Instructions for use SLIDEVIEW VS200 Optical Microscope and Accessory
Any copyrights relating to this manual shall belong to Olympus Soft Imaging Solutions GmbH. We at Olympus Soft Imaging Solutions GmbH have tried to make the information contained in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose.
Contents 7 1 About this manual 1.1 1.2 1.3 1.4 7 7 8 8 Notes on qualifications Intended use Other applicable documents Symbols in this documentation 2 Safety 2.1 2.2 2.3 2.4 2.5 2.6 9 9 9 9 10 13 13 Categories and symbols in the safety instructions Caution labels Caution labels on the product General safety instructions Safety instructions for the laser Safety instructions for X-Cite Turbo and X-Cite Xylis 3 Hinweise zum Aufstellen 14 3.
9.4.3 Removing transportation lock from SCARA robot arm’s counterweight 10 Mounting of components 37 37 37 38 39 10.1 Scanner 10.1.1 Objectives 10.1.2 Immersion Objectives 10.1.3 Phase Contrast (PH) objectives 11 Mounting fluorescence components 40 11.1 IX3-RFACA and IX3-RFALFE 11.2 Fluorescence filter wheels or camera adapter for monochrome camera 11.2.1 U-FFWR (Motorized fast reflected light filter wheel) 11.2.2 Add or replace filter (U-FFWR) 11.2.
16 PC operating system language 17 VS200 ASW software setup 18 VS200 device configuration 77 78 85 86 86 87 88 89 90 90 91 92 94 95 18.1 Activate the motorized polarizer 18.2 Activate the VS200 liquid dispenser 18.3 Device settings - objectives 18.4 Device settings - filter 18.5 Manual device configuration 18.5.1 ORCA monochrome camera 18.6 Device customization 18.6.1 ORCA camera adjustments 18.6.2 Setup phase contrast (PH) observation method 18.6.3 Setup polarization (Pol) observation method 18.6.
21.4 Lens Correction 21.5 Shading correction BFMono 21.6 Magnification Test Scan 21.7 Shading correction for fluorescence observation methods 21.8 Shading correction for darkfield (DFMono) 21.9 Shading correction for PhaseContrast (PH) 21.10 Channel-XY-Shift 22 Cleaning the system 22.1 22.2 22.3 22.4 22.
1 About this manual 1 About this manual This manual for the VS200 system is for Olympus technicians as well as users of the VS200 system who have been trained by Olympus. The manual contains important information on how to operate the VS200 system safely and correctly. In addition, this manual describes how to unbox and install the system as well as how to install further components. These tasks must expressly only be performed by Olympus.
1 About this manual 1.3 Other applicable documents Familiarize yourself with all of the other manuals for the components of the system. Take special note of the safety instructions they contain. 1.
2 Safety 2 Safety If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product may also be damaged. Always use the product as outlined in this instruction manual. 2.
2 Safety Position of caution labels U-FFWO Loader Im Bereich des Mikroskoptisches Nosepiece Flap door Flap door motor 2.4 General safety instructions Do not plug anything into or unplug anything from the ports when the system is connected to the power supply. The system is not intended to be used for general microscopy tasks. No guarantee is provided on an image file.
2 Safety 5. Radiation prevent from magnetic or electromagnetic sources in close proximity. Prevent from radioactive contamination 6. Chemicals/ toxic substances prevent from substances that are corrosive, potentially infectious, toxic or otherwise hazardous to health Regarding Monitor 1. Do not use this product near water. 2. Do not place this product on an unstable cart, stand or table. If the product falls, it could be seriously damaged. 3.
2 Safety Shading / unneven light intensity For specimens with very sparse samples with a lot of empty background and rather small fluorescence emittance(e.g. FISH samples), it can occur that images acquired with Fluorescence illumination show a variance in background illumination ("shading"). If the product is being used in a network environment, note the following points. Make sure that the network has appropriate virus protection.
2 Safety 2.5 Safety instructions for the laser 1. The loader uses a laser sensor. Never remove the warning and caution labels on the product. The semiconductor laser for loader incorporated in this product it designated as a product of the following class. CLASS 1 LASER PRODUCT(IEC60825-1:2007 / IEC608251:2014) This product complies with 21 CFR 1040. 10 and 1040. 11 except for deviations pursuant to Laser Notice No. 56, dated June 24, 2007. 2. Never attempt to remove the cover using a tool.
3 Hinweise zum Aufstellen 3 Hinweise zum Aufstellen A fully equipped VS200 system weighs approx. 190 kg. Consider this weight when selecting the work surface. Beachten Sie die Angaben zum Gewicht, wenn Sie das VS200 System bzw. die Komponenten anheben. See Specifications auf Seite 24. Die Aufstellfläche muss für eine Tragfähigkeit von mindestens 190 kg ausgelegt sein. Place the VS200 system on a sturdy level table or bench, taking care not to block the ventilation slots of the scanner and loader.
3 Hinweise zum Aufstellen VS200 System with loader Without fluorescence With fluorescence Height 885 mm 885 mm Area of work surface (table) 1800 mm x 800 mm 1800 mm x 800 mm Storage conditions The maximum permissible range for the ambient temperature for storage of the VS200 system is -25 to 55°C. The maximum permissible relative humidity range for storage is 10% to 95%. The maximum permissible temperature change rate for storage is 30°C/h.
4 Tools and accessories 4 Tools and accessories 2,5 mm, 3 mm with ball end, 4 mm, 5 mm hex keys OLYMPUS VS200 System - 16 - Instructions for use
5 Scope of supply 5 Scope of supply VS200 Single Tray and VS200 Multi Tray Loader 1st pallet VS200 base unit 2nd pallet Housing panel left Housing panel right (different variant for single tray and multi tray loader) Housing panel back Housing back panel cover Housing panel top front Housing panel top back Housing beam splitter cover 4 handles to carry the system PLN2x UPLANXAPO20x 1 1x3 inch tray VS200 standard computer with mouse and keyboard HP TFT 27 inch Instruction manual Calibration slide v2.
5 Scope of supply Fluorescence kit A Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube TV1XC U-FFWR X-Cite Xylis Fluorescence kit B Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube 2x Black-out filter 1x Glas filter U-FFWO T3 U-FFWR X-Cite Xylis Fluorescence kit C The Fluorescence kit C is not available in all countries.
5 Scope of supply Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube TV1XC X-Cite Turbo OLYMPUS VS200 System - 19 - Instructions for use
6 System diagram 6 System diagram VS200 system diagram 2 Fluorescence Cameras Loader Options 3 8 7 VS20-304M FL camera VS304M 1 VS20-BU-L VS200 Slide Scanner Base Unit for Loader VS20-LOADER VS200 Slide Loader Unit HAMAMATSU C1344020CU Hamamatsu Orca Flash 4.
6 System diagram Component A TV1XC X U-FFWR X U-FFWO T3 Xcite Xylis (IR), whitelight LED B C X X X X D X X Xcite Turbo, switchable LED X X D Optional - Filter sets Component A B C Recommended filter set single-band exciter multi-band emitter single-band exciter single-band emitter single-band multi-band emitexciter ter single-band emitter Monochrome cameras Component A B C D VS304M 0 0 0 0 Orca Flash 4.
7 Trays 7 Trays 7.1 Tray description 7.2 (1) Button to open/close the spring for inserting a slide (2) Slide pocket (3) Slide position (4) Indicator for label area (5) Indicator for tray insertion VS200 Tray types The VS200 systems can be equipped with four different tray types for different slide formats. All trays can be either used with the VS200 ST (Single Tray Load) system or VS200 MTL (Multi Tray Loader) system.
7 Trays Additional tray types 3 slides in 2x3 inch (76 x 52 mm) 1 slide in 3x4 inch (76,3 x 103 mm) 1 slide in 4x5 inch (76,3 x 128 mm) OLYMPUS VS200 System Add image - 23 - Instructions for use
8 Specifications 8 Specifications VS200 Single Tray Load (STL) Intended specimen Optical frame VS200 Multi Tray Loader (MTL) Observable specimen Glass slide with cover glass Size of glass slide Standard tray: width: 25 mm – 26.5 mm, length: 75 mm – 76.5 mm, thickness: 0.9 mm - 1.2 mm Optional trays: 1) Width: 51 mm – 53 mm, length: 75 mm – 76.5 mm, thickness: 0.9 mm - 1.2 mm 2) Width: 100 mm – 102 mm, length: 75 mm – 76.5 mm, thickness: 0.9 mm - 1.
8 Specifications VS200 Single Tray Load (STL) Scan VS200 Multi Tray Loader (MTL) Image correction Shading correction, auto white balance, true color Capacity 6 slides maximum (VS200 single tray) 210 slides maximum (VS200 multi tray loader) Pixel resolution (color cam- 20X (NA 0.80): 0.274 µm/pixel Options: era) 10X (NA 0.40): 0.548 µm/pixel 40X (NA 0.95): 0.137 µm/pixel 40XO (NA 1.40): 0.137 µm/pixel 60XO (NA 1.42): 0.091µm/pixel 100XO (NA 1.45): 0.055µm/pixel Environment Scan time Approx.
9 Unboxing 9 Unboxing The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 9.1 VS200 Scanner The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 9.1.1 Mounting of handles - VS200 scanner The units described below must be assembled and adjusted by Olympus.
9 Unboxing This chapter describes how to remove the transportation locks from the VS200 scanner after shipping. ATTENTION The transportation locks must be remounted each time before the unit is transported. Make absolutely sure not to lose the transportation locks and the mounting material.
9 Unboxing View from above View from below 9.2.2 Removing transportation lock from transmitted light condenser (BX3-UCD8A) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. ATTENTION The transportation locks must be remounted each time before the unit is transported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (2,5 mm, 3 mm) 1.
9 Unboxing 2. Remove the transportation lock from the transmitted light condenser. To do so, remove the 2 hex screws (size 3) indicated in the figure. 3. Pull the transportation lock forwards and down to remove it.
9 Unboxing 9.3 VS200 Loader The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. CAUTION Risk of injury and risk of device damage if components are dropped The loader or other components might drop when you are moving them. Injury to your feet or damage to the device can result. Make sure that all handles for transportation are screwed in completely and tight.
9 Unboxing 8. Make sure that all handles for transportation are screwed in completely and tight. 9. Remove all blue tape stripes for the grounding flags as well as the tape that secures the front door. 10. Remove the tape at the bottom left corner and take out the bubble foil pocket which contains the cable for the loader connection. 11. Open the door and remove the six desiccant bags.
9 Unboxing 12. Use two persons to lift the loader onto a very stable table to the right side of the VS200 scanner. The center of gravity is at the right backside of the system. 13. Remove the handles and keep them as they might be needed in the future. ATTENTION The system should not be repositioned by the end user. If the system has to be repositioned an Olympus sales representative has to be contacted first. 9.3.1 VS200 Trays 1. Unbox the VS200 trays carefully and handle them with care.
9 Unboxing Overview - transportation locks ATTENTION Mögliche Geräteschäden durch Inbetriebnahme mit montierten Transportsicherungen! Wird der Loader in Betrieb genommen, ohne dass zuvor die Transportsicherungen vollständig demontiert wurden, kann es zu Geräteschäden kommen. Demontieren Sie vor Inbetriebnahme des Loaders unbedingt alle Transportsicherungen! ATTENTION The transportation locks must be remounted each time before the unit is transported.
9 Unboxing Transportation lock (1) ATTENTION The transportation locks must be remounted each time before the unit is transported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3 mm) 1. Remove the red transportation lock from the VS200 loader’s tray hotel. To do so, loosen the 4 hex screws (size 3 mm) indicated in the figure. 9.4.
9 Unboxing Transportation lock (2) and (3) ATTENTION The transportation locks must be remounted each time before the unit is transported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3 mm) 1. Remove the red two-part transportation locks from the SCARA robot arm. To do so, loosen the 8 hex screws (size 3 mm) indicated in the figure. 9.4.
9 Unboxing Transportation lock (4) ATTENTION The transportation locks must be remounted each time before the unit is transported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3 mm) 1. Remove the red transportation lock from the brass colored counterweight of the SCARA robot arm. To do this, loosen the 3 hex screws (size 3 mm) indicated in the figure. Make sure that the screws to do not fall inside the housing.
10 Mounting of components 10 Mounting of components 10.1 Scanner 10.1.1 Objectives The VS200 system is shipped with no objective mounted in the IX3-nosepiece. 1. Make sure that the power of the VS200 system is switched off! 2. Open the door of the VS200 scanner. 3. Make sure that the objective is clean and dust free before you mount it. 4. Depending on your VS200 kit you need to mount different objectives. The objectives should be mounted into fixed positions as per the list below.
10 Mounting of components Position list for objectives The position of the nosepiece is indicated at the center of the IX3nosepiece. Nosepiece position Objective 1 2x PLN 2 20x UPLXAPO 3 10x UPLXAPO 4 40x UPLXAPO 5 Any immersion objective or phase contrast objective 6 4x UPLFLN If you mount objectives in a different order you must subsequently adjust all device settings and observation methods.
10 Mounting of components ATTENTION It is not possible to change the type of immersion medium once the glass laboratory bottle has been filled with either oil or silicone immersion medium. Otherwise damage can occur to the liquid dispenser. Before the VS200 system can be shipped (e.g. for repair) all liquids have to be removed from the system. Please contact the Olympus Customer Support for detailed information.
11 Mounting fluorescence components 11 Mounting fluorescence components The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Skip this chapter if you have a brightfield system only. In the box with the fluorescence components (A, B, C or D) you find all parts needed to setup fluorescence for the VS200 system. Carefully unbox all components and follow the instructions. 11.
11 Mounting fluorescence components 2. Use a 3mm hex key to remove the two hex socket screws which hold the slider of the field stop. 3. Make sure that the field stop is pushed in completely. 4. Goto the scanner and open the front door. 5. Remove the six 2.5 mm hex socket screws which hold the black tamper protection plate like shown in the image below. 6. Remove the two 3 mm hex socket screws from the left and the right securing brackets from the VS200 system as shown in the image below.
11 Mounting fluorescence components 7. Fold up the two securing brackets. 8. Remove the two 4 mm hex socket screws from the RFAA supporting bracket, which is attached to the frame at the back of the system. 9. Insert the IX3-RFACA/ IX3-RFALFE unit into the system. Make sure that it fits into the rails on the left and the right side and flip down the brackets.
11 Mounting fluorescence components 10. Tighten the two 3 mm hex socket screws to fix the brackets. 11. Attach the RFALFE supporting bracket again at the back of the system's frame. Insert the two 4 mm hex socket screws but don't tighten them.
11 Mounting fluorescence components 12. Push the bracket upwards until it attaches to the IX3-RFALFE and tighten the two hex socket screws. 13. Make sure the power is switched off and attach the IX3-RFACA cable to the socket at the back of the IX3-RFACA. 14. Attach the black tamper protection plate again and tighten the six hex socket screws.
11 Mounting fluorescence components Finally the system should look like on the image below. 11.2 Fluorescence filter wheels or camera adapter for monochrome camera The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Depending on the customer’s configuration you either have to mount only one or two fast filter wheels. The following chapter describes how to mount the different filter wheels. 11.2.
11 Mounting fluorescence components IMPORTANT Before you mount the U-FFWR make sure you insert all excitation filters into the filter wheel. Note the position of the individual filters as you might need them later for the observation method adjustment. If you mount them as set out in the table below, less adjustments will have to be made later.
11 Mounting fluorescence components 2. Use the short CAN bus cable (35cm) to connect the U-FFWR to the VS200 system. Note the plug orientation indicated by the red dot. 3. In case you do not have a U-FFWO connect the terminator to the second plug at the U-FFWR. 11.2.2 Add or replace filter (U-FFWR) 1. Make sure that the power of the VS200 system is switched off. 2. Remove the backside top panel. See Top panel auf Seite 66. 3.
11 Mounting fluorescence components 4. Insert the filter into an empty position. Make sure that you orient the filter correctly when you insert it. Refer to the U-FFWR instruction manual for more information. The correct orientation for Semrock emission filters is with the arrow towards the camera. The correct orientation for Chroma emission filters is with the arrow pointing away from the camera. 5. Write down the position number of the filter as you will need it later. 6.
11 Mounting fluorescence components 10. Configure the filter. See Device settings - filter auf Seite 88. 11.2.3 U-FFWO T3 (Motorized fast observation filter wheel) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Hex key (2.0) to lock the tubus Hex key (3.0) to open the filterwheel Small spanner to adjust the height of the tubus Spanner to fix the tubus 1.
11 Mounting fluorescence components Before you mount the U-FFWO T3, make sure you insert all emission filters into the filter wheel. Note the position of the individual filters as you might need them later for the observation method adjustment. Refer to chapter Add or replace filter (U-FFWO) auf Seite 51 for filter orientation. U-FFWO T3 position 25 mm emission filter 1 DAPI 432/36 2 FITC 515/30 3 CY3 595/31 4 CY5 680/42 5 CY7 6 Black-out filter 7 Black-out filter 8 25 mm Glass 2.
11 Mounting fluorescence components 4. Connect the CAN-bus cable (which you find in the inside of the scanner) to the lower connector and the CAN-terminator to the upper connector. 11.2.4 Add or replace filter (U-FFWO) Hex key (3 mm) 1. Make sure that the power of the VS200 system is switched off. 2. To add or replace a 25 mm emission filter in the U-FFWO, open the cover of the filter wheel using a 3 mm hex key. 3. Insert the filter into an empty position.
11 Mounting fluorescence components Refer to the U-FFWO instruction manual to chapter [Inserting the Optical Filters] for more information. The correct orientation for Semrock emission filters is with the arrow towards the camera. The correct orientation for Chroma emission filters is with the arrow pointing away from the camera. 4. Write down the position number of the filter as you will need it later. 5. Configure the filter in the VS200 ASW software. To do this, open the [Device Settings] dialog box.
11 Mounting fluorescence components Depending on the fluorescence kit, VS200 offers three different monochrome cameras: VS304M (iDS) Orca Flash 4.0 (V3) ORCA Fusion VS200 When you switch on an Orca camera wait at least one minute before you start the VS200 ASW software otherwise the camera will not be recognized. If you have a TV1.0x adapter on top of the beam splitter, screw the camera on top of the adapter. See Orientation of the monochrome camera auf Seite 53 for the orientation of the camera. 11.3.
11 Mounting fluorescence components The orientation of the ORCA Fusion VS200 camera should be with the water cooling connectors facing the front side of the system. If your fluorescence kit contains a U-FFWO T3, follow the instructions below to mount a monochrome camera. For more details refer to the U-FFWO manuals. This example describes how to attach an ORCA Flash 4.0 camera to the U-FFWO T3. The attachment of VS304M (iDS) and ORCA Fusion VS200 is identical.
11 Mounting fluorescence components (1) camera (2) C-mount adapter and counternut 3. Fasten the C-mount adapter (not the counternut) clockwise to the camera by using the large wrench tool as indicated below. (1) fasten clockwise (2) large wrench, small side 4. Hand-tighten the camera and C-mount adapter to the U-FFWO. Be aware to tighten the camera orientation lock first to prevent slippage of the locking mechansism. To do so, tighten the rotation locking screw using a 2 mm hex key.
11 Mounting fluorescence components (1) camera (2) tighten rotation locking screw (3) U-FFWO 5. As a final step, adjust orientation and parfocality of the camera, and then lock the camera via counternut to prevent accidential de-adjustment. How to adjust the camera adapter (tubus) is described in chapter Camera adapter auf Seite 139. 11.4 X-Cite adapter The units described below must be assembled and adjusted by Olympus.
11 Mounting fluorescence components 1. Depending on the system configuration attach the X-Cite adapter to either the IX3-RFALFE flange (A) directly, or - if a U-FFWR is present - attach it to the flange of the U-FFWR (B). Option A: IX3-RFALFE flange Option B: U-FFWR 11.5 4x Objective For proper system performance when acquiring fluorescence overview images the UPFLN4X has to be mounted on position 6 of the IX3-nosepiece. See Mounting of components auf Seite 37 for installation instructions. 11.
11 Mounting fluorescence components Follow the safety precautions at all times during operation and maintenance of this product. Non-observance may result in eye injury or damage to the system. CAUTION Risk of injury to the eyes Do not look at operating lamp/LED as it can emit UV light. Never look into the light emitting end of the light guide. The light could severely damage the eye if the light is observed directly.
11 Mounting fluorescence components 1. Open the door of the VS200 scanner. 2. Slide the door of the IX3-RFACA to the left to insert an IX3 filter cube. 3. Insert a new filter cube into the correct position (position 2) as shown in the image. The correct position is printed inside of the IX3-RFACA and is hard to read as the IX3-RFACA is mounted upside down. If you cannot read it make sure that the filter is placed between the position index 7 and 8 as shown in the image below. 4.
11 Mounting fluorescence components 8. Select the [Acquire] > [Devices] > [Device Settings] command to open the [Device Settings] dialog box. 9. Configure the filter cube. See Device settings - filter auf Seite 88. 11.7.3 U-FDICT filter cube The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Mount an optional U-FDICT for polarized light acquisition.
11 Mounting fluorescence components 11.7.4 X-Cite Turbo The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The excitation filters in the X-Cite Turbo have to be placed as indicated in the boxes in the picture below (the arrows show the filter orientation). Refer to the X-Cite Turbo manual how to mount the filters.
12 Assembly of the housing for scanner 12 Assembly of the housing for scanner Before starting with the assembly of the housing make sure the main power is disconnected! The VS200 system is shipped with only the front door mounted. Side, back and top panels have to be attached on site. 1. Remove the four handles. Follow the correct order for mounting the elements of the housing so that you don't run into any trouble. 12.1 Left panel The units described below must be assembled and adjusted by Olympus.
12 Assembly of the housing for scanner The left side panel has two black quick lock pins (1) at the top and two rubber ring sockets (2) near the bottom. Additionally it has two connectors (3) for the grounding flags which need to be connected with the cables coming from the VS200 frame. On the left side of the VS200 scanner you find two quick locks sockets (1) at the top and two small pins (2) close to the bottom as well as two grounding flag connectors with cables attached (3). 1.
12 Assembly of the housing for scanner into the sockets. 12.2 Right Panel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Depending on your system configuration (with or without loader) the VS200 system comes with two different right side panels. The side panel of the loader variant has an opening for loading the trays. The mounting of the right side panel is similar to the left panel.
12 Assembly of the housing for scanner the sockets. 12.3 Back panel The back panel needs to be attached before mounting the top panels. It is fixed with four knurled head screws. The three knurled head screws at the top are part of the top panel and are tightened later. 1. Guide the three cables from the backside of the VS200 system through the back panel like shown in the image below.
12 Assembly of the housing for scanner 2. Guide the optional liquid light guide through the hole in the back like shown in the image below. 3. Place the back panel against the backside of the system and tighten the four knurled hex screws. 12.4 Top panel Top panel - first part The top panel consists of two parts. You have to start with the back part (large part) of the top panel. The panel has two quick lock pins (1) in the front and two guiding holes in the back (2).
12 Assembly of the housing for scanner 3. Gently push the top panel to the front of the system (where the front door is mounted). If the first part of the top panel is correctly mounted it should look like this: Mount the beam splitter cover In case you have a brightfield system only you have to mount the beam splitter cover now. top view bottom view 1. Put the round cover on top of the beam splitter flange and fix it by tightening the 3mm headless hex screw.
12 Assembly of the housing for scanner On the VS200 scanner you find two quick lock sockets(1) and two quick lock pins (2). On the panel itself you find the corresponding parts; two quick locks sockets (1) and two quick lock pins (2). 1. To make the assembly easier connect the two grounding flags already to the grounding plugs (3) of the top panel. 2. Open the front door and put the short top panel parallel on the scanner. 3.
12 Assembly of the housing for scanner 4. In case you use a U-FFWO make sure that the cable is guided through the housing like in the image below. If the top panels are mounted correctly, it should look like this: 5. Connect the two grounding flags to their counter plugs on the right and left side like shown in the image below.
13 Cabling 13 Cabling The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The VS200 PC has a label on its backside indicating where to plug in the individual cables. It is important to connect all cables according to the connection scheme as otherwise components might not work correctly. Please refer to the following table: Cable Type Location PC position VS-364M (s/w) USB 3.
13 Cabling USB 2.0 Hub USB 2.0 Back of VS200 I X-Cite Turbo USB 2.0 Back of xCite Turbo D X-Cite Turbo Power cord Back of xCite Turbo Power socket Xylis USB 2.0 Back of Xylis D Xylis Power cord Back of Xylis U-FFWR CAN Bus VS200 internal U-FFWO CAN Bus VS200 internal HP LCD screen Display port Bottom of LCD screen HP LCD screen Power cord Bottom of LCD screen HP mouse USB 2.0 E HP keyboard USB 2.
14 Assembly of the loader housing 14 Assembly of the loader housing The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The VS200 loader is shipped with the two side panels already mounted. Follow the order of panel mounting as described in the chapter below. 14.1 Top panel The units described below must be assembled and adjusted by Olympus.
14 Assembly of the loader housing 2. Subsequently tighten all other knurled screws. 14.3 Front panels The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1. Mount the horizontal and vertical front panel as described in the following steps. Before After 2.
14 Assembly of the loader housing 3. Connect the grounding flag to the vertical panel and push the panel towards the system so that the quick lock sockets of the panel fit onto the quick lock pins of the loader.
15 Verbindung VS200 Scanner und VS200 Loader 15 Verbindung VS200 Scanner und VS200 Loader 15.1 Mechanical Connection VS200 scanner and VS200 loader The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes the mechanical connection between the VS200 scanner and the VS200 loader. Hex screwdriver (size 5 mm) 1.
15 Verbindung VS200 Scanner und VS200 Loader 6. Push the VS200 loader onto the connector on the VS200 scanner. 7. Tighten the 2 hex screws (size 5 mm) which attach the connector of the VS200 scanner to the VS200 loader. 8. Remove the remaining handles from the VS200 scanner and the VS200 loader. 9. Reconnect the VS200 system to the power supply.
16 PC operating system language 16 PC operating system language If you want to change the language of the operating system follow the instructions below. Japanese and Chinese are pre-installed on the VS200 PC. 1. Open the Windows Settings and select [Time & Language]. 2. Click on the language you would like to use.
17 VS200 ASW software setup 17 VS200 ASW software setup The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes the installation of VS200 ASW on a VS200 system PC. 1. Turn on the PC. 2. To start the setup process, go to D:\OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE\SetupMain\ and run the Setup.exe. 3. Click [Yes] to allow changes to the PC device. 4.
17 VS200 ASW software setup 5. To provide the licenses open the envelope of the license cards and enter the license keys. You might have more than one license key depending on the configuration of your kit. Available license keys are: VS200 ASW 3.1 VS-FLUO VS-CNV 6. Accept the license agreement. To activate the licences at customer site the PC needs internet access. If no internet access is available the licences can also be activated offline.
17 VS200 ASW software setup 7. Change language (English is default). Proceed with [Next]. 8. Change installation location (not recommended). Proceed with [Next]. 9. A change of the temporary file location (D:\Temp) is not recommended. Proceed with [Next].
17 VS200 ASW software setup 10. If your kit includes a different monochrome camera than the IDS-VS-304M select either ORCA-Flash4.0 or ORCA-Fusion VS200 and proceed with [Next]. 11. Select [Add icon to the desktop] and [Add manuals shortcut to the desktop] and proceed with [Next]. 12. Start setup by clicking [Next].
17 VS200 ASW software setup 13. Finish the setup process by clicking the [Finish] button. 14. In the following dialog box, click the [Close] button and do not install any example data or tools. 15. Turn on the VS200 system.
17 VS200 ASW software setup 16. To start the software double-click the VS200 ASW icon. 17. At the first start agree to the End-User License Agreement and proceed with [Continue]. The following splash screen is displayed while the VS200 system is initializing. The initialization process might need up to two minutes. Error messages If you see the error message below once the software is up and running please refer to Troubleshooting auf Seite 179.
17 VS200 ASW software setup In most cases this message occurs because you are using a wrong device configuration. Refer to chapter VS200 device configuration auf Seite 85 to change or adapt the VS200 device configuration. If you see the following error message it means that you are using a VS200 loader which is not yet configured in the device list. Refer to chapter VS200 device configuration auf Seite 85 to add the loader to the system configuration.
18 VS200 device configuration 18 VS200 device configuration 1. On the start page of the software click the [Device Settings] button. The software will automatically switch to the [Manual control] layout and open the [Device Settings] dialog box. 2. In the [Device Settings] dialog box click on the [Device List] button to open the [Device List] dialog box. 3. VS200 ASW offers five predefined device configurations to choose from.
18 VS200 device configuration 4. If you are using the VS200 loader select the [Microscope] tab and select in the [Sample loading] list the [VS200 MTL Slide Loader] entry. 18.1 Activate the motorized polarizer All VS200 SLIDEVIEW systems are equipped with a polarization angle changer. By default the functionality of the motorized polarizer is switched off. 1. Use the [Acquire] > [Devices] > [Device List] command to open the [Device List] dialog box. 2.
18 VS200 device configuration 18.3 Device settings - objectives A VS200 SLIDEVIEW kit is supplied with a 2x and a 20x objective ex-works. However, the predefined device configuration already contains a 2x, 4x, 10x, 20x and 40x objective. You need to delete the objectives that are not installed from the nosepiece settings in the [Device Settings] dialog box. Open the [Device Settings] dialog box 1. Use the [Previously Used Layout] button to go to a different layout.
18 VS200 device configuration Refer to chapter Setup phase contrast (PH) observation method auf Seite 92 to adjust the [Device Customization] dialog box for phase contrast acquisition. 3. In case you want to install and configure an immersion objective please also check that the [Auto-Escape] function is checked. 4. In the [Device List] dialog box make sure that the [VS200 Liquid Dispenser] check box is selected. See Activate the VS200 liquid dispenser auf Seite 86. 18.
18 VS200 device configuration 3. Type in the name of the filter in the [Filter] field. Example of filter configurations for U-FFWR and U-FFWO in the [Device Settings] dialog box. 4. Add additional filters if required by selecting the appropriate position and selecting the name of the filter. 5. Click the [OK] button to save the changes. 6. Subsequently you have to configure an observation method in the [Device Customization] dialog box to be able to use the new filter.
18 VS200 device configuration 18.5.1 ORCA monochrome camera If your VS200 kit contains an ORCA Flash 4.0 or ORCA Fusion VS200 camera you need to manually add it to the device list as those two cameras are not part of the default setup. 1. Open the [Device List] dialog box and go to the [Camera] tab. 2. In the [Camera 2] list select either the ORCA Flash 4.0 or ORCA Fusion VS200. 3. Make sure that the correct camera adapter magnification (1.0x) is selected in the [Device Settings] dialog box.
18 VS200 device configuration 18.6.1 ORCA camera adjustments 1. Use the [Acquire] > [Devices] > [Device Settings] command to open the [Device Settings] dialog box. 2. Select the [BFMono] observation method (OM). 3. Unlock the [BFMono] observation method by clicking on the lock icon. 4. In the [Status] picklist, set the Orca camera to the status [Use]. 5. In the [Image type] picklist, change the [Current] entry to the [16-bit grayscale] entry. 6.
18 VS200 device configuration 7. Set the correct VS200 LED lamp voltages for the individual objectives. Refer to chapter VS200 LED lamp voltages auf Seite 97 for detailed information. 18.6.2 Setup phase contrast (PH) observation method If you want to add a phase contrast observation method do the following: 1. Select the [Acquire] > [Devices] > [Device Customization] command to open the [Device Customization] dialog box. 2. Select the [BFMono] observation method. 3.
18 VS200 device configuration 7. Select the e.g. 20x phase contrast objective and select the [Use with this objective] check box. Refer to the following table for the correct assignment of the different phase contrast inserts to the correct magnification: Objective magnification BX3 condenser PH insert 10x U-PH1-S 20x U-PH1-S 40x U-PH2-S 60x U-PH3-S 100x U-PH3-S 8. Select the [BX3 Top Lens] entry from the [Available components] and select the [Top Lens] > [In] option for the 20x PH objective.
18 VS200 device configuration 18.6.3 Setup polarization (Pol) observation method If you want to add a polarization observation method do the following: 1. Select the [Acquire] > [Devices] > [Device Customization] command to open the [Device Customization] dialog box. 2. In the [Device Customization] dialog box select the [BF] observation method. 3. Use the [Copy Observation Method] button observation method. to make a copy of the 4. Type in a name, e.g. Polarization. 5.
18 VS200 device configuration 8. Select the [Polarization Angle Changer] entry from the available components and choose the [Adjust] entry in the [Status] pick list. 9. Select the [BX3 Polarizer] and set the Status to [Use]. 10. Use the slide control to rotate the motorized polarizer or enter a defined angle value in the field. 18.6.4 Create or adjust an observation method The easiest way to create a new observation method (OM) e.g.
18 VS200 device configuration Configuring an observation method 1. Select an observation method (e.g. CY3) in the [Name] list. This list contains all of the observation methods that have already been defined. 2. Click the [Copy Observation Method] button to create a copy of the observation method. 3. Enter a name for the observation method in the [Enter a New Observation Method Name] dialog box. 4. In the [Available Components] list select the [Fluorochromes] entry. 5.
18 VS200 device configuration shading correction. See Shading correction for fluorescence observation methods auf Seite 160. 18.7 VS200 LED lamp voltages 18.7.1 Orca Flash 4.0 voltages (%) Observation method Objective BFMono DFMono PHMono (AS 75%) (AS max) (AS 75%) 2x PLN 0.6 not defined not defined 4x UPLFLN 0.6 - not defined 10x UPlanXApo 0.8 - - 20x UPlanXApo 0.5 not defined - 40x UPlanXApo 0.
18 VS200 device configuration 18.7.3 VS-304M voltages (%) Observation method Objective BFMono DFMono PHMono (AS 75%) (AS max) (AS 75%) 2x PLN 1.6 ND ND 4x UPLFLN 2.0 100 ND 10x UPlanXApo 1.7 100 - 20x UPlanXApo 1.7 not defined 100 40x UPlanXApo 4.
19 How to insert a slide into a tray 19 How to insert a slide into a tray 1. To insert a slide into a tray push the button on the tray away from the slide pocket to open. 2. Hold the button in place and put a slide into the slide pocket with the label facing the top as indicated on the tray [Label side]. 3. Push the slide to left side and release the button. 19.
19 How to insert a slide into a tray 3. Click the [Close] button to close the flap door and the exchange dialog. 19.2 Insert a tray into the VS200 loader 1. To insert a tray into the VS200 loader use the [Exchange Trays] function of the VS200 ASW software to drive the loader into the loading/unloading position. Click the [Exchange Trays] button on the start page of the VS200 ASW. 2.
20 Calibrate VS200 using the Olympus Calibration Slide 20 Calibrate VS200 using the Olympus Calibration Slide This chapter describes how to calibrate a VS200 SLIDEVIEW System. 3 mm hex key with ball end The VS200 ASW software offers a dedicated calibration wizard for all necessary calibrations. The image shows the [Calibrations] dialog box. In order to get optimal results, we recommend using the VS Olympus Calibration Slide v2.0 to calibrate the system. Layout of the Calibration Slide (Version 2.
20 Calibrate VS200 using the Olympus Calibration Slide 1. Insert the VS-calibration slide into position 3 of the slide tray. 2. Click the [Exchange Trays] button on the start page of the VS200 ASW software to insert the tray. 3. Click the [Select slide for calibration] button. 4. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. The software switches to the [Manual control] layout. 5.
20 Calibrate VS200 using the Olympus Calibration Slide 20.1 Stage limits - Z Axis The X and Y axes do not have to be calibrated again. However, the Z axis calibration is dependent on the glass slide thickness. Therefore it is necessary to calibrate the Z axis. 1. In the [Calibrations] dialog box select the [Stage Limits] entry and click the [Calibrate] button. 2. Proceed with [Next] and follow the instructions of the wizard.
20 Calibrate VS200 using the Olympus Calibration Slide 3. Select the [Z Axis] check box and continue with [Next]. 4. At the top right in the menu bar click the [Manual control] entry to switch the layout. 5. In the [Manual Control] layout, change to the 20x objective. 6. In the [Camera Control] tool window click the [Live] button to switch to live mode. 7. Use the stage navigator to move to the center of the slide. 8. Use the [Ctrl] + mouse wheel combination to focus the sample.
20 Calibrate VS200 using the Olympus Calibration Slide 10. Click the [Finish] button to finalize the calibration process.
