® ® An IRIS Company Urinalysis System OPERATOR'S MANUAL
Table of Contents OPERATOR’S MANUAL Please read the entire Operator’s Manual prior to operating the CenSlide Centrifuge. This manual includes easy-to-follow instructions, technical information about the CenSlide 2000 Urinalysis System, maintenance instructions, and a simple trouble shooting guide. TABLE OF CONTENTS Product Overview 1.0 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1 1.1 CenSlide 2000 Centrifuge . . . . . . . . . . . . . . . . . . . .1-2 1.2 CenSlide Tubes .
Table of Contents OPERATOR’S MANUAL Special Topics - Continued 3.0.2 Staining Characteristics . . . . . . . . . . . . . . . . . . .3-2 3.1 Confirmatory Protein Assay . . . . . . . . . . . . . . . . . . .3-4 3.2 Differentiating red blood cells from white blood cells . . . . . . . . . . . . . . . . . . . . . . .3-5 3.3 Microscope Objectives . . . . . . . . . . . . . . . . . . . . . . .3-6 3.3.1 Background . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6 3.3.
Product Overview OPERATOR’S MANUAL The CenSlide 2000 Urinalysis System is a closed system used in performing microscopic examinations of urine. For In vitro diagnostic use. 1.0 General The CenSlide 2000 Urinalysis System reduces exposure to biohazardous materials and helps laboratories control inherent variations in conventional microscopic analysis by standardizing urine sediment volume, centrifuge conditions, and technician technique.
Product Overview OPERATOR’S MANUAL The CenSlide 2000 Centrifuge is microprocessor-controlled to automatically spin CenSlide tubes for a preprogrammed time and speed. At the end of the centrifuge cycle, the urine sediment is redistributed evenly across the viewing area of the CenSlide tube, completely ready for microscopic analysis. The CenSlide tube inserts directly into a CenSlide Tube Holder which is located on the microscope platform.
Product Overview OPERATOR’S MANUAL 1.2 CenSlide Tubes Viewing Area The patented CenSlide tube (Figure 1.3) is a combination tube and microscope slide. The CenSlide tube reduces processing time, and eliminates errors associated with decanting the supernatant and resuspending the microscopic particles thereby increasing precision and accuracy. Cap Figure 1.
Product Overview OPERATOR’S MANUAL 1.3 CenSlide 2000 Centrifuge Specifications Power Requirements Model Power Supply Mains M401-12 . . . . . . . . . . . . . . .120 VAC, 60 Hz, 26 W (Product No. 2C-9000-001) M401-22 . . . . . . . . . . . . . . .90-240 VAC, 50/60 Hz, 26 W (Product No. 2C-9000-22) Centrifuge Motor . . . . . . . .24 VDC, Brush Type, Solid State Microprocessor Controlled Lid and Bowl . . . . . . . . . . .One piece molded Nylon plastic Centrifuge Cycle Ramp to 1350 rpm ±10% . .
Product Overview OPERATOR’S MANUAL 1.4 CenSlide Tube Specifications Description Disposable, non-sterile, shatterproof, polypropylene plastic molded tube with clear viewing area incorporated. Tubes are packaged with recessive leak-resistant caps in shrinkwrapped boxes. Dimensions “D” shaped taper, 110mm long x 21mm wide Viewing Area Dimensions 7.9mm (w) x 10.7mm (l) x 0.25mm (h) Total Volume 5.
Operation OPERATOR’S MANUAL This chapter covers the installation and general operation of the CenSlide 2000 Urinalysis System. 2.0 Installing the Censlide 2000 Centrifuge After removing the CenSlide 2000 Centrifuge from its box, place it on a level working surface close to an electrical outlet. The CenSlide 2000 Centrifuge operates on 24 volts, supplied by an external power supply module. Plug the power Figure 2.
Operation OPERATOR’S MANUAL 2.2 Precautions The CenSlide 2000 Centrifuge is designed to concentrate urine sediment in CenSlide tubes for diagnostic testing. Therefore, use of the CenSlide 2000 Centrifuge places a responsibility upon administrative personnel to ensure adequate training of operators as to the safe and effective use of the centrifuge. 2.3 Hazards The CenSlide 2000 Centrifuge is not explosion-proof and should not be used in a potentially explosive atmosphere. 2.
Operation OPERATOR’S MANUAL 4. If stain is required, add it to the sample after the chemistry test strip has been removed and read (Figure 2.2). 5. Place the cap on the CenSlide tube and push down firmly until it clicks and/or its rim is flush with the top of the CenSlide tube. 6. Holding the CenSlide tube just below the cap, align it so that the flat side of the CenSlide tube conforms to the flat side of the slot in the rotor head (Figure 2.3).
Operation OPERATOR’S MANUAL 2.6 Centrifuge Operation 1.Press the green “START” button on the control panel of the CenSlide 2000 Centrifuge (Figure 2.4). The amber “CYCLE” light will flash, indicating that the centrifuge is currently in operation. Figure 2.4 For safety purposes, the lid will not open while the rotor is turning. Microprocessor technology is used to monitor the speed and time to assure accuracy.
Operation OPERATOR’S MANUAL 2.7 Sample Analysis 1. Open the lid and remove the CenSlide tubes. 2. Place the CenSlide tubes into the StakRak™ horizontally (Figure 2.5). Allow the urine sediment in the tubes to settle for at least one minute prior to viewing. Figure 2.5 3. Place a CenSlide Holder onto the microscope stage. 4. Place a CenSlide tube into the CenSlide Holder and position its viewing area under the microscope objective (Figure 2.6). Figure 2.6 5.
Operation OPERATOR’S MANUAL 2.8 Quality Control There are a number of commercially available urine controls that contain white blood cells, red blood cells, and crystals that may be used for daily verification of the CenSlide Urinalysis System. The quality control procedures established for your laboratory should be followed.
Special Topics OPERATOR’S MANUAL This chapter covers special procedures, technical specifications, and references. 3.0 Staining The recommended stain for use with the CenSlide 2000 is a modified Sternheimer-Malbin stain. This stain is commercially available, call StatSpin for source (800-782-8774). This stain, when used properly, provides increased contrast and specific staining of urinary sediment. This makes recognition of some formed particles, especially casts, easier. 3.0.
Special Topics OPERATOR’S MANUAL Warning: This product will stain fabrics. Use caution when dispensing. 5. Place the cap on the CenSlide tube and push down firmly until it clicks and/or its rim is flush with the top of the CenSlide tube. Invert two to three times to evenly mix the specimen with the stain. 6. Refer to Chapter 2, pages 2-3 through 2-5 for instruction on completing the preparation and reading of CenSlide tubes. 3.0.
Special Topics Bladder Epithelial Cells: OPERATOR’S MANUAL Round cells with a blue nucleus and a large encircling border of pale blue cytoplasm. Squamous Vaginal / These are ragged and irregularly Urethral Cells: shaped cells with orange-to-purple nuclei and extensive blue or purple cytoplasm. Hyaline Casts: Hyaline casts appear pink to light purple with uniform matrices. RBC Casts: They appear pale pink with unstained or pale lavender red blood cells enmeshed within their matrix.
Special Topics OPERATOR’S MANUAL Granular casts: They appear pale pink with granules staining light purple (fine granular) or dark purple (coarse granular) enmeshed within their hyaline matrix. Fatty Casts: They appear pale pink with highly refractile unstained globules within their hyaline matrix. Trichomonas Vaginalis: A dark purple nucleus with a large encircling border of light purple matrix. Budding Yeast: Stain a light to medium purple. Mucous: Stain a light to medium purple.
Special Topics OPERATOR’S MANUAL 3.1 Confirmatory Protein Assay (cont.) 3. After centrifugation, remove the CenSlide tube from the centrifuge and decant the supernatant into a clean, clear tube. Be very careful when decanting the supernatant not to disturb the sediment at the bottom of the CenSlide tube. 4. Add an equal volume of sulfosalicylic acid (Exton’s Reagent) to the decanted supernatant and mix. 5. Grade for cloudiness. 3.
Special Topics OPERATOR’S MANUAL 4. Mix the acetic acid into the CenSlide tube’s viewing area by bending it five times, hand flicking it five times, and then bending it five more times. 5. Place the CenSlide tube in the StakRak™ horizontally and allow the urine sediment to settle for two minutes prior to microscopic examination. Note: Bending and hand flicking the viewing area causes an increase in the number of cellular elements in the viewing area.
Special Topics OPERATOR’S MANUAL 3.3.2 Non American Optical (Non-AO) Microscopes After 1979, the Deutsche Industrial Norm (DIN Standard) was accepted by most microscope manufacturers. This standardization of objective lengths solves the problem of the focusing distance when DIN Standard High Resolution Long Working Distance objectives are used. Microscopes equipped with 40x DIN Standard objectives may hit the CenSlide tube’s viewing area when it is rotated from the 10x objective to the 40x objective.
Special Topics OPERATOR’S MANUAL On an unmodified American Optical model 110 series microscope, all shims will be required. For a modified American Optical model 110 series microscope, it may be necessary to remove some of the shims. Remove the shims, one at a time, starting with the thinnest, until it is possible to go back and forth between the 10x and 40x with minimal amount of refocusing. Contact StatSpin Technical Service if assistance is required. 3.3.
Maintenance OPERATOR’S MANUAL This chapter explains how to take care of your CenSlide tubes and CenSlide 2000 Centrifuge. If you have any questions on how to use your CenSlide Urinalysis System, simply call the StatSpin Hotline at: 800-782-8774. A StatSpin representative will gladly answer your questions, or talk you through any procedures. 4.0 Cleaning the Censlide 2000 Centrifuge For Urine Spills Clean up urine spills inside the CenSlide Centrifuge bowl with a cloth dampened with 5% bleach.
Maintenance OPERATOR’S MANUAL 4.2 Censlide 2000 Centrifuge Calibration The CenSlide 2000 Centrifuge uses microprocessor technology to monitor the speed (rpm) and timing to ensure the accuracy of each test. No initial rpm calibration is required. The rpm can be independently checked with a photoelectric tachometer available from many sources. To check the rpm: 1. Insert six (6) water-filled CenSlide tubes into the rotor. 2.
Trouble Shooting Guide OPERATOR’S MANUAL 5.0 Trouble Shooting OBSERVATIONS The CenSlide 2000 Centrifuge does not appear to have power. ACTIONS Check that the power transformer is securely plugged into both the AC wall outlet and the back of the centrifuge. Try a different AC outlet to verify that AC current is being supplied to the power supply module. Make sure the lid is securely closed and locked in place.
Trouble Shooting Guide OPERATOR’S MANUAL 5.0 Trouble Shooting OBSERVATIONS Sediment appears to pack along the sides of the viewing area. ACTIONS Seat CenSlide tubes firmly and securely within the CenSlide 2000 Centrifuge rotor. If the specimen is extremely thick or turbid, dilute the sample with saline. CenSlide tubes leak. Verify that the caps are firmly and securely in place on the CenSlide tubes. The CenSlide tubes are not made for multiple use.
Trouble Shooting Guide OPERATOR’S MANUAL 5.0 Trouble Shooting OBSERVATIONS ACTIONS The lid to the centrifuge is open Disconnect the power cord at the but the green light is not flashing. back of the unit and then reconnect it. This will cause a power "RESET". The yellow light will not go on when the start button is pressed. Check that there is power to the centrifuge by observing the green light. Check that the lid is securely latched. Microscopic observation and focus difficulty.
After Sale Support OPERATOR’S MANUAL 6.0 StatSpin CenSlide Urinalysis System Warranty StatSpin warrants the CenSlide 2000 Centrifuge and CenSlide tubes to be free from defects in material and workmanship for one year. During the first year, StatSpin will repair or replace your CenSlide 2000 Centrifuge at no charge if it fails to perform as specified.
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