DRAFT User Guide Version 1.1 For research use only. Not for use in diagnostic procedures.
Table of Contents Contact Information and Legal..................................................................... 2 Section 1: General Information . . ...................................................................... 3 Section 2: Safety and Compliance ............................................................. 3 Section 3: Operating Conditions ................................................................. 4 Section 4: Components ..................................................................
Contact Information Miroculus, Inc. 458 Brannan St. San Francisco, CA 94107 techsupport@miroculus.com PHONE: (415) 287-0505 WEB: miroculus.com EMAIL: Legal All Rights Reserved. No part of this publication may be reproduced, transcribed, or transmitted in any form, or by any means, electronic or mechanical, including photocopying and recording, for any purpose other than the purchaser’s use without written permission of Miroculus, Inc.
Section 1 • General Information INTENDED USE The Miro Canvas is a benchtop digital microfluidics platform that delivers custom low-throughput workflow automation for academic, research, and pharmaceutical life science laboratories. The Miro Canvas is for Research Use Only (RUO).
SYMBOL TABLE European Conformity UK Conformity Assessed High Voltage Warning or Caution OPERATING PRECAUTIONS • Instrument is only to be operated by personnel who have read the User Guide in full and are using proper personal protective equipment. • Voltage applied during use. Follow all instructions when loading the instrument. • Use care when loading the cartridge on the instrument electrode board. • Handle liquids near the instrument with extreme caution. • Do not overfill reagent reservoirs.
INDUSTRY CANADA COMPLIANCE This device complies with Industry Canada licence-exempt RSS standard(s). Operation is subject to the following two conditions: (1) this device may not cause interference, and (2) this device must accept any interference, including interference that may cause undesired operation of the device. Cet appareil est conforme avec Industrie Canada exempts de licence standard RSS (s).
INSTRUMENT LABELING Model: Canvas 1.0 Miroculus, Inc. 458 Brannan St. S/N: San Francisco, CA 94107 USA P/N: M-01-0001-001 Caution: Voltage applied during use Attention: Voltage élevé durant utilisation Research Use Only Input: 24V 3A C 61010 This device complies with part 15 of the FCC Rules.
Section 4 • Components Lid Manifold Clamp Electrode Board Clip Touch Screen INSTRUMENT AND ELECTRODE BOARD INSTRUMENT (SIDE) 4 • Miro Canvas • User Guide INSTRUMENT (FRONT)
Section 5 • Installation 1. INFRASTRUCTURE REQUIREMENTS • 120V 3-prong outlet • Networked computer with Google Chrome (browser version 75.x or later) • Local area network connectivity supporting a speed of 100 Mb/s via RJ-45 shielded Ethernet cable or 802.11a/b/g/n compatible Wi-Fi wireless network • Please note that, at this time, we cannot connect to Wi-Fi networks requiring captive portal login (opening of a page which requires input of user name and/or password).
iii. Power up the instrument by pressing the black power button on the back of the Canvas. The LED indicator bar will change to blue as the instrument initializes. 1. The instrument may take a few minutes to power up. iv. Use the touchscreen Settings menu to: 1. Connect to a network a. To use an Ethernet connection (recommended for stability), plug in an Ethernet cord and toggle off Wi-Fi on the touchscreen Settings menu. b.
2. PROCEDURE • Tube Recommendations The table below shows tubes that are known to be both compatible and incompatible with Miro Cartridge. This list is not comprehensive. SUPPLIER Compatible Incompatible CAT. NO. PRODUCT DESCRIPTION Applied Biosystems N8010580 MicroAmp 8-Tube Strip; 0.2ml Axygen PCR-02-L-C Maxymum Recovery PCR Tube; 0.2ml Axygen PCR-02-C PCR Tubes with Flat Cap; 0.2ml Eppendorf NA 86403 Tube Microcentrifuge DNA LoBind;1.
• Instrument Loading i. Power up the Miro Canvas by pressing the black power button on the back of the instrument. ii. Follow the directions on the touchscreen to load the instrument. 1. If necessary, first use Miro Palette to send the protocol to the instrument. 2. On the instrument touchscreen, select the user and the desired protocol. iii.
b. To seal the reservoir, fully press the blue cap onto the reservoir. c. Reagents loaded into reservoirs cannot be automatically detected as inlet reagents can. Press Continue on the touchscreen to advance to the next reservoir. iii. Once all reagents are loaded, follow the touchscreen instructions to start the run. a. Examine the manifold on the underside of the lid to ensure it is clear of debris. b.
Section 7 • Maintenance and Troubleshooting 1. MAINTENANCE • No periodic (weekly, monthly, annual) maintenance is required to be performed by either the end user or a service professional. It is recommended to run Miro Diagnostics weekly. • If instrument malfunction is suspected, instrument functionality can be tested by running Miro Diagnostics. Contact Miroculus Technical Support with any questions regarding suspected instrument, cartridge, or reagent malfunction.
RUNNING A PROTOCOL On-screen Error: Voltage Voltage is not detected and run cannot proceed. Please ensure cartridge clamp is engaged, press cancel, and restart the run. On-screen Error: Instrument Not Level Using the provided leveling plate, adjust the feet of the instrument to ensure the bubble is in the center, indicating a level state. If error persists, please contact Miroculus Technical Support.
On-screen Error: Dispensing System The instrument is not functioning as expected. Run has failed and an attempt will be made to recover your sample. Follow the directions on the screen. Keeping the cartridge clamp engaged, examine the manifold on the underside of the lid to ensure it is clear of debris. If any debris is found, remove it from the manifold and restart the run. Cartridge clamp will not fully close (clip does not engage) Debris in the clamp bed can interfere with clamp closing.
Bubbles in pipette tip prior to loading Wait for the bubbles to resolve before dispensing into the inlet. On-screen Error: Droplet Mobility Failure The instrument is not functioning as expected. Run has failed and an attempt will be made to recover your sample. Follow the directions on the screen. Input sample quality may be lower than expected. Examine input DNA using a Bioanalyzer or similar instrument. Adapters may have undergone too many freeze-thaw cycles.
NETWORK CONNECTION INDICATORS Green dot on touchscreen Connected to network, internet enabled Yellow dot on touchscreen Connected to network, No internet access. Red dot on touchscreen No network or internet connected.
