on. Do not remove the EZ1 Card while the machine is switched on. Also, take care not to expose the EZ1 Card to electric shock, water, or dirt. EZ1 Card Completely Inserted into EZ1 Card Slot 3.1.
General Description 3.1.5 Connector panel The connector panel is located at the rear of the BioRobot EZ1 DSP. Connector Panel Power switch Power cord Fuse box Label indicating the correct fuses for the fuse box Connector for computer cable It contains the power switch, the socket for the power cord, the fuse box, and a connector for a computer cable (for service use only). 3.
General Description 3.2.1 Worktable The worktable contains various racks for holding the labware, samples, and reagent cartridges that are required for the protocol run. The worktable also contains a heating system for controlling the temperature of samples. The BioRobot EZ1 DSP Worktable Pipettor head Heating system Sample tubes Reagent cartridges in cartridge rack Sample tubes Tip holders with tips in tip rack Elution tubes Tip rack This rack is located in the front of the worktable.
General Description Tip Rack Both tip holders and filter-tips are made of polypropylene and available from QIAGEN. The filter-tips have a capacity of 50–1000 μl.
General Description Cartridge rack This rack is located behind the tip rack and holds up to 6 reagent cartridges. Cartridge Rack Reagent cartridges are made of polypropylene, contain prefilled reagents, and are available from QIAGEN. Reagent Cartridges Heating positions Reagent wells A reagent cartridge consists of 10 reagent wells and 2 heating positions. One heating position is a well, the other is a slot that can accept a tube.
General Description Heating system The heating system is located under the far end of the cartridge rack. It holds the heating positions of the reagent cartridges. Tray The tray is located under the racks and collects any drops of liquid that may fall. Tray 3.2.2 Pipettor head The pipettor head is mounted above the worktable and moves in the Z direction (i.e., up and down) in order to reach the samples and reagents on the worktable. The worktable itself moves in the Y direction (i.e.
General Description During operation of the BioRobot EZ1 DSP, the piercing unit first exposes the reagents in the reagent cartridges. The pipettor head then picks up filter-tips from the tip rack, and performs aspiration and dispensing operations at different locations on the worktable before ejecting the tips back into the tip rack.
Installation Procedures 4 Installation Procedures The BioRobot EZ1 DSP is a plug-and-play instrument, making unpacking and installation easy. A member of your group who is familiar with laboratory equipment should oversee the installation. 4.1 Requirements Site The BioRobot EZ1 DSP must be located out of direct sunlight, away from heat sources, and away from sources of vibration and electrical interference. Refer to Appendix A for the operating conditions (temperature and humidity).
Installation Procedures workstation. To preserve this protection feature, do not operate the workstation from an AC power outlet that has no ground (earth) connection. Installation of AC power cord Connect one end of the AC power cord to the socket located on the rear of the BioRobot EZ1 DSP, and the other end to the AC power outlet. The fuse box of the BioRobot EZ1 DSP is located under the socket for the power cord and contains 2 fuses which are labeled as follows: 110–120 V — a 6.
Installation Procedures WARNING 4.3 Electrical hazard [W12] Never install a fuse different from that specified in the user manual. Hardware installation The following items are delivered: BioRobot EZ1 DSP workstation Power cord (8 pieces) BioRobot EZ1 DSP accessories BioRobot EZ1 DSP Workstation User Manual 6GC teaching data sheet The BioRobot EZ1 DSP accessories include: Cartridge rack Tip rack Tray O-ring (pack of 6) and silicon grease Fuses (1 each: 6.3 A and 3.
Installation Procedures 2. Remove the outer top cardboard. Outer top cardboard Disposables and accessories Cardboard tray Corner shock absorbers Outer cardboard box Wrapped BioRobot EZ1 DSP Aluminum strap Corner shock absorbers Outer bottom cardboard 3. Take out the packages with the disposables and accessories. 4. Remove the cardboard tray. 5. Pull out the 4 corner shock absorbers at the top, and remove the outer cardboard box by pulling it up. 6.
Installation Procedures 7. Remove the inner top cardboard, and remove the BioRobot EZ1 DSP from the inner cardboard box. Inner top cardboard BioRobot EZ1 DSP Inner cardboard box 8. Remove all plastic sheets. BioRobot EZ1 DSP Plastic sheets Inner bottom cardboard Note: Be careful not to damage the surface of the BioRobot EZ1 DSP after removing the plastic sheets. Note: It is recommended to save the original packaging material for later transportation of the BioRobot EZ1 DSP.
Installation Procedures 1. Cut and remove the cable tie. Silica gel Cable tie Bubble wrap 2. Remove the bubble wrap and the silica gel. 3. Push the pipettor head upward. Pipettor head 4. Remove the bag of foam packaging material.
Installation Procedures Note: It is recommended to save the original packaging material for later transportation of the BioRobot EZ1 DSP. Installing the BioRobot EZ1 DSP 1. Adjust the fuse box if necessary (Section 4.2, page 4-1). 2. Connect the BioRobot EZ1 DSP to the power cord.
Installation Procedures This page intentionally left blank 4-8 BioRobot EZ1 DSP Workstation User Manual 02/2007
General Operation 5 General Operation This section describes how to operate the BioRobot EZ1 DSP. Before proceeding, it is recommended that you familiarize yourself with the features of the BioRobot EZ1 DSP by referring to Section 3. 5.1 Overview The steps for operating the BioRobot EZ1 DSP are presented below. Further details are provided later in this section. 1. Insert the EZ1 Card completely into the EZ1 Card slot. 2. Switch on the BioRobot EZ1 DSP. 3.
General Operation 5.2 CAUTION 5.2.1 Inserting and removing the EZ1 Card Damage to the instrument [C3] Ensure that the BioRobot EZ1 DSP is switched off before you insert or remove the EZ1 Card. Otherwise, the card and/or the instrument may be damaged. Inserting the EZ1 Card 1. Lift up the cover of the EZ1 Card slot. 2. Insert the EZ1 Card into the EZ1 Card slot. Orientate the card so that the picture faces to the left and the triangle symbol is at the bottom.
General Operation Ensure that the card is completely inserted into the slot. It is extremely important that the EZ1 Card is completely inserted to avoid memory error and loss of essential instrument data. 3. Close the cover of the EZ1 Card slot. 4. Switch on the BioRobot EZ1 DSP (Section 5.3.1, page 5-4). Note: Do not remove the EZ1 Card while the machine is switched on. 5.2.2 Removing the EZ1 Card 1. Switch off the BioRobot EZ1 DSP (Section 5.3.2, page 5-5). 2. Lift up the cover of the EZ1 Card slot.
General Operation 3. Press the button at the bottom of the EZ1 Card slot. The EZ1 Card is ejected. 5.3 Switching the BioRobot EZ1 DSP on and off 5.3.1 Switching on the BioRobot EZ1 DSP 1. Insert the EZ1 Card (Section 5.2.1, page 5-2). 2. Switch on the BioRobot EZ1 DSP at the rear power switch. 3. The LCD displays the following text for a few seconds. This text indicates that the BioRobot EZ1 DSP is initializing. All motors move to their home positions.
General Operation 4. After initialization, the main menu appears. You can now operate the BioRobot EZ1 DSP. 5.3.2 Switching off the BioRobot EZ1 DSP 1. If you had run a protocol, refer to “End of the protocol run” on page 5-7. 2. Switch off the BioRobot EZ1 DSP at the rear power switch. 5.4 WARNING Opening and closing the workstation door Moving parts [W7] Never open the workstation door while the BioRobot EZ1 DSP is operating.
General Operation 5.4.1 Opening the workstation door 1. Slide your fingers under the door and push it upward. 2. Rest the door on the door lock that is located near the top of the BioRobot EZ1 DSP. 5.4.2 Closing the workstation door 1. Press the door down to disengage the door lock. 2. Gradually lower the door until it rests on the worktable. Take care that your fingers do not get caught between the door and worktable. 5.5 WARNING 5.5.
General Operation 2. Start worktable setup by pressing the appropriate number key (e.g., “1” or “2”). For details, refer to the handbook supplied with the EZ1 DSP Kit you are using. 3. Follow the instructions displayed by the LCD. 4. Open the workstation door and set up the worktable according to the messages displayed by the LCD (Section 5.6, page 5-8). 5. Close the workstation door. The protocol run cannot start until the door is closed. 6. Press “START” to start the protocol run.
General Operation 1. Press “STOP”. The protocol run pauses and the LCD displays the following text. 2. To continue the protocol run, press “START”. The protocol run continues from where it had stopped. 3. To cancel the protocol run, press “STOP”. The LCD displays the main menu. Then follow these steps: Ensure that the workstation door is closed Press “1” to display “Manual” in the LCD 5.
General Operation 5.6.1 Removing and replacing the tray The tray is located under the worktable and can be removed for cleaning: 1. Switch off the BioRobot EZ1 DSP. 2. Push the worktable toward the back of the BioRobot EZ1 DSP. 3. Remove the tray using its handle. Reverse this procedure to replace the tray. To avoid instrument damage, ensure that the tray is positioned correctly. 5.6.2 Loading the reagent cartridges Note: Do not remove the foil from the reagent cartridges. 1.
General Operation 4. Slide the reagent cartridges into the cartridge rack in the direction of the arrow, as shown below, until you feel resistance. Press down the cartridges until they click into place. 5. Return the cartridge rack to the worktable. Ensure that the 2 heating positions of each cartridge fit into the heating system.
General Operation 5.6.3 Loading the elution tubes, filter-tips, and sample tubes 1. Remove the tip rack from the worktable. 2. Load the elution tubes into row 1 of the tip rack. Label the elution tubes before you load them into the rack. Ensure that the caps of the tubes are removed before you start the protocol. 3. Place the filter-tips into the tip holders. Ensure that the tips are positioned correctly in the holders. 4. Load the tip holders into rows 2 and 3 of the tip rack.
General Operation 6. Return the tip rack to the worktable. Ensure that the tips, tip holders, and tip rack are correctly positioned on the worktable.
Preventive Maintenance 6 Preventive Maintenance The following preventive maintenance procedures must be carried out to ensure reliable operation of the BioRobot EZ1 DSP: Regular preventive maintenance — after each protocol run Daily preventive maintenance — after the last protocol run of the day Weekly preventive maintenance — every week Annual preventive maintenance — every year; removal and replacement of parts are carried out by QIAGEN Instrument Service Specialists only WARNING Risk of electr
Preventive Maintenance 6.1 Regular maintenance procedure WARNING Risk of personal injury and material damage [W1] Improper use of the BioRobot EZ1 DSP may cause personal injuries or damage to the instrument. The BioRobot EZ1 DSP must only be operated by qualified personnel who have been appropriately trained. Servicing of the BioRobot EZ1 DSP must only be performed by QIAGEN Instrument Service Specialists. WARNING Hazardous chemicals and infectious agents [W11] The waste contains samples and reagents.
Preventive Maintenance 3. Press “3” to select “Clean pierce unit”. The piercing unit moves downward. 4. Wipe the piercing unit using a soft tissue moistened with 70% ethanol. The piercing unit is sharp. Double gloves are recommended. Piercing unit 5. Wipe the piercing unit using a soft tissue moistened with distilled water.
Preventive Maintenance 6. Press “ESC” to return the piercing unit to its original position. 7. Clean the tray and racks with 70% ethanol and then with distilled water. 8. Clean the BioRobot EZ1 DSP worktable with 70% ethanol and then with distilled water. 9. Wipe the other surfaces of the workatable with a diluted neutral soap solution and then with distilled water. You can now run another protocol or switch off the BioRobot EZ1 DSP. 6.
Preventive Maintenance 6. Wipe the O-rings of the tip adapters with tissue. 6.3 Weekly maintenance procedure Perform the daily maintenance procedure before you perform the weekly maintenance procedure. To maintain good contact between tip adapters and filter-tips and to prevent liquid leaking from the tips, grease the O-rings of the tip adapters every week: 1. Apply a small amount of silicon grease to the end of a filter-tip. 2. Apply the silicon grease to the surface of the O-rings. 3.
Preventive Maintenance 6.4 Reagents for decontamination The following disinfectants and detergents are compatible with surfaces and components of the BioRobot EZ1 DSP system. Use according to manufacturers’ instructions for effective disinfection. WARNING Toxic fumes [W13] Do not use bleach to clean or disinfect the instrument. Bleach in contact with salts from the buffers can produce toxic fumes. Mikrozid® Liquid (Schülke & Mayr GmbH; www.schuelke-mayr.
Troubleshooting 7 Troubleshooting 7.1 Detecting an error If a protocol run is interrupted due to an error: The red LED flashes An alarm sounds The LCD displays an error message LCD Displaying an Error Message The second line of the error message gives the error code (see list below). The third line indicates the line number of the protocol at which the error occurred. Record the error code and the line number, and contact QIAGEN Technical Services. Then reset the BioRobot EZ1 DSP: 1.
Troubleshooting 7.
Glossary 8 Glossary Term Description Cartridge rack A metal rack that accommodates reagent cartridges on the worktable. Connector panel The panel on the rear of the BioRobot EZ1 DSP. It contains the power switch, the socket for the power cord, the fuse box, and a connector for a computer cable. Control panel The user interface that allows the user to operate the BioRobot EZ1 DSP. The control panel consists of an LCD and a keypad. Elution tube A polypropylene, screw-capped 1.
Glossary Term Description O-ring A ring that is fitted at the bottom of a tip adapter. It is required for good contact between the tip adapter and a filter-tip. Pipettor head The component of the BioRobot EZ1 DSP that aspirates and dispenses liquid and separates magnetic particles. The pipettor head moves up and down above the worktable and contains 6 syringe pumps, each of which is connected to a tip adapter.
Glossary Term Description Workstation door The main door at the front of the BioRobot EZ1 DSP. When open, it provides complete access to the worktable. Worktable The surface of the BioRobot EZ1 DSP that contains racks and is where samples, reagent cartridges, and disposable labware are loaded. The worktable moves backwards and forwards to present different samples and reagents under the pipettor head.
Glossary This page intentionally left blank 8-4 BioRobot EZ1 DSP Workstation User Manual 02/2007
Appendix A Appendix A Technical data Environmental conditions Power 100–120/200–240 V AC ± 10%, 50/60 Hz, 300 VA Fuses 6.3 A (250 V) (for 110–120 V AC) 3.15 A (250 V) (for 220–240 V AC) WARNING Electrical hazard [W12] Never install a fuse different from that specified in the user manual.
Appendix A Mechanical data and hardware features Dimensions Width: 0.32 m (13 in.) Height: 0.54 m (21 in.) with door closed 0.73 m (29 in.) with door open Depth: 0.50 m (20 in.) Mass 25.8 kg (57 lb.
Appendix A Pipettor head Contains 6 high-precision syringe pumps, each containing a tip adapter which attaches to filter-tips. Each tip aspirates and dispenses 50–1000 μl liquid. Pipetting accuracy is as follows: 50–100 μl ± 5% 100–1000 μl: ± 2% Syringe pumps are air-filled. Liquids containing salts, alcohol, solvents, and/or magnetic particles can be aspirated and dispensed. Air gaps can be aspirated to prevent aspirated liquid from dripping.
Appendix A Labware Reagents are loaded onto the worktable using the reagent cartridges. These cartridges are already prefilled with reagents by QIAGEN. Several reagent cartridges are accommodated together on the worktable by a cartridge rack. Samples are loaded onto the worktable using 2 ml sample tubes. Steps which require heating occur on the heating system, which accommodates the heating positions of the reagent cartridges. Purified nucleic acids are collected in 1.5 ml elution tubes.
Appendix A QIAGEN accepts its responsibility in accordance with the specific WEEE recycling requirements and, where a replacement product is being supplied by QIAGEN, provides free recycling of its WEEE-marked electronic equipment in Europe. If a replacement product is not being purchased from QIAGEN, recycling can be provided upon request at additional cost. To recycle electronic equipment, contact your local QIAGEN sales office for the required return form.
Appendix A This page intentionally left blank A-6 BioRobot EZ1 DSP Workstation User Manual 02/2007
Appendix B Appendix B Warranty statement Thank you for your purchase of QIAGEN instrumentation. Your instrument has been carefully tested to ensure optimum operating efficiency and reproducibility of results. QIAGEN warrants that all new instrumentation manufactured by QIAGEN will correspond to the product specifications and be free from defects in workmanship and materials for a period of twelve (12) months from the original date of shipment.
Appendix B Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.
Index Index C Cartridge rack, 3-8 Connector panel, 3-5 Control panel, 3-1, 3-2 E Elution tubes, 3-6 loading, 5-11 EZ1 Card, 3-1, 3-2 inserting, 5-2 removing, 5-3 slot, 3-2, 3-3 F Filter-tips, 3-6 loading, 5-11 Fuse box, 3-5, 4-2 H Heating positions, 3-8 Heating system, 3-1, 3-9 L LCD, 3-2 M Magnet, 3-9 Magnetic particles, 3-1 Maintenance, 6-1 daily, 6-4 decontamination, 6-6 weekly, 6-5 O Operating conditions, 1-4 P Piercing unit, 3-10 Pipettor head, 3-1, 3-9 Power cord, 3-5 Power requirements, 4-1 P
Index Tip rack, 3-6 Tips, 3-6 loading, 5-11 Tray, 3-9 removing, 5-9 Troubleshooting, 7-1 Index-2 W Waste disposal, 1-6, A-4 Workstation door, 3-1, 3-2 closing, 5-6 opening, 5-6 Worktable, 3-1, 3-5 setting up, 5-8 BioRobot EZ1 DSP Workstation User Manual 02/2007
QIAGEN Distributors and Importers Please see the back cover for contact information for your local QIAGEN office. Argentina Tecnolab S.A. Tel: (011) 4555 0010 Fax: (011) 4553 3331 E-mail: info@tecnolab.com.ar Estonia Quantum Eesti AS Tel: +372 7301321 Fax: +372 7304310 E-mail: quantum@quantum.ee Malaysia RESEARCH BIOLABS SDN. BHD. Tel: (603)-8070 3101 Fax: (603)-8070 5101 E-mail: biolabs@tm.net.my Bosnia-Herzegovina MEDILINE d.o.o. Tel.
www.qiagen.
