About the user manual This user manual is applicable to DNBSEQ-T7RS Genetic Sequencer. The manual version is 3.0 and the software version is V1. This manual and the information contained within are proprietary to Wuhan MGI Tech Co., Ltd. (hereinafter called MGI), and are intended solely for the contractual use of its customer in connection with the use of the product described herein and for no other purpose.
Revision history Date Version May 24, 2022 3.0 February 22, 2021 2.0 December 30, 2020 1.
Contents Safety Conventions 2 General safety 2 Electrical safety 4 FCC statement 4 IC STATEMENT 5 Mechanical safety 6 Components safety 6 Biological safety 6 Symbols 7 Device 7 Label 8 Packaging 9 Device overview Intended use 12 Working principle 12 Structural composition 13 Basic components 15 Front view 15 Keyboard and ports 17 Reagent compartment 18 Flow cell 18 Rear view 19 Ports 20 Control software 21 Self-test interface 21 Main interface 22 Me
Contents Operation area 25 Log interface 25 Settings interface 26 Maintenance interface 27 Shutdown or restart interface 28 About interface 28 Getting started Site requirements 30 Network requirements 30 Operating environments of the computer 30 Software security 31 Data and computer ports 31 Access control 31 Installing the device 31 Powering on or off the device 32 Powering on the device 32 Powering off the device 32 Inspecting the device 33 Preparing th
41 Loading the reagent cartridge 41 Loading the flow cell 41 Reviewing parameters 43 Performing a sequencing run 43 Automatic post-wash 44 Disposing of reagent cartridge and flow cell 44 Maintaining the device Maintaining the main unit 48 Daily maintenance 48 Weekly cleaning 48 Monthly cleaning 49 Maintaining the power supply 49 Maintaining the software 49 Performing a wash 50 Maintaining the pure water container 51 Replacing the waste container 51 Troubleshooti
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Safety This chapter describes basic safety information about the device. Carefully read and understand the information before use to ensure correct operations, best performance, and personnel safety. Keep this manual at hand for reference at any time.
Safety Conventions The following table describes conventions that are used in this manual: Item Description Indicates that the operator should operate the device Warning by following the instructions. Otherwise, it might result in personal injury. Indicates that the operator should operate the device by following the instructions. Otherwise, it might Caution result in device malfunction, damage or inaccurate experiment result.
on Page 47 to ensure best performance. Otherwise, it might result in device malfunction or even personal injury. Caution z Only the technical support authorized by the manufacturer or the qualified and trained personnel can unpack, install, move, and maintain the device. Incorrect operation might cause inaccurate experiment results or damages to the device. z Do not move the device after the technical support have installed and debugged the device.
Safety Electrical safety Warning z Before initial use of the device, assess the electromagnetic environment in which the device will be used. z Do not use the device in close proximity to the source of strong electromagnetic fields, such as unshielded sources of radiated emissions. Radiated signals can reduce the accuracy of the results. z Ensure that the device is properly grounded, and the grounding resistance is less than 4 Ω.
¾ Reorient or relocate the receiving antenna. ¾ Increase the separation between the equipment and receiver. ¾ Connect the equipment into an outlet on a circuit different from that to which the receiver is connected. ¾ Consult the dealer or an experienced radio/TV technician for help. IC statement This device complies with Industry Canada’s licence-exempt RSSs. Operation is subject to the following two conditions: 1. This device may not cause interference; and 2.
Safety Mechanical safety Warning To avoid the device falling off and personal injury, place the device on a level surface that meets the load-bearing requirements and ensure that the device cannot be easily moved. Components safety Warning Only the software that has been provided by the manufacturer can be installed and used on the computer. Unknown software might interfere with normal device functions, or even cause data loss.
Symbols Symbols Device The following table describes symbols on the device: Symbol Name ON "ON" (power) OFF "OFF" (power) General warning sign Description Indicates connection to the mains supply. Indicates disconnection from the mains supply. Signifies a general warning. Warning; biological Warns of a hazard from a biological hazard hazard. Indicates that the marked item can Caution; hot surface be hot and should not be touched without taking care.
Safety Symbol Name Description RFID (Radio RFID frequency Scans the ID of the flow cell or identification) cartridge placed near the area. reader indication Label The following table describes symbols on the label: Symbol Research Use Only Name Indicates a device that is for research / Indicates the medical device manufacturer. Date of Indicates the date when the medical manufacture device was manufactured. Authorized REP use only, and cannot be used for clinical diagnosis.
Name Description Indicates that waste electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be WEEE symbol collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment. SN Indicates the manufacturer's serial Serial number number so that a specific medical device can be identified.
Safety Symbol Name Description Indicates that the transport package Do not roll shall not be rolled or turned over but shall remain in the upright position. Indicates the temperature limits to Temperature limit which the medical device can be safely exposed. Humidity limitation 10 Indicates the range of humidity to which the medical device can be safely exposed. Atmospheric Indicates the range of atmospheric pressure pressure to which the medical device limitation can be safely exposed.
Device overview This chapter describes the intended use, working principle, and structural composition of the device.
Intended use Device overview The device is a sequencing instrument that measures optical and electronic signals of the reporting molecules to decode the sequence information of a piece of DNA or RNA fragment through the use of instrument specific reagents and flow cells imaging hardware, and data analysis software. The device is intended for decoding the sequence of DNAs or RNAs prepared into specific sequencing libraries such as DNA nanoball libraries.
The following figure demonstrates how to load DNBs: Flow cell Decorated sites evenly spread on the flow cell. Each site contains a single DNB. Figure 2 Loading DNBs The DNB and sequencing reagent are pumped into the sequencing flow cell through the device’s liquid system. Each DNB combines with the fluorescence group. The laser excites the fluorescence group to emit light, and the optical signals are acquired by the camera.
Item Device overview Flow cell stage Gas-liquid system E l e c t r i c co n t ro l system Reagent storage system Power supply system Connects the flow cell to fluidics lines and controls the temperature of the flow cell. Provides the gas-liquid support that is required for the biochemical reaction. Controls the electric system. Provides the reagent storage environment. Provides the power supply for the device. Display system Provides the human-computer interaction interface.
Basic components 13 Basic components Front view 13 1 2 3 4 12 11 10 9 8 5 6 7 7 Figure 3 Front view No. 1 2 Name Description T o u c h s c re e n Facilitates on-screen operation and displays monitor information. Flow cell drive button Touch to eject the flow cell drive.
Device overview No. Name 3 Flow cell drive Description Entry of the flow cell. Tap the flow cell drive button to eject the flow cell drive. You can power on or off the IPC, and connect 4 Operation panel USB devices in this area, such as the keyboard and mouse. 5 Supporting feet Supports the main unit to ensure stability. 6 Caster Used for moving the device. Reagent Holds reagent kits and samples at appropriate compartment temperatures. You can press to open the door.
Keyboard and ports 1 Basic components 4 3 2 Figure 4 Keyboard and ports No. 1 Name Keyboard tray latch Description Used to fix the keyboard tray. Used to support the keyboard and mouse. 2 Keyboard tray When not in use, fold the keyboard tray and press it towards the latch until you hear a click. 3 USB 3.0 port 4 Host power button C o n n e c t s t o U S B d e v i ce s s u c h a s t h e keyboard and mouse. Press to power on or off the computer.
Reagent compartment Device overview The reagent compartments include the sequencing cartridge compartments and washing cartridge compartments. The system automatically identifies the QR code of the reagent cartridges by built-in RFID readers. A B C D 1 A B C D 2 Figure 5 Reagent compartment No. Name Description Sequencing 1 Holds the sequencing cartridges at cartridge appropriate temperatures.
No. Name Description Flow cell 1 orientation Shows the direction of placing the flow cell. Basic components marker Rear view 1 2 7 6 5 4 3 Figure 7 Rear view No. Name 1 Handle Description Holds it to easily disassemble the rear panel during maintenance.
Device overview No. Name Description 2 Ventilation outlet Ventilates the device. 3 Power inlet Connects to the power cord. 4 Ports Used for cable connections. 5 6 7 Pure water container port Connects to the pure water container. Optics Used by the technical support to maintain maintenance door the optical system. Maintenance door button Used for opening the door after unlocking. Ports 1 2 3 4 5 6 SBC-LAN BCS-LAN SBC-USB3.0 SBC-USB3.0 BCS-USB3.
No. Name Description Powers on or off the device: 7 Power switch device. z Switch to the OFF position to power off the device. Control software The system control software initiates the communication protocol through physical ports to coordinate with the hardware, control gas lines, fluidics lines, temperature, mechanical components, and optical components.
2. Follow the on-screen instructions or the solutions that are mentioned in Troubleshooting on Page 52. Device overview 3. Perform self-test again: ¾ Tap , select Maintenance > Self-test. ¾ Tap , select Shut down > Restart. If the problem persists, contact the technical support.
Tap on a flow cell stage name and you can enter the main interface of the selected flow cell stage. A B C D Control software Wash Sequence Figure 10 Main interface A Menu area The following table describes the function of menu buttons. Item Description Tap to return to the main interface when the flow cell stage is not preparing for sequencing or washing. Sensor status indicator. Tap to check the status of sensors for all flow cell stages. A red dot appears on the icon when an error occurs.
Item Description After logging into the system, you can tap this button to Device overview lock the screen. The following table describes the function of the sensor status indicators in the menu area. Item Description Connection status with ZLIMS. Disk space status. Device temperature status. The real-time value is displayed on the left. Device humidity status. The real-time value is displayed on the left. Waste level status of the built-in waste container.
Operation area Item Description Flow cell stage name. If an error occurs in a flow cell stage, an error indicator appears on the progress bar. xx% Task progress The flow cell stage is under sequencing. The fluidics lines of the flow cell stage are under washing. The flow cell stage is in idle status. The flow cell stage is preparing for sequencing or washing. Sequencing or washing is being paused. Sequencing or washing is paused. Sequencing or washing is being stopped.
The following table describes the function of controls in the interface: Device overview Item Description Tap to exit the log interface and return to the previous interface. All Tap to view all types of logs. Info Tap to view information-type logs. Warning Tap to view warning-type logs. Error Tap to view error-type logs. Tap to select the date in the pop-up calendar. Flow cell Select the check box to view the logs of a flow cell. Sort by Set the display order of the logs.
Recipe Recipe name Read1 Read2 Barcode DualBarcode Read length Read1 Read2 Dark reaction cycles Save Figure 12 Recipe interface The following table describes the function of controls in the interface: Item Description Tap Recipe to enter the Recipe interface. In this interface, you can: z Tap Sort to change the display order of the recipes. Recipe z Tap Create to customize a recipe. z Tap Import to import recipes. z Tap Export to export customized recipes.
To open the maintenance interface, log in to your account, tap and select Maintenance. , Device overview The following table describes the function of controls in the interface: Item Description Cleaning tool replacement Empty fluidics line Device maintenance Tap to replace the cleaning tool. Tap this button and select a flow cell stage to discharge the residual liquid in its fluidics line to the waste container. Tap to perform a self-test for the hardware of the device.
Getting started This chapter describes laboratory preparations and sequencing preparations.
Site requirements Caution z Ensure that the laboratory floor is level and with a gradient of less than 1/200. z Ensure that the laboratory is free of dust, corrosive and flammable gas, and Getting started heat and wind sources. z Ensure that the laboratory is away from direct sunlight and is well ventilated. We recommend that you refer to the standard of a biosafety level (BSL) 2 laboratory. z We recommend that you use a clean laboratory with ISO Class 8 air cleanliness.
Software environments Warning Only the software that has been provided by the manufacturer can be installed and used on the computer. Because unknown software might interfere with normal device functions, or even cause data loss. If you need to install antivirus software, contact the technical support in advance. z Software architecture: C/S Installing the device z Pre-installed software on the computer includes: ¾ Control software ¾ Microsoft Windows10 64-bit operating system ¾ Microsoft .
T o e n s u re t h a t t h e p e r fo r m a n ce o f t h e d e v i ce m e e t s t h e specifications, the technical support will perform a standard sequencing run before customer training and use. Getting started Powering on or off the device Caution z Ensure that the power switch is in the OFF position before connecting to the power supply. z Ensure that the grounding cable is connected in accordance with the relevant standard or under the guidance of an experienced electrician.
Inspecting the device Caution z Only the technical support of the manufacturer or trained personnel can unpack the device. Contact the technical support to unpack and install the device upon delivery. Failure to do so will void the warranty. z Ensure that the outer package is intact and the indicator status of the anticontact the technical support. The device contains precise components. Therefore, anti-shock and anti-tilt labels are posted on the outer packaging to monitor any effects of transportation.
Preparing the power supply Caution z To ensure a steady and uninterrupted power supply to the device during operation, we recommend the use of a separate UPS. For details about the UPS installation protocol, consult the UPS supplier. Getting started z The required power supply of the UPS should be no less than 5 kVA. Connect the power cord of the device to the output port of the UPS. z Ensure that the voltage meets the requirement to prevent poor performance of electric components.
Preparing samples We recommend that you use the library preparation system of the manufacturer to prepare samples. For details, refer to the relevant user manual. Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run.
