20 Calibrate VS200 using the Olympus Calibration Slide 20.2 Camera adapter calibration for iDS (VS-264C) camera 3 mm hex key with angle Standard Olympus 3 mm hex key The iDS color camera is mounted and calibrated ex-works. The parfocality might change due to temperature changes. Therefore do not check this calibration with a “cold” system (e.g. directly after assembling the system). Wait at least 30 min with the system switched on to heat it up.
0 Calibrate VS200 using the Olympus Calibration Slide 2. The system will use the objective with the highest magnification and switch to live mode. If the image is not in focus click the [Autofocus] button to perform an autofocus.
20 Calibrate VS200 using the Olympus Calibration Slide 3. Select an area on the calibration slide were it is possible to focus with both the 40x and 2x objectives like in the screenshot below. 4. Click the [Next] button to continue. If the focus distance between the objectives differs too much, the wizard tells you to either shorten or stretch the camera adapter. 5.
20 Calibrate VS200 using the Olympus Calibration Slide 9. Click the [Finish] button to finalize the calibration process.
20 Calibrate VS200 using the Olympus Calibration Slide 20.3 Camera-To-Stage Rotation The standard for the camera-to-stage rotation is +/- 0.1°. However the closer you get to zero the better. 1. Select the [Camera-To-Stage Rotation] entry. 2. Click the [Calibrate] button to start the wizard. 3. Use the stage navigator to move to the center of the calibration slide as shown in the image below. 4. Select the 10x or 20x objective from the menu bar.
20 Calibrate VS200 using the Olympus Calibration Slide 5. If the value is beyond the acceptable rotation angle, loosen the headless hex screw used to fix the TV 0.63 adapter at the flange of the beam splitter. 6. After a slight rotation, tighten the screw again. If the value is negative, tighten the screw counter clockwise. If the value is positive, tighten the screw clockwise. Click the [Back] button in the window. Then click the [Autofocus] button in the next dialog box to perform the test again. 7.
20 Calibrate VS200 using the Olympus Calibration Slide 20.4 XY Objective Shift / Parfocality To carry out a proper XY shift/parfocality calibration use the position of the calibration slide shown in the screenshot below. 1. In the [Calibrations] dialog box select the [XY Objective Shift / Parfocality] entry. 2. Click the [Calibrate] button to start the wizard.
20 Calibrate VS200 using the Olympus Calibration Slide 3. Select all objectives. Immersion objectives are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 4. Make sure that the functions [Calibrate XY objective centering] as well as [Calibrate Z parfocality] is selected. 5. Proceed with [Next]. 6. If the image seems not to be in focus perform an autofocus.
20 Calibrate VS200 using the Olympus Calibration Slide 7. The wizard will automatically select all of the objectives that are present and calculate the correction factors. If the Z parfocality for the 40x, 20x and 10x objectives is below 4µm everything is fine. The Z parfocality distance for the 2x objective should not exceed 20µm. If the values do not match the standard please check whether all objective are screwed in completely and whether the calibration slide is clean. Repeat the calibration. 8.
20 Calibrate VS200 using the Olympus Calibration Slide 20.5 Shading Correction (Brightfield) Use the stage navigator to move to the ‘empty’ area of the calibration slide. The slide must be very clean (free of dust particles which will disrupt the shading correction procedure). 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button.
20 Calibrate VS200 using the Olympus Calibration Slide 2. Select the [BF] entry as the observation method and proceed with [Next].
20 Calibrate VS200 using the Olympus Calibration Slide 3. When you carry out the calibration for the first time do not skip the acquisition of the dark current correction image. For all future calibrations you can skip the acquisition of the dark current correction image.
20 Calibrate VS200 using the Olympus Calibration Slide 4. Proceed with [Next].
20 Calibrate VS200 using the Olympus Calibration Slide 5. After the dark current correction image calibration, select all objectives. Immersion objectives are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 6. Make sure that the options for [Shading correction] and [White balance] are checked.
20 Calibrate VS200 using the Olympus Calibration Slide 7. Proceed with [Next]. The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. Subsequently go back to the position where the calibration slide is inserted.
20 Calibrate VS200 using the Olympus Calibration Slide 8. Click the [Next] button to start the image acquisition process for the 2x objective. After the acquisition is complete, the calibration process automatically moves to the next objective. The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-calibration slide. 9. Proceed with all other objectives in the same way.
20 Calibrate VS200 using the Olympus Calibration Slide 10. Click the [Finish] button to finalize the calibration process. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38.
20 Calibrate VS200 using the Olympus Calibration Slide 20.6 Shading correction of the label area 20.6.1 Preparation of calibration slide For the shading correction calibration of the label scan you need to prepare a proper slide. If a label printer is used, take an empty sticker and stick it on a normal glass slide as shown in the image below. If the customer does not use a label printer take a piece of white copy paper and glue it onto the slide. Example of a slide with an empty sticker 1.
20 Calibrate VS200 using the Olympus Calibration Slide 2. Insert the tray by clicking the [Exchange Trays] button on the start page of the VS200 ASW software. If you use the loader put the tray in e.g. position 2. 3. Click the [Lock Door] button in the VS200 ASW software to lock the door. 4. Click the [Select Slide for Calibration] button on the start page of the VS200 ASW software. 5. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. 6.
Calibrate VS200 using the Olympus Calibration Slide Start the calibration 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button. 2. Select the [Label Scan] entry for the observation method and proceed with [Next].
20 Calibrate VS200 using the Olympus Calibration Slide 3. When you carry out the calibration for the first time do not skip the acquisition of the dark current correction image. For all future calibrations you can skip the acquisition of the dark current correction image. Proceed with [Next].
20 Calibrate VS200 using the Olympus Calibration Slide 4. Proceed with [Next] to start the acquisition process 5. Proceed with [Next].
20 Calibrate VS200 using the Olympus Calibration Slide 6. After the dark current image calibration select all non-immersion objectives. Immersion objectives shall be calibrated separately. Proceed with [Next]. IMPORTANT Actually, the label shading correction must only be performed for the 2x objective. However you should do it for all other objectives as well as otherwise there will always be an exclamation mark next to the entry. 7. Use the stage navigator to move e.g.
20 Calibrate VS200 using the Olympus Calibration Slide 9. Focus on the empty label sticker and click the [Next] button to proceed. Use the focus up and down buttons like shown in the image below. After the acquisition is done the calibration process automatically moves to the next objective. 10. Proceed with all other objectives in the same way.
20 Calibrate VS200 using the Olympus Calibration Slide 11. Click the [Finish] button to finalize the calibration process.
20 Calibrate VS200 using the Olympus Calibration Slide 20.6.2 Shading correction for polarization (Pol) The result of the shading correction for polarization is dependent on the value for the [Polarization Angle Changer] which is set in the Polarization observation method. See Setup polarization (Pol) observation method auf Seite 94. If you change the angle you subsequently have to redo the shading correction. 1.
20 Calibrate VS200 using the Olympus Calibration Slide 3. Skip the acquisition of the dark current correction image. 4. Select all objectives.
20 Calibrate VS200 using the Olympus Calibration Slide 5. Make sure that the options for [Shading correction] and [White balance] are checked. The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. Subsequently go back to the position where the calibration slide is inserted. 6. Click the [Next] button to start the image acquisition process for the 2x objective.
20 Calibrate VS200 using the Olympus Calibration Slide After the acquisition is complete, the calibration process automatically moves to the next objective. The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-calibration slide. 7. Focus on the part of the slide that contains the sample and subsequently move to a very clean area to acquire the shading image. 8.
20 Calibrate VS200 using the Olympus Calibration Slide ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38.
20 Calibrate VS200 using the Olympus Calibration Slide 20.7 Magnification Test Scan 1. In the [Calibrations] dialog box select the [Magnification Test Scan] entry and click the [Calibrate] button. 2. Now you can select whether you want to perform the magnification test scan for all objectives or only for certain ones. Immersion medium objectives are calibrated in the same wizard.
20 Calibrate VS200 using the Olympus Calibration Slide 3. Use the stage navigator to move to center (cross hair) of slide and autofocus. 4. Proceed with [Next]. 5. Click the [Finish] button to finalize the calibration process. In case the calibration value is not within the standard go back to the [Camera-To-Stage Rotation] calibration and redo all calibrations.
21 Additional calibrations for a fluorescence system 21 Additional calibrations for a fluorescence system 1. Insert the VS-calibration slide into position 3 of the slide tray. 2. Click the [Exchange Trays] button on the start page of the VS200 ASW software to insert the tray. 3. Click the [Select slide for calibration] button. 4. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. The software switches to the [Manual control] layout. 5.
21 Additional calibrations for a fluorescence system mand. 21.1 Camera adapter The parfocality might change due to temperature changes. Therefore do not perform this calibration with a “cold” system (e.g. directly after assembling the system). Wait at least 30 min with the system switched on to heat it up. If you are using objectives with a cover slip correction collar, it is recommended to adjust it prior to all subsequent calibrations. The limit for the focus distance is +/- 20µm.
21 Additional calibrations for a fluorescence system 2. Use [Ctrl] + mouse wheel to fine focus.
21 Additional calibrations for a fluorescence system 3. Use the stage navigator to move to a similar position on the calibration slides to that shown in the image below. 4. Perform an autofocus. 5. If the focus differs too much you need to manually adjust the U-FFWO T3 adapter. Adjust the camera adapter 1. Release the camera rotation locking screw. Hold the camera with one hand to prevent camera rotation. Use the small wrench to adjust parfocality of the camera until the image on the screen is in focus.
21 Additional calibrations for a fluorescence system (1) Release locking screw. (2) Keep hold of camera to prevent rotation. Adjust parfocality. (3) 2. Lock the parfocal setting by tightening the counternut. To do so, pull the large wrench clockwise to fasten the counternut while holding the small wrench steady. (1) Hold steady. (2) Fasten clockwise.
21 Additional calibrations for a fluorescence system 3. Adjust the camera rotation by hand and tighten the camera rotation locking screw. (1) Rotate camera. (2) Tighten locking screw. 4. Execute an autofocus again and check the final result. 5. Repeat until the rotation value meets the standard. 6. Finalize the process by clicking the [Finish] button.
21 Additional calibrations for a fluorescence system 21.2 Camera-To-Stage Rotation The standard for the camera-to-stage rotation is +/- 0.1°. However the closer you get to zero the better. 1. In the [Calibrations] dialog box select the [Camera-To-Stage Rotation] entry and click the [Calibrate] button. 2. Use the stage navigator to move to the center of the calibration slide.
21 Additional calibrations for a fluorescence system 3. Select the 10x objective from the menu bar and perform an autofocus. 4. If the value is beyond the acceptable limits, loosen the screws in the beam splitter flange to rotate the U-FFWO together with the camera. 5. After a slight rotation, tighten the screws again and click the [Back] button in the dialog box. 6. Then click the [Autofocus] button in the next dialog box to perform the test again. 7. Repeat until the value meets the standard. 8.
21 Additional calibrations for a fluorescence system 21.3 Camera shift The camera shift calibration corrects the shift between the brightfield and monochrome cameras. It is a software-based correction. Measure the camera shift between the two cameras 1. Open the [Manual control] layout. 2. Display the cross hair by selecting the [Cross Hair] entry in the [View] menu. 3. Select the 20x objective. 4. Switch to live mode with the BF observation method selected.
21 Additional calibrations for a fluorescence system 5. Use the stage navigator to move the stage so that the cross of the calibration slide is in the middle of the cross hair. 6. Fine focus with [Ctrl] + mouse wheel. 7. Switch off live mode and change to the BFMono observation method. 8. Switch on live mode again. You might recognize a shift.
21 Additional calibrations for a fluorescence system 9. Switch to the [View] layout, go to the [Measurement and ROI] tab and select either the [Vertical Line] button or the [Horizontal Line] button. 10. Measure the shift between the cross hair and the center structure and note the X and Y values. In this case the monochrome camera is shifted up an to the left. To adjust the shift the cross hair needs to be ‘shifted’ to the right (+X) and down (+Y). 11. Remember these values (+3.86µm in X and +18.
21 Additional calibrations for a fluorescence system 12. In the [Calibrations] dialog box select the [Camera Shift] entry and click the [Edit] button. 13. In the [Camera Shift] dialog box be sure to select the correct objective (20x) and enter the values you measured manually to align the two cameras being used. 14. Click the [OK] button to finish the calibration process.
21 Additional calibrations for a fluorescence system 21.4 Lens Correction On the calibration slide opposite the label area you find an area with checkerboard grids in different sizes. Next to each grid there is a number (red circle in the example image) for the objective magnification from 4x to 60x/100x which has to be used for the calibration. 1. In the [Calibrations] dialog box select the [Lens Correction] entry and click the [Calibrate] button.
21 Additional calibrations for a fluorescence system 2. Select all available objectives. Immersion are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 3. Use the stage navigator to move the VS calibration slide to the correct checkerboard area.
21 Additional calibrations for a fluorescence system 4. Perform an autofocus and proceed with [Next]. 5. Do the same for all other objectives.
21 Additional calibrations for a fluorescence system 6. Finalize the process by clicking the [Finish] button. If you see the message below please check if you are doing the calibration on the correct checkerboard area. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. 21.5 Shading correction BFMono 1.
21 Additional calibrations for a fluorescence system 2. Select the [BF Mono] observation method and proceed with [Next].
21 Additional calibrations for a fluorescence system 3. If you are calibrating an out of the box system (no shading correction was done before) start with the acquisition of the dark current correction image. 4. After the dark current image calibration select all objectives. Immersion objectives are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 5.
21 Additional calibrations for a fluorescence system IMPORTANT The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. 7. Click the [Next] button to start the image acquisition process for the 2x objective.
21 Additional calibrations for a fluorescence system After the acquisition process is finished, the calibration process automatically moves to the next objective. IMPORTANT The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-calibration slide. 8. Proceed with all other objectives in the same way.
21 Additional calibrations for a fluorescence system 9. Finalize the process by clicking the [Finish] button. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. 21.6 Magnification Test Scan 1. In the [Calibrations] dialog box select the [Magnification Test Scan] entry.
21 Additional calibrations for a fluorescence system 2. Click the [Calibrate] button. The magnification test scan for the monochrome camera is identical with the magnification test scan for the color camera. See Magnification Test Scan auf Seite 136. 3. The resulting value should be within a deviation of +/- 0.1 degree. In case the calibration value is not within the standard go back to the [Camera-To-Stage Rotation] calibration and readjust the rotation.
21 Additional calibrations for a fluorescence system 21.7 Shading correction for fluorescence observation methods The shading correction for DAPI, FITC, CY3 and Cy5 can be done on a 'normal' thin, homogenous and large H&E sample using the autofluorescence like in the example below. However also fluorescence samples provided by the customer can be used for shading correction. To perform the shading correction for CY7 a real CY7 stained sample is necessary as CY7 does not show any autofluorescence.
21 Additional calibrations for a fluorescence system 3. Select e.g. the [Cy3] observation method and click [Next]. 4. Always skip the acquisition of the dark current correction image.
21 Additional calibrations for a fluorescence system 5. Select all objectives. Immersion objectives are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 6. It is very important to select in the [Calibration method] group the calibration method [Special]. Only the calibration method [Special] will work on a real sample.
21 Additional calibrations for a fluorescence system 8. Select an area on the slide large enough for a 2x field of view and focus the sample via ‘Ctrl’ + mouse wheel and proceed with [Next]. For objectives equal or higher than 10x try to find a sample area which is quite homogenous. 9. Repeat these steps for all other selected objectives. 10. Continue the shading correction for all other fluorescence observation methods.
21 Additional calibrations for a fluorescence system 21.8 Shading correction for darkfield (DFMono) The darkfield observation method can only be used with 4x and 10x objectives. To perform shading correction for DFMono use the checkerboard sections on the VS-calibration slide. 1. In the [Calibrations] dialog box, select the [Shading Correction] entry and click the [Calibrate] button. 2. Select the [DFMono] observation method and proceed with [Next]. 3.
21 Additional calibrations for a fluorescence system 4. Select only the check boxes for the 4x and 10x objectives. Make sure that the [Shading correction] check box is selected. If necessary, select the [Calibration method] > [Standard] option. Proceed with [Next].
21 Additional calibrations for a fluorescence system The system will activate the live mode with the 4x objective used. 5. Use the [Stage Navigator] tool window to navigate to the 20x checkerboard and focus the sample. 6. Defocus by going down (-100 to 200µm in Z) until you see a homogenous image (no checkerboards) anymore.
21 Additional calibrations for a fluorescence system 7. Click the [Next] button to start the shading correction. 8. Continue in a similar way with the 10x objective. 9. For the 10x objective, go to the 100x/60x checkerboard area and defocus by going down 40µm. 10. Click the [Finish] button to finalize the shading correction. 21.9 Shading correction for PhaseContrast (PH) Prepare a slide with some sample on it for focusing. The rest of the slide should be empty and very clean. 1.
21 Additional calibrations for a fluorescence system 3. Select the [Phase Contrast] observation method. 4. Skip the acquisition of the dark current correction image.
21 Additional calibrations for a fluorescence system 5. Only select the phase contrast objective(s). Make sure that the [Shading correction] check box is selected. 6. Focus on the part of the slide that contains the sample and subsequently search for a very clean area to acquire the shading image.
21 Additional calibrations for a fluorescence system 7. Proceed with [Next]. 8. Finalize the process by clicking the [Finish] button.
21 Additional calibrations for a fluorescence system 21.10 Channel-XY-Shift The channel-XY-shift calibration is only needed if multiple fluorescence filters or dichroic mirrors in the IX3-RFACA are used. As this calibration depends on the customer's specific system configuration and customer's samples, the Olympus employee installing the system should ask for a dedicated sample slide. If such a sample slide is not available, the customer itself can do the calibration at any time.
21 Additional calibrations for a fluorescence system 3. Select the [Calibrate transmission overlay observation methods] (bright field) check box. 4. Put in a customer fluorescent sample which contains stainings for all fluorescent filters that should be calibrated. Use at least the 20x objective, but preferably the 40x objective. 5. Navigate to an area with high contrast and focus. Then click the [Next] button to start the acquisition process. 6.
21 Additional calibrations for a fluorescence system 9. When you have finished, click the [OK] button to save the changes. All calibrations should have a blue tick now.
22 Cleaning the system 22 Cleaning the system 22.1 Cleaning the scanner We recommend cleaning the scanner as required when you can see that it is dirty. ATTENTION Ingress of fluid can damage the devices. An ingress of fluid can cause a short circuit or damage the scanner, individual components of the scanner, the loader, and connected devices. Before cleaning the VS200 system, disconnect it from the power supply.
22 Cleaning the system 6. Clean the following components with a lint free cleaning cloth. Left side of the tray hotel Right side of the tray hotel The bottom panel of the tray hotel 7. For more pronounced marks, you can lightly wet the cleaning cloth with water or a mild solvent-free disinfectant or cleaning agent. 8. Push the tray hotel as far back as it will go to the back panel. 9. Manually move the SCARA robot arm to the left. 10. Clean the gripper only with a lint free cleaning cloth. 11.
22 Cleaning the system Achten sie darauf, dass Sie während der Reinigung der Immersionsobjektive keine Softwarefunktionen ausführen. 1. To clean lenses, remove dust by blowing them with a commercially available blower and wipe them lightly with cleaning paper (or a piece of repeatedly washed gauze). Only if they are stained by fingerprints or oils should you wipe them using cleaning paper slightly moistened with dehydrated alcohol sold at store CAUTION Dehydrated alcohol is highly flammable.
22 Cleaning the system 4. In the [Select the objective to be cleaned] list select the objective to be cleaned. If you have more than one immersion objective, also select the other objectives in the list. 3. To clean the objective use lens cleaning paper. 4. After cleaning the objective click on the [Finish and set Objective status to'clean'] button.
23 Additional software installation 23 Additional software installation 23.1 OlyVIA To install OlyVIA go to D:\OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE\SetupOlyvia and execute the [setup.exe]. 23.2 VS200 ASW Desktop VS200 ASW and VS200 ASW DT cannot be installed on the same customer PC. The installation requires a VS200 ASW DT licence key during the installation. To install VS200 ASW DT on a different PC copy the setup files to the desired PC and execute the setup.exe.
24 Troubleshooting 24 Troubleshooting 24.1 Hardware not available If the [IX3 Nosepiece: Hardware is not available] error message appears, do the following: 1. Close the VS200 ASW software. 2. Switch off the VS200 system! 3. Check cabling of IX3-RFACA. 24.2 "No camera" error If you see the following error messages after switching to the monochrome camera you need to check whether the correct camera is assigned in the observation methods which use a monochrome camera.
24 Troubleshooting 1. Remove the tray and switch to the [Manual control] layout. 2. Use the [Acquire] > [Devices] > [Device Settings] command to open the [Device Settings] dialog box. 3. Select the [VS200L Multi-Sample Holder] > [Tray Types] entry in the tree view. 4. Select the check box [Use Tray] next to the tray type you want to use. 24.4 Image too bright If you see the following error message please adjust VS200 LED lamp voltages. See ORCA camera adjustments auf Seite 91. 24.
24 Troubleshooting screws (size 2.5 mm) indicated in the figure. 3. Use the main power switch to switch on the VS200 system. CAUTION Crush hazard when initializing the VS200 system Switching on the VS200 scanner and starting the VS200 ASW software will initialize all components. As a result, various components begin to move. Gaps open and close when the stage moves. Hands and fingers can get crushed. Make sure that you are not within the stage’s range of movement when it is moving.
24 Troubleshooting 5. Start the VS200 ASW software. 6. Take a tray with a slide in position 3. 7. On your software’s start page, click the [Exchange Trays] button to load the tray. 8. Use the [Load Slide for Calibration] function to load the slide. 9. Make sure that the 2x objective is selected. 10. Starten Sie das Live-Bild in der VS200 ASWSoftware. To do so, go to the [Manual Control] layout. 11. In the [Camera Control] tool window, click the [Live] button to start the live image.
24 Troubleshooting Alternatively, you can also use the [Alt + F6] keystroke. 16. Release the field stop’s focus setting. To do so, loosen the hex screw (size 3 mm) indicated in the figure. 17. Focus the field stop. To do so, insert the hex key into one of the holes indicated in the figure. Move the hex key to the right and to the left to adjust the focus ring. Set the focus so that the edges of the closed field stop are sharp. 18. Tighten the field stop’s focus setting.
24 Troubleshooting 19. Loosen the 2 hex screws (size 3 mm) indicated in the figure. The screws should only have been screwed hand tight. 20. Use the two adjustment screws indicated in the figure to align the bright spot in the center of the cross hair. 21. Lock the field stop’s focus setting. To do so, tighten the hex screw (size 3 mm) loosened before. 22. Open the field stop completely. To do so, turn the adjustment ring shown in the figure to the left as far as possible.
24 Troubleshooting 23. Lock the field stop. To do so, tighten the hex screw (size 3 mm) indicated in the figure. 24. Close the VS200 ASW software and wait until the VS200 scanner has reached its end positions. 25. Use the main power switch to switch off the VS200 system. 26. Montieren Sie den schwarzen Eingreifschutz mittels der sechs Schrauben wieder. 27. Close the door of the VS200 scanner.
25 Proper selection of the power supply cord 25 Proper selection of the power supply cord If no suitable power supply cord has been provided, please select an appropriate power supply cord with a certification mark by referring to the specifications and the table below. Olympus is not responsible for damage caused by the use of uncertified power cords with Olympus devices.
25 Proper selection of the power supply cord Table 1 - Certification marks for power cords The power cord has to have a certification mark from one of the bodies listed in table 1 or it must use a cable that has been tested by a body that is listed in table 1 or table 2. The plug has to have at least one certification mark shown in table 1.
25 Proper selection of the power supply cord Table 2 - HAR Flexible cables Certification body Printed or embossed harmonization mark (on the plug or cable insulation) Comité Électrotechnique Belge (CEBEC) CEBEC VDE Verband der Elektrotechnik Elektronik Informationstechnik e.V. Union Technique de l’Électricité (UTE) USE Istituto Italiano del Marchio di Qualità (IMQ) IEMMEQU British Approvals Service for Cables (BASEC) BASEC N.V.
26 Declarations of conformity and disposal 26 Declarations of conformity and disposal 26.1 CE Conformity (Europe) This system complies to the requirements of the following European directives: Low Voltage Directive 2014/35/EU EMC Directive 2014/30/EU Machinery Directive 2006/42/EC Radio Equipment Directive (RED) 2014/53/EU This system complies with the requirements of Directive 2014/30/EU concerning electromagnetic compatibility according to Standard IEC/EN61326-1.
26 Declarations of conformity and disposal If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures: 1. Reorient or relocate the receiving antenna 2. Increase the separation between the equipment and receiver 3. Connect the equipment into an outlet on a circuit different from that to which the receiver is connected 4.
26 Declarations of conformity and disposal FCC Part 15.19(a) [interference compliance statement], unless the following statement is already provided on the device label: This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. 26.7.
27 Support 27 Support Delete this text and replace it with your own content.
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