MUSCLE STRIP MYOGRAPH SYSTEM MODEL 820MS User Manual Version 1.
MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL
MUSCLE STRIP MYOGRAPH SYSTEM MODEL 820MS TRADEMARKS PowerLab® and LabChart® are registered trademarks of ADInstruments Pty Ltd. The names of specific recording units, such as PowerLab 4/35, are trademarks of ADInstruments Pty Ltd. Pentium is a registered trademark of the Intel Corporation. Windows, Windows 95, Windows 98, Windows ME, Windows NT, Windows 2000, Windows XP, Windows Vista and Windows 7 are registered trademarks of Microsoft Corporation.
CONTENTS Trademarks.........................................................................................................................................................................................3 Introduction ........................................................................................................................................................................................5 Safety ....................................................................................................
INTRODUCTION The Muscle Strip Myograph System - 820MS represents the first state-of-the-art 4-channel myograph system for muscle strips of up to 15 mm in length. DMT developed this system for simultaneous measurement of up to 4 isolated muscle preparations, mounted as strips, between clamp supports. Several types of mounting supports exist, depending on the user’s preferences. Customized mounting supports can be made upon request.
SAFETY The Muscle Strip Myograph System - 820MS has been designed for use in teaching and research only. It is not intended for clinical or critical life-care use and should never be used for these purposes. The 820MS also should not be used for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap. The following are other safety concerns of note: • Do not open the unit; the internal electronics pose a risk of electric shock. • Do not use this apparatus near water.
EMC / EMI This equipment has been tested and found to comply with the limits for a Class B Digital device, pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference in residential installations. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications.
CERTIFICATE OF CONFORMITY DMT A/S, Skejbyparken 152, 8200 Aarhus N., Denmark, hereby declares its responsibility that the following product: Muscle Strip Myograph System Model 820MS is covered by this certificate and marked with CE-label conforms with the following standards: EN 61010-1:2001 EN 61010-1/Corr.1:2003 EN 61010-1/Corr.1:2003 Safety requirements for electrical equipment for measurement, control, and laboratory use Part 1: General requirements.
ABOUT THIS MANUAL This manual contains a complete list of procedures that describe how to install, maintain and use the Muscle Strip Myograph System – 820MS. Chapter 1 provides an overview of the construction and basic features of the Myo-Interface and the myograph unit. Chapter 2 describes step-by-step how to set-up the complete Muscle Strip Myograph System – 820MS including accessories. Chapter 3 is a complete manual to the Myo-Interface.
UNPACKING THE MYOGRAPH SYSTEM Take a few minutes to carefully inspect your new Muscle Strip Myograph System - 820MS for damage which may have occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued soon as possible. If the packing material appears damaged, please retain it until a possible claim has been settled.
CHAPTER 1 - SYSTEM OVERVIEW 1.1 Myo-Interface front panel Myo-Interface display Valve buttons Heat indicator Power indicator Figure 1.1 - Interface Front Panel with 820MS chambers 1.2 Interface Rear Panel 4 Transducer connections Gas regulator (needle valve) RS 232 Port for serial connection to PC Gas input Temperature probe 4 Recorder outputs Power connector Vacuum input ON/OFF switch USB output Figure 1.
1.3 Muscle Strip myograph unit Connection to Myo-Interface Micrometer Figure 1.3 Muscle Strip myograph unit Myograph clamp support connected to micrometer Force transducer pin Myograph clamp support connected to force transducer Figure 1.
CHAPTER 2 - SETTING-UP 2.1 The complete Muscle Strip Myograph System - 820MS DMT CS200 Pulse/Train Stimulator (optional) PowerLab data acquisition system (optional) PC data acquisition and analysis software (optional) Myo-Interface front panel PC USB connection Connection to oxygen supply BNC Cables Myo-Interface rear panel Vacuum pump (optional) Suction bottle (optional) Figure 2.1 The complete Muscle Strip Myograph System - 820MS 2.
NOTE: THE NEEDLE VALVES NEED TO BE GREASED (USING THE GREASE FOR THE LINEAR SLIDES) AND TURNED AT REGULAR INTERVALS TO PREVENT THEM FROM STICKING OR PERMANENTLY FREEZING. 3. Vacuum Connection The system has a built-in manifold with separate valves that allows each chamber to be drained individually. After connecting the vacuum source at the back of the Interface, the vacuum pipes need to be inserted into the chambers in order for this feature to work properly.
CHAPTER 3 - THE INTERFACE MENUS Chapter 3 is a complete manual for the 820MS Interface. This chapter contains a detailed description of how to navigate the touch-screen menus and how to use the special features of the 820MS myograph. The 820MS system can be used in 2 different modes. The first mode is the Calibrated Mode, where the signal from the transducers is filtered and conditioned before the signal is routed to the BNC outputs in the back of the interface for collection by a data acquisition system.
3.2 Interface Menus – Calibrated Mode In calibrated mode, the internal amplifier is engaged and signals from the transducer are filtered and conditioned before the signal is sent to a data acquisition system via the BNC outputs on the rear of the interface. The total voltage output from the BNC connectors is 2.5 volts. Power-Up Screen DMT820 MYOGRAPH After turning on the 820MS Interface, an “Introduction” screen appears. The system is auto-calibrating the A/D converters while this screen is displayed.
Zero Menu This menu is used to zero the output of the transducers. When using a data acquisition program like LabChart by AD Instruments ®, using this feature will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre-load tensions placed on the mounted vessels. The channels can be changed individually by pressing “SELECT” or all at once by pressing “ALL”. Pressing “ENTER” will execute the zero function. SET FORCE TO ZERO Force chamber 1: -2.
1. Force calibration NOTE: EVERYTIME A FORCE CALIBRATION IS PERFORMED THE MEASUREMENT RANGE IS SET TO DEFAULT 200 mN. SET MEASUREMENT RANGE AFTER THE FORCE CALIBRATION. Enter the FORCE CALIBRATION sub-menu to perform the calibration procedure.
Step 3 Initiates the heating process for the chambers. In order to perform an accurate calibration the transducers must be heated to the desired experimental temperature to accommodate heat-induced expansion of the electronic parts in the transducer. Otherwise inaccurate readings and transducer drift may occur. CHAMBER 1 CALIBRATION Step no.: 1 2 3 4 5 6 Turn the heat on. Wait until the temperature is stable. Temperature set-point : 37.0 °C Probe temperature: 36.
2. Valve delay: Pressing “VALVE DELAY” in the SETTINGS menu will allow the user to modify the time duration that the vacuum valves stay open for washes. Factory default is set at 1 second, but 1 second is not enough time to completely empty a chamber with even as small a volume of 5 ml. EMPTY VALVES DELAY Chamber 1: 5 Sec. SELECT Chamber 2: 6 Sec. SELECT Chamber 3: 6 Sec. SELECT Chamber 4: 5 Sec. SELECT ALL Pressing “SELECT” next to any given channel will cause the line selected to turn blue.
4. Measurement Range: The MEASUREMENT RANGE sub-menu in SETTINGS determines the maximum force capacity of the transducer. The factory setting is 200 mN, but the transducer capacity can be changed to 400 mN, 800 mN or a maximum of 1600 mN of force detection, depending on the size of the vessel used. The “SELECT” and “ALL” functions are the same in this menu as previously described for the “VALVE DELAY” menu. Pressing “ENTER” will store the numbers in memory for future experiments. 5.
II. RECORD OUTPUT SELECT The RECORD OUTPUT SELECT option allows the end-user to select the signal output from the transducers. This option deals with how the signal from the transducers to the BNC outputs are handled and routed. The 2 options are: RECORD OUTPUT TYPE SELECT Record output is: Calibrated SELECT Record output is: Direct SELECT 1. Record output is: Calibrated 2. Record output is: Direct To select the type of output, press SELECT. The text selected will appear in blue.
After a few seconds, the DMT 820MS Direct Force Output display will appear. When in Direct Mode, the ACTUAL FORCE display will not be shown, as is the case when the system is in the Calibrated Mode (see “3.2 Interface Menus – Calibrated Mode” on page 16).The force readings will only be available in the data acquisition system connected via the BNC outputs. DIRECT FORCE OUTPUT Many of the menus in the Direct Mode will be similar to those in the Calibrated Mode.
CHAPTER 4 - THE MUSCLE STRIP MYOGRAPH UNIT Chapter 4 contains a complete explanation of how to calibrate and maintain the 820MS myograph to ensure the equipment is always performing at peak performance. This Chapter also covers the mounting supports and how to mount a muscle strip in the 820MS chamber. 4.1 Calibration of the force transducer As a part of the general maintenance of the myograph, DMT recommends that the myograph is weight-calibrated at least once a month.
Muscle with relative large contractile forces (>200mN) For muscle strips of interest producing relatively large contractile forces, the following calibration procedure is recommended. For example, muscle strips that may potentially be producing forces around 300 mN, then the FORCE REC. OUTPUT and MEASUREMENT RANGE options should be set appropriately (see “3.2 Interface Menus – Calibrated Mode” on page 16). As an example, FORCE REC.
4.2 Checking the force transducer The myograph force transducer is a strain gauge connected to a Wheatstone bridge. The force transducers for each chamber are housed in a separate, protective compartment (see figure 4.3 below).
4. The replacement transducer will be shipped with the new transducer inside a new transducer house. 5. Place a VERY small amount of vacuum grease (clear or whitish grease) around the bottom inside of the transducer housing to seal the transducer housing when put back in place. An arrow in figure 4.5 below indicate the place that the grease needs to be applied. 6.
4.4 Myograph Maintenance The Muscle Strip Myograph System - 820MS is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections are read carefully and that the instructions are followed at all times. Myograph chamber tubing To prevent the tubing from becoming blocked with buffer salt deposits after an experiment, remove the chamber cover from the Myograph Chamber.
4.5 Cleaning the myograph DMT strongly recommends that the myograph chambers and surrounding areas are cleaned after each experiment. At the end of each experiment, use the following procedure to clean the myograph chambers and supports: 1. Fill the myograph chamber to the edge with an 8% acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build-up. Use a cotton-tipped applicator to mechanically clean all chamber surfaces. 2.
CHAPTER 5 - THE MOUNTING SUPPORTS AND MOUNTING IN THE 820MS CHAMBER Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the myograph chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction. They are also designed to effectively “grab” tendon on whole striated muscle preparations without experiencing “slippage” during a whole striated muscle contraction. 1. Starting position. 2.
APPENDIX 1 - SYSTEM SPECIFICATIONS Technical specifications Vessel size: Chamber: Chamber material: Chamber volume: Chamber suction: Chamber cover: Chamber gassing: Force range: Force resolution: Weight calibration: Heating: Temp. range: Temp. resolution: Temp. probe: Output reading: Analogue output: Voltage: Ambient temp.: >3 mm - 19 mm Four individual chambers Acid-resistant stainless steel Max.
Aalborg Hospital South • Academic Medical Center Amsterdam • Academy of Sciences of the Czech Republic • Actelion Pharmaceuticals Ltd • Ahmadu Bello University • Akzo Nobel/Organon • Albert Einstein College of Medicine • Albert-Ludwigs-Universität Freiburg • Arete Therapeutics • Aarhus Kommunehospital • Arizona State University • Asterand UK Ltd.
