Optical Dissolved Oxygen Meter INSTRUCTION MANUAL HI98198 ® opdo
Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using this meter. This manual will provide you with the necessary information for correct use of this meter, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved.
1. 2. 3. 4. 5. 6. 7. 8. PRELIMINARY EXAMINATION................................................................................................ 4 GENERAL DESCRIPTION........................................................................................................ 5 FUNCTIONAL DESCRIPTION................................................................................................... 6 SPECIFICATIONS.....................................................................................................
PRELIMINARY EXAMINATION 1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com.
The HI98198 opdo® meter is a rugged, portable dedicated dissolved oxygen (DO) meter designed for fresh and saltwater measurements of dissolved oxygen. This professional, waterproof meter complies with IP67 standards and measures DO, barometric pressure, and temperature. The HI98198 opdo meter is only compatible with HI764113 digital dissolved oxygen probe. Concentration measurements are automatically compensated for barometric pressure, temperature and salinity.
FUNCTIONAL DESCRIPTION 3. FUNCTIONAL DESCRIPTION Front View 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.
HI98198 Meter with HI764113 Probe Dissolved Oxygen Barometric Pressure Temperature Range 0.00 to 50.00 mg/L / 0.0 to 500.0% saturation Resolution 0.01 mg/L / 0.1% saturation Accuracy 1.5% of reading ± 0.01mg/L for 0.00-20.00mg/L 5% of reading for 20.00-50.00mg/L 1.5% of reading ±0.1% for 0.0-200.0% 5% of reading for 200.0-500.0% Range 420 to 850 mmHg Resolution 1 mmHg Accuracy ±3 mmHg within ±15% from the calibration point Range -5.0 to 50.0 °C (23 to 122 °F) Resolution 0.1 °C (0.
SPECIFICATIONS 8 HI764113 Probe Probe body material ABS Smart Cap material Polypropylene Cable jacket material PVC Cable length 4 m (13.1 ft.), 10 m (32.8 ft.), and 20 m (65.6 ft.) options Probe guard 316 Stainless Steel Temperature Measurement Thermistor Pressure 20 m (29 PSI) Probe Dimensions (with Guard) 174 X 25 mm (6.8 X 1”) Response Time (t95) 45 seconds Probe Weight (with Guard) 400 g (14.2 oz) 4 m (13.1 ft.
The Hanna HI764113 optical DO sensing probe is based on the principle of fluorescence quenching. The sensing method features an immobilized Pt based luminophore that is excited by the light of a blue LED and emits a red light. Dissolved oxygen quenches this excitation. When there is no oxygen present, the lifetime of the signal is the greatest; as oxygen hits the sensing surface, the lifetime becomes shorter.
PROBE COMPONENTS 6. PROBE COMPONENTS 1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
Install the supplied batteries into the meter. See BATTERY REPLACEMENT for details. To prepare the meter for field measurements close the USB communication socket with the attached stopper. Turn the meter ON by pressing ON/OFF key. At start-up the meter will show the Hanna Instruments logo for a few seconds, followed by the percentage indication of the remaining battery life. Before connecting the probe for the first time, press SETUP and using / arrows navigate to Date/Time.
INITIAL PREPARATION 12 A probe info screen will be displayed momentarily before defaulting to the measurement screen: “No Probe” message is displayed on meter if the probe connector is not plugged in properly. The “No cap info detected. Install the cap and press Continue.“ message on the meter indicates that the Smart Cap is missing or not properly installed on the probe. No conditioning period is required when using a HI764113 DO probe.
Setup menu allows viewing and modifying the measurement parameters. The following table lists the Setup parameters, their valid range and the factory default settings.
SETUP SOUR Configuration Min time Max time Min start DO The minimum time for the SOUR test The maximum time for the SOUR test The minimum DO value for starting the SOUR test Min end DO The minimum DO value at the end of the test Total volume The total volume of the solution to be tested Sample volume The volume of sample in the solution to be tested Solids weight Total solids or Volatile Suspended solids weight SOUR @ 20 °C Correct the SOUR value to 20 °C Autodelete BOD start data Automatically delete BOD
SETUP 8.1. PARAMETER SCREENS Log Separator Option: Comma (,) or Semicolon (;) Log separator or CSV file separator is a special character used to separate columns in the CSV log file. The field separator depends upon regional preferences. Press the function key to modify the CSV file separator. Salinity Option: 0 to 70 PSU Dissolved oxygen solubility decreases if water contains salts. This parameter is used to compensate concentration measurements (mg/L) made in brackish or sea water samples.
SETUP manually enter a pressure value which will be used for oxygen measurements. Once enabled, pressure values are entered from the measurement screen using the / keys. Highlight Manual pressure. Press the displayed functional key to enable or disable the feature. Pressure Unit Option: mmHg, inHg, atm, mbar, psi, kPa The HI98198 has the ability to convert and display pressure measurements in user selected units. Automatic and Manual Pressure will utilize these units.
SETUP Calibration Timeout Option: Disabled, 10, 20, 30,40,50, 60 or 70 days The HI764113 dissolved oxygen probe utilizes optical technology which offers significant advantages over polarographic or galvanic type dissolved oxygen probes. Some advantages include reduced maintenance, simplified handling and infrequent calibrations. However if a user wishes to validate or calibrate on a SOP schedule, a timer may be set as a reminder.
SETUP BOD Configuration When making BOD measurements, the BOD method configuration parameters must be filled in. These parameters will be used to flag error limits for the determination. Ignore this parameter if not making BOD measurements. Highlight BOD configuration. Press Select. Press / keys to modify the selected parameter’s value. Press Prev/Next to select a different parameter. Press Save to save the new BOD configuration. Press ESC to leave without changing.
SETUP Highlight OUR configuration and press Select. Press / keys to modify the selected parameter’s value. Press Prev/Next to select a different parameter. Press Save to save the new OUR configuration. Press ESC to leave without changing. Parameters: • Min time - the minimum time for the OUR test. Range: 1 to 3600 seconds. • Max time - the maximum time for the OUR test. The test will stop automatically when the maximum time has elapsed. Range: 1 to 3600 seconds.
SETUP Highlight SOUR configuration and press Select. Press / keys to modify the selected parameter’s value. Press Prev/Next to select a different parameter. Press Save to save the new SOUR configuration. Press ESC to leave without changing. Parameters: • Min time - the minimum time for the SOUR test. Range: 1 to 3600 seconds. • Max time - the maximum time for the SOUR test. The test will stop automatically when the maximum time has elapsed. Range: 1 to 3600 seconds.
SETUP Autodelete BOD start data Option: Enable or Disable Highlight Autodelete BOD Start Data. Press the displayed functional key to enable/disable the feature. If enabled the BOD initial data record used in BOD result evaluation is deleted automatically after the BOD result has been saved into the meter’s memory (LOG key pressed). If disabled, the user has to delete BOD initial data records that were used in BOD result evaluation, entering View initial BOD data mode.
SETUP Use ←/→ keys to change intensity, then press Accept to confirm. Press ESC to leave without changing. Auto Light OFF Option: 1, 5, 10 or 30 minutes The backlight must be manually enabled by pressing the light bulb key on the keypad. When the selected time has expired, back lighting turns off automatically. To change the duration, highlight Auto Light OFF, then press the desired functional key to change the option.
Use ←/→ keys to select item. Use / SETUP Date / Time This parameter should be configured to the current date and time prior to connecting a HI764113 probe for the first time. To set the time and / or date, from the Setup menu, highlight Date / Time, then press Modify. keys to change the emphasized values. Press Accept to confirm the new settings, or ESC to leave without changing. Time Format Option: AM/PM or 24 hours Your desired time format may be selected in this parameter.
SETUP Highlight desired date format using / Press ESC to leave without changing. keys then press Accept. Language Default language: English This option allows the user to choose the desired language in which all information will be displayed. To modify the language, highlight Language from the Setup menu and press the desired virtual key to make selection. Beep On Option: Enable or Disable This option allows the user to turn an acoustic warning signal on or off.
/ keys to change the meter’s ID. Press Accept to confirm or ESC to exit SETUP Press Modify, then use without saving. Meter Info This parameter captures the firmware version, language version, pressure calibration date, meter serial number as well as the battery capacity. Highlight the Meter Info then press Select. Probe Info This parameter displays probe and Smart Cap information. Press Select to view cap information. Press Next to view the Cap information. Press Prev to return to first screen.
CALIBRATION 9. CALIBRATION There are three calibration functions and procedures that can be accessed through the CAL button. These are calibration of the probe (DO), calibration of the barometer (Pressure) and calibration of the temperature (Temp). Prepare the dissolved oxygen probe according to the instructions from INITIAL PREPARATION section. No conditioning period is required when using a HI764113 DO probe. Store the HI764113 probe in the HI98198 carrying case when not in use.
CALIBRATION For 100% calibration, water-saturated air is the simplest method (and recommended method). It involves suspending the probe over a contained surface of water or moistened absorbent material. The temperature element should also be inside the container.
CALIBRATION Press CFM to confirm the calibration point. Press ESC to leave calibration or continue for a two point calibration. The probe must be rinsed thoroughly with purified water to remove all traces of the zero calibration solution. Single Point Automatic Slope Calibration at 100% Suspend the probe into the water saturated air container and wait for the probe and sample to reach thermal equilibrium. Press CAL. The calibration menu will be displayed. Press DO to select the DO calibration.
Note: If performing the two point calibration, the 0 point must be done first. Submerge the probe into HI7040 zero oxygen solution and stir gently for 2-3 minutes. Press CAL. The calibration menu will be displayed. Wait for the temperature and probe values to become stable. CALIBRATION Two Points Automatic Calibration at 0% Saturation (0 mg/L) and 100% Saturation (8.26 mg/L) Press DO to select the DO calibration. The meter will automatically recognize the 0 standard.
CALIBRATION Single Point Manual Calibration Determine the dissolved oxygen value of the sample using a reference method such as the Winkler titration. Place the HI764113 probe in a sample, lake, basin, stream etc. that a recent dissolved oxygen determination has been made. The probe should be at thermal equilibrium with the sample. Press CAL. The calibration menu will be displayed. Press DO to select the DO calibration. Press the Manual functional key.
CALIBRATION Press CAL from any measure mode (DO, BOD, OUR or SOUR). The calibration menu will be displayed. Press Pressure functional key to select the pressure calibration. The pressure calibration screen will be displayed. Using the / keys, enter the true local barometric pressure reading from the reference barometer. DO NOT use the pressure reported by the weather bureau. Weather bureaus correct pressures to sea level.
CALIBRATION The probe has a built-in temperature sensor and the thermal transfer is made via the stainless steel contact located on the probe body. The contact point must be fully immersed in solution and in thermal equilibrium with the solution measured during calibration. The greater the difference between the temperatures at which the probe was stored and the temperature of the sample, the longer it will take to reach thermal equilibrium.
GLP is a set of functions that allows storage and retrieval of data regarding the calibration of the DO probe. All data regarding DO calibration is stored for the user to review when necessary. Last DO Calibration Data Data from the last 5 DO calibrations are stored automatically after a successful calibration. To view the last calibration data, press GLP when the meter is in the DO, BOD, OUR or SOUR measurement mode. The last calibration will appear at the top of the list.
MEASUREMENT 11. MEASUREMENT Measurements can be made simply and quickly with a calibrated HI764113 and HI98198. The AutoEnd key can freeze data with a single touch, and the measurement units changed by pressing the Range key. Power the opdo® meter with the probe connected. After initialization, the meter enters in measurement mode. Place the probe in the sample to be measured and allow it to reach thermal equilibrium with the sample.
This feature allows the user to log DO, BOD,OUR and SOUR measurements. All logged data can be transfered to a PC using the USB Type C port. Transfer is simple as the computer recognizes the USB connection. A spreadsheet program such as Excel makes integration of the computer with the meter simple. The maximum number of records is 4000. LOGGING 12. LOGGING 12.1. LOGGING THE CURRENT DATA To log the current measurement press LOG key.
LOGGING Press Delete to enter Delete the selected screen. Press CFM to delete record. Press More to view more information of the selected record. Press Pg Down to see additional information. To export data to an USB drive or PC, see the next sections. If there is no logged data, the meter will display “No Records”.
13.1. METER TO PC Logged data on opdo® can be transferred from the meter to a PC by following these simple directions. Suitable operating systems include Windows (XP or newer), OS X or Linux. 1. Connect opdo to the PC using the supplied USB-A to USB-C cable. 2. Turn on opdo. 3. The meter will display “Connected to HOST”. RETRIEVING DATA 13. RETRIEVING DATA The PC should detect the USB as a removable drive. Open the drive to view the stored files. Log files are formatted as Comma separated values (*.
RETRIEVING DATA If the file is already saved on the flash drive, opdo® will ask for confirmation of overwriting the existing file. The message “File exists! OverWrite?”. Press CFM for overwriting the existing file. Note: Do not remove the USB Flash Drive during the file transfer. If the USB is not found the following screen will appear. Try reseating USB drive and pressing Export again.
1. Select measurement units of convenience. Press SETUP to change temperature or pressure measurement units. 2. Press RANGE to access the preferred measurement units; mg/L or % saturation. 3. Routinely Inspect probe for biofouling. Routinely clean off the probe with clean water (between measurements). Biologically active waters may require more frequent cleanings.
BOD MEASUREMENT 15. BOD MEASUREMENT Biochemical oxygen demand (BOD) is an indicator for the concentration of biodegradable organic matter present in a sample of water. It can be used to infer the general quality of the water and its degree of pollution. BOD measures the rate of oxygen uptake by microorganisms in a sample of water at a fixed temperature and over a given period of time.
BOD MEASUREMENT • The type of the Sample: Sample or Seed. • Bottle Volume: the total volume of the BOD bottle. Range: 0.1 to 300.0 mL. • Sample Volume: the volume of sample in the BOD bottle. Range: 0.1 to 300.0 mL (for a seed sample this value is 0.0 mL and cannot be set). • Seed Volume: the volume of seed in the BOD bottle. Range: 0.0 to 300.0 mL. Press Prev/Next to select a different parameter on the screen. Press / keys to modify the selected parameter’s value.
BOD MEASUREMENT Place cleaned and dry probe in sample to be evaluated. Press Compute. The list of the Day 0 samples and seeds will be present. Use the / keys to scroll the list of BOD initial data records. The seed records will have the symbol “*” displayed after the bottle ID. Press More to view detailed information for the selected record. Select the Bottle ID that is currently being measured by the probe and press EvalBOD. The BOD will be calibrated. Press RCL while in the BOD application.
To export to PC Connect cable to PC and meter while in BOD mode. Meter will display “Connected to Host”. A CSV file will appear on PC with BOD data. To export to USB drive While in BOD mode press RCL. Press More. Place the USB into the meter and press Export key. A BOD CSV file will be exported to USB drive. BOD MEASUREMENT Note: “S.C. “ message in the title bar means seed corrected. “not S.C.” message in the title bar means seed not corrected.
BOD MEASUREMENT 44 Note: Various error message will pop up if limits are exceeded. Also if the final DO value is greater than the initial DO value an error message will be displayed.
The OUR is used to determine the oxygen consumption or respiration rate in water. It is defined as the mg/L of oxygen consumed per hour. The following equation is used for OUR determination: where: DOSTART = Dissolved oxygen level at start of test DOEND = Dissolved oxygen level at end of test tELAPSED = Elapsed time of test in seconds total volume/sample volume = Dilution factor of sample Before starting an OUR test remember to set the OUR configuration from the Setup menu.
OUR MEASUREMENT At the end of the analysis the meter will display the computed OUR value, the duration of the measurement, the pressure and the temperature values. Press Log to record a record of this analysis. To analyze another sample, clean and dry off probe. Place probe into the next prepared sample. Press Start. OUR Error messages will be displayed anytime a measurement exceeds the limits that were configured. These will have to be reconfigured and the analysis restarted.
SOUR = OUR / Solids Weight where: OUR is the Oxygen Uptake Rate Solids Weight is the Total solids or the Volatile suspended solids weight in g/L. OUR MEASUREMENT The Specific Oxygen Uptake Rate (SOUR), also known as the oxygen consumption or respiration rate, is defined as the milligram of oxygen consumed per gram of volatile suspended solids (VSS) per hour.
OUR MEASUREMENT During the analysis the meter will display the ongoing calculation. At the end of the analysis the meter will display the computed SOUR value, the duration of the measurement, and the pressure and temperature values. Press Log to record a record of this analysis. To analyze another sample, clean and dry off probe. Place probe into the next prepared sample. Press Start. SOUR Error messages will be displayed anytime a measurement exceeds the limits that were configured.
SOUR recall: OUR MEASUREMENT Press Start to begin a new SOUR test or ESC to return to the SOUR measure screen. Note: In case that the SOUR value was corrected to 20 °C the message “(@20 °C)” will be displayed before the SOUR value.
BATTERY REPLACEMENT 17. BATTERY REPLACEMENT To replace the batteries, follow the next steps: 1. Turn OFF the meter. 2. Open the battery compartment by removing the four screws from the back of the meter. 3. Remove the old batteries. 4. Insert four new 1.5V AA batteries in the battery compartment while paying attention to the correct polarity. 5. Close the battery compartment using the four screws. If the battery capacity is less than 10% the backlight feature is not available.
Cleaning the HI764113 Probe Body • Rinse the probe with clean water to remove debris from around the probe body; wipe with a soft cloth. Unscrew and remove the protective shield and set aside. Ensure weeds, debris, or other materials are not coating the protective guard as this will block sample circulation preventing sample from reaching the active measurement surface.
PROBE MAINTENANCE 1. Unplug the HI764113 from the HI98198 meter. Remove the stainless steel guard from probe body and set aside. Wipe off the probe and cap with a soft tissue. 2. Remove the expired “Smart Cap” from the probe; Squeeze the used cap at the notched V and pull it off the probe body (without twisting). 3. Remove the used o-ring from the o-ring groove by rolling it off the probe. 4. Wipe the o-ring groove with a soft tissue followed by a cleaning with the supplied lens cleaner wipe. 5.
PROBE MAINTENANCE No conditioning period is required when using a HI764113 DO probe. Store the HI764113 probe in the HI98198 carrying case when not in use. For frequent use and short term storage it is recommended to remove the stainless steel guard and replace with the storage vessel that has a small amount of deionized water. The probe can also be stored with the stainless steel guard on in a beaker containing deionized water.
TROUBLESHOOTING GUIDE 54 19. TROUBLESHOOTING GUIDE SYMPTOMS PROBLEM SOLUTION Display shows DO reading blinking. Reading out of range. Recalibrate the meter; Check the sample is within measurable range. Meter shuts off. Dead batteries; Auto Power Off feature is enabled: in this case, meter shuts off after selected period of non use. Replace batteries; Press ON/OFF. The meter does not start when pressing ON/OFF. Initialization error.
Code Description HI7040 Bi-component Zero HI40036P 100 mL plastic beaker (10 pcs.) HI740027P 1.5V AA batteries (12 pcs.) HI764113 Optical DO probe with built-in temperature sensor, shield and 4 m (13’4) cable HI764113-1 Smart Cap with o-ring HI764113-2 Calibration/storage vessel HI764113-3 Stainless steel protective shield HI764113/10 HI764113 with 10 m cable HI764113/20 HI764113 with 20 m cable HI920016 USB Type A to C cable ACCESSORIES 20.
CERTIFICATION CERTIFICATION All Hanna Instruments conform to the CE European Directives. RoHS compliant This device complies with part 15 of the FCC Rules. ID: 2AUGK-HI764133 Disposal of Electrical & Electronic Equipment. The product should not be treated as household waste. Instead hand it over to the appropriate collection point for the recycling of electrical and electronic equipment which will conserve natural resources. Disposal of waste batteries.
WARRANTY FCC Rules, Section 15.105 NOTE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not in-stalled and used in accordance with the instructions, may cause harmful interference to radio communications.
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