MultiGene Mini Thermal Cycler TC020-24 /TC020-24-230V TC050-18 /TC050-18-230V Operation Manual Ver.1.
CONTENTS CHAPTER 1 SPECIFICATIONS ...................................................................................................... 1 1 Normal Operating Conditions .................................................................................................. 1 2 Transportation and Storage Conditions................................................................................... 1 3 Basic Specifications..........................................................................................
CHAPTER 1 SPECIFICATIONS This chapter describes the MultiGene Mini Thermal Cycler’s operation, transportation and storage conditions, basic parameters, performance and functions. 1 Normal Operating Conditions Ambient operating temperature: Relative humidity: Power supply: Note: 10°C ∼ 30°C ≤70% ~100-120V, 200W, 50/60Hz ~220-240V, 200W, 50/60Hz Before powering on, please confirm that the above operating conditions are satisfied. The unit must be plugged into a grounded outlet.
Specifications Temperature range: Maximum Heating rate: Maximum Cooling rate: Temperature Resolution Block Temperature accuracy: Temperature display accuracy: Block temperature uniformity: Heated lid work temperature range: 4ºC～99ºC ≥5.0ºC/sec(max) ≥4.0ºC/sec(max) ± 0.1°C ≤±0.3ºC ≤±0.5ºC ≤±0.
CHAPTER 2 Features This chapter covers the MultiGene Mini Thermal Cycler’s mechanics, the keyboard and each key’s function, and preparations before power-on. 1 Description 1 2 3 4 5 6 7 ① Heated lid & Block ② LCD Display ③ Keyboard ⑤ Power switch ⑥ Power connecter ⑦ Fuse Socket Note: ④ RS232 interface 1 The fuse specifications are as follows: 250V:2.5A / 125V:5A, Ø5×20mm. The fuse should be replaced with one that meets these specifications.
2 Keyboard Diagram 3 Key Functions “Numbers & Letters”, select the letter by pressing the key until your selection appears on the screen. “CE”，can be used to change “+, -” when editing temperature and time. “Right/Left”，moves the cursor one position to the left or right during file parameter setting. “Up/Down”，moving the cursor one position up or down during file parameter setting. “Enter”，press it to accept the present parameter settings displayed on the screen.
4 Inspection before Power-up Before switching the power on, please ensure that: 1) Supply voltage matches the voltage rating label on the instrument. (refer to Chapter 2); 2) The plug has been inserted into the power socket; 5 Power-on Procedures Turn on the power (i.e. turn the power switch to position ‘1’), the MultiGene Mini Thermal Cycler will beep 4 times, and the LCD will display ‘ ’. This means the device is self-testing, which takes about 1-2 minutes.
CHAPTER 3 OPERATION GUIDE There are eight menu selections for the MultiGene Mini Thermal Cycler. In this chapter, we will explain operation, for example, how to edit, access, modify, delete, or run a protocol file and how to set parameters.
If the default user does not have a password, press “Run” to run the default file. You will be prompted to enter sample volume(if the unit is set for Tube Mode) in the pop up window. If the unit is set for block mode, the unit will begin running the default file. If the default user does have a password, you will be prompted to enter it before you can run the default file. Password Prompt Hotlid=off Enter password - 2009-04-01 10:05:00 Labnet MultiGene Mini Version: 2009-1.
user. Shift to the File Name Column, using the “right” arrow button, then select the user’s file using the up/down keys. File List Menu Press “Edit” to edit the file Hotlid=off 2008-07-28 10:05:00 indicated by the cursor. No. User File Name Save Time Press “New File” to create a new 1 Jeff1* Amp1 2008-07-28 2 Mike2 Amp2* 2008-07-28 file. 3 Sue3 Amp3 2008-07-28 Press “Delete” and you will be prompted “Do you want to delete the file? Please select ‘F3’ or ‘F4’”, Select（Delete） or （Back）.
Use the key pad to input the sample volume. Press “Back” to go back to the file list menu. Press “Run” to enter file running menu. Note： The symbol “*”at the top of User and File indicates the default User and file. The Default User name is LABNET and the default file is 1. The default file cannot be renamed, but it can be edited. File Editing Screen In this screen, you can add and edit Hotlid=020C 2008-11-01 10:05:00 cycles and segments of the file. A File 100 is comprised by cycles and segments.
Seg. 1 section 1 Cycle1 ×** From ** to ** File Seg. 2 section 2 Cycle2 ×** From ** to ** ·· · ·· · section 5 Cycle5 ×** From ** to ** Seg. 16 Relation of cycle and segments in a file File Saving Screen In this screen use the keypad to enter the file name. Press “Enter” to move the cursor down one position. Select User and File Name through the popup character selection box. Click left button to delete characters while clicking right button to move the cursor.
Enter the sample volume. Press “Back” to go back to the file save menu. Press “Run” to start running the file. In the file save menu, if you press “Run” without pressing “Save”, the system will display “The modifications will be lost after running. Do you still want to Hotlid=off 2007-07-28 10:05:00 run?” abc Press “No” to go back file save interface. User： Labnet i Press “Yes” to enter the file running menu and sample volume File Name： Num1 1 input screen.
1.2 How to Set System Parameters In the main screen press “System” to enter the system parameter settings menu. Press “Enter” to move the cursor to the next item. This operation is followed by the symbol “ ”. Press “Up/Down” to scroll to an option, and information can be entered by using the keyboard. Press “Right/Left” to move cursor’s position. Press “Back” to go back to the main menu. Press “Save” to save parameters.
“Tube mode” controls the cooling & heating rate of the block, inconsideration of the sample temperature changing at a slower rate. In tube mode, there is an overshoot after reaching the set temperature, which allows the sample to reach the set temperature faster, and will help the stay at the set temperature for a longer period. (Please refer to the following figure: relation between block temperature and reagent temperature.) Block Reagent 1.2.
CHAPTER 4 TROUBLE SHOOTING 1 Failure Analysis and Processing Procedures No. Phenomenon Failure Analysis Disconnected power supply 1 2 3 4 No display and the two beeps missed when power on Abrupt change in heating speed or bad temperature control accuracy Significant change in cooling speed, or unavailable temperature below ambient Heating and cooling terminated Bad fuse Troubleshooting Check power supply for correct connection Replace it (250V 2.
2 Error Messages No. Error Message Possible Reasons 1 Error Message when running “Please contact the distributor”， with “AD1213 failure” The microchip can not communicate with the AD1213. AD1213 broken or circuit board trouble. 2 Error Message when running “Please contact the distributor.”， with “Heatsink temperature is too high” Heat sink temp. higher than 70℃，the temperature sensor is broken, there is a short circuit or the fan is not working.
APPENDIX 1 Wiring Diagram (1 of 2) AC input_ 220-240VAC 50/60Hz U5 AC Line Filter PCB1 BYQ904102000110_HOTLID HOTLID 123 N N I EI LPN LID-J1-1_SEN LID-J1-2_SEN LID-J1-3_HEATER+ LID-J1-4_HEATER+ LID-J1-5_HEATER+ LID-J1-6_HEATERLID-J1-7_HEATERLID-J1-8_HEATER- E LPN 564 FUSE1 5*20mm_ 2.
Wiring Diagram (2 of 2) Hotl id PCB1 BYQ904102000110 Rated Voltage input: AC100-120V 50/60Hz1 N2 3 N HOTLID I EI LPN U5 Filter INLET E LPN 564 FUSE1 5*20mm_ 5A N Powerboard PCB3 BYQ504104200000U SW1 PE J1-8_AUX HEATER J1-7_AUX HEATER J1-6_SEN-B LOCK J1-5_SEN-B LOCK J1-4_SEN-S INK J1-3_SEN-S INK J1-2_TEJ1-1_TE+ BLOCK Switch L N C C A A PCB2 BYQ904101000010 LID-J2-1_SEN LID-J2-2_SEN LID-J2-3_HEATER+ LID-J2-4_HEATER- FUSE2 5*20mm_ 5A L Block LID-J1-1_SEN LID-J1-2_SEN LID-J1-3_HEATER+ LID-J
APPENDIX 2 Parameter Values Item 1 Parameter Temp(ºC) 2 Time 3 Ramp(C/S) 4 +Temp 5 +Time Cycle1 ×00 6 From 00 to 00 Scale Temp. Control point Time of Temp. Control（mm:ss） Ramping Rate （ºC/Second） Temp. Gain of Every Cycler Time Gain of Every Cycler（mm:ss） Section Numbers Cycler Numbers from beginning to the end The start segment of the section The end segment of the section. Gradient Temp. Difference（ ） Range Note 4.0~99.0 --:-（set the value before “：”through “Function” key.） 00：00~59：59 0.
block block reagent reagent Image 2 Image1 19
Limited Warranty Labnet International, Inc. warrants that this product will be free from defects in material and workmanship for a period of 18 months from date of purchase. This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied instruction manual. Should this product require service, contact Labnet International, Inc.’s Service department at 732-417-0700 to receive a return authorization number and shipping instructions.
