Quick start installation guide D-0184-1, Rev 1.
Welcome Here’s everything you need to install your device and to check the system is ready for use. Pre-installation Before you install the device the following should be available: MinIT Just follow the steps on the following pages in order to: Power Supply • Setup the MinIT™ for use Wireless-Compatible Tablet, Laptop or SmartPhone • Power on the device 1 x Oxford Nanopore MinION™ • Run the Hardware check to confirm the device has shipped successfully MinION USB 3.
About your MinIT Usage advice Technical Specification: Recommendations: Component Specification Size W 65 x L 120 X D 40 mm Weight 290 g Compute Specification Processing: GPU accelerators (ARM Processor 6 Cores, 256 Core GPU), 8 GB RAM Connections 1 x USB 3.0 1 x USB 2.0 1 x 1 GB Ethernet (for Networking) On-Board Software Linux OS and MinIT OS (including MinKNOW) Place the MinIT on a stable and clean laboratory bench.
Your MinIT What’s in the box: 1 x MinIT Device 1 x Power supply 4 x Country-Specific Adapters
MinIT overview Useful information about MinIT setup: • GUI is defined as a Graphical User Interface • For Windows OS, please refer to A4 to A6 later in this document • For Mac OS, please refer to B4 to B6 later in this document White LED display indicates the device is powered Blue LED display ‘flash’ indicates the Wi-Fi is being enabled Green LED display ‘flash’ indicates the Wi-Fi has connected successfully Red LED display ‘flash’ indicates the Wi-Fi connection has not been successful.
Setting up your MinIT 1 Unpack the device Place the MinIT on a clean surface. 2 Attach the required cables 1 x MinION via USB 2.0 port (lower of two, power port right) 1 x (Optional) USB SSD Storage 1 x (Optional) Ethernet • Ethernet • USB 2.0 (for MinION) • USB 3.0 (for SSD storage) • Power socket 3 Power Supply Connect the power supply to the AC mains power and the MinIT. The MinIT logo will illuminate white when powered.
Connecting your MinIT – Windows OS A4 Identify the MinIT ID The ID for the MinIT is shown above in the red box, on the underside label. Note this number (e.g. MT-110087) is required for Wi-Fi connection. A5 Connect to the MinIT (Wi-Fi) Using a Wi-Fi compatible device, connect to the Wi-Fi with the ID of your MinIT (A4). Login with the Security Key: WarmButterflyWings98 A6 Opening the MinKNOW GUI Open network sharing and go to \\mt1XXXXX (MinIT ID (A4)). This is your MinIT’s location on the network.
Connecting your MinIT – Mac OS B4 Identify the MinIT ID The ID for the MinIT is shown adjacent in the red box, on the underside label. Note this number (e.g. MT-110087) is required for Wi-Fi connection. B5 Connect to the MinIT (Wi-Fi) Using a Wi-Fi compatible device, connect to the Wi-Fi with the ID of your MinIT (B4). Login with the Password: WarmButterflyWings98 B6 Opening the MinKNOW GUI Under Shared, click on MT-1XXXXX (MinIT ID (B4)). This is your MinIT’s location on the network.
Running the Hardware Check 7 Plug MinION Into MinIT Plug the MinION into the USB 2.0 port and insert your CTC flow cell into the MinION. 8 Select CTC The CTC will appear numerically in the GUI. Select the ‘flow cell’ icon by ticking the box below it. 9 Click the “Check hardware” button This can be found at the bottom of the screen, shown in the red box above. USB 2.
Confirm the CTC is loaded correctly A display will appear asking you to check the CTC is inserted correctly. Once confirmed click “Start test”. 11 Check the messages The message tab on the right will show “Calibration finished successfully”, followed by “Hardware check has completed successfully”. 12 Your hardware is working correctly Once the configuration run has completed, the Hardware is ready for a sequencing experiment. Please refer to the Nanopore Community for additional protocols.
Powering off the device 13 Close the GUI Ensure the MinION is not running. Close the GUI through the “x” in the top right corner. 14 Power off device Remove MinION from MinIT and switch power off at the power source. The power cable can then be removed from the MinIT.
Additional information Cleaning the MinIT Safety Information Should any leaks develop over the course of loading a flow cell, or during an experiment, please absorb excess liquid using a laboratory tissue and clean the area using an IPA wipe. The MinIT is for Research Use Only. Please dispose of the cleaning materials into a biohazard waste bin. Please ensure you have reviewed the MinIT safety information before you start using the device.
