AccuLoader™ Multi-Well Sample Loader . 35℃ 15℃ A-5251 MT Promedt Consulting GmbH Altenhofstr. 80 D-66386 St. Ingbert. Germany.
AccuLoader™ AccuLoader™ Multi-Well Sample Loader User Guide Read all contents in this manual before using the instrument. BIONEER Corporation 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.com Web: www.bioneer.
AccuLoader™ Warranty and Liability All BIONEER products are tested under strict quality control protocols. BIONEER guarantees the quality of all directly manufactured products until the expiration date claimed on the label. If there are any issues in product quality, immediately contact BIONEER Customer Service Center (sales@bioneer.com). BIONEER does not assure liability for use of the product apart from its intended purpose, as described in the appropriate and applicable User Guide.
AccuLoader™ U.S. and Canadian Safety Standards Standard for Electrical Equipment for Measurement, Control and Laboratory Use; ▪ Part1: General Requirements, UL 61010-1:2012, 3rd Ed, 5/11/12, CSA C22.2 No. 61010-1-12 MANUFACTURER : BIONEER CORPORATION 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302 Republic of Korea EUROPEAN REPRESENTATIVE PRODUCT CATALOG NO. : MT Promedt Consulting GmbH Altenhofstr. 80 D-66386 St.
AccuLoader™ FCC ID: 2AYOX-A5251 RF(Radio Frequency): 2402~2480 MHz This device can be operated in at least one Member State without infringing applicable requirements on the use of radio spectrum. FCC Part 15.105 This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
AccuLoader™ Contents I. Introduction ................................................................................................................. 2 1. Principles ................................................................................................................... 2 2. Advantages ................................................................................................................ 2 II. Applications ...............................................................................
AccuLoader™ ............................................................................................................... 7 2. Left side view> ......................................................................................................... 8 3. Right side view> ....................................................................................................... 8 4.
AccuLoader™ I. Introduction AccuLoader™ is a sample dispensing auxiliary instrument used for dispensing a large number of samples into a cartridge for nucleic acid extraction. 1. Principles The contamination shield cover moves automatically so that the opening for sample dispensation corresponds to the well for sample dispensation when a barcode is scanned into the instrument, allowing the operator to dispense the samples into the correct wells. - 2.
AccuLoader™ III. Symbols for Safe Use The symbols used in this manual are as follows. ConformitéEuropéenne Mark Temperature limit Manufacturer Date of Manufacture Authorized representative in the European Community Catalog number The symbols on both the manual and the instrument are as follows. Safety Symbols Do not turn off the application during operation. It may affect the results of the experiment. Do not run any other application during operation.
AccuLoader™ IV. Warnings and Cautions for Safety Read this chapter before using the instrument. The cautions displayed here describe important details on safety and must be followed to prevent accidents and dangers by using the instrument safely and properly. This manual classifies the degree of risks from mishandling into the following three grades.
AccuLoader™ 6) Make sure your hands are dry when plugging or unplugging the power plug. If not, it may cause an electrical shock. 7) Do not place any object that can interfere with the operation near the instrument. 8) Do not disassemble or modify the instrument. It may cause a fire, electrical shock, or failure of the instrument and you will not be able to receive service. 9) Do not give a strong impact or drop the instrument.
AccuLoader™ V. Product Description 1. Composition Components Cat. No. Q’ty A-5251 1 96 well cartridge block - 1 Cartridge block pin - 4 User Guide - 1 Adapter - 1 Power cable - 1 - 1 - 1 AccuLoader™ Spill pad for opening for sample dispensation (consumable) 48-well sample tube rack block – block pin included (optional) Tablet for operation application is not provided by BIONEER. 6 www.bioneer.
AccuLoader™ 2. Structure Name 1 Main Body of AccuLoader™ 2 Contamination shield cover 3 Contamination shield cover handle 4 Opening for sample dispensation 5 Contamination shield cover limit damper 7 www.bioneer.
AccuLoader™ 3. Right side view> No. Name 6 Push power button 7 USB port 8 Barcode reader 9 Power adapter jack 8 www.bioneer.
AccuLoader™ 3. LED status indicator The LED indicator located on the left side of the instrument is as following. RGB LED Colors R e d : Error G r e e n : Ready Y e l lo w : Initializing C y a n : Running P u r p l e : Completed W h i t e : Paused Bluetooth LED Blue: Connected to Bluetooth LED off: Not connected to Bluetooth 9 www.bioneer.
AccuLoader™ 4. Barcode reading 1) Depth of field The depth of field measurements used the following parameters. (1) Distances are measured from the front of the engine. (2) +23℃ (+73℉), 1,600 lux (3) Photographic quality codes Standard Range Typical (in/mm) Symbology Near distance Far distance Delta 4 mil Code 39 4.3/109 5.9/149 1.6/40 5 mil Code 39 3.7/94 7.9/201 4.2/107 7.5 mil Code 39 2.7/68 12.0/305 9.3/237 10 mil Code 39 2.2/55 15.0/381 12.8/326 13 mil 100% UPC 2.0/52 18.
AccuLoader™ VI. Unpacking and Installation 1. Checking the installation environment 1) 2. Check the environmental requirements for installation from “IV. Warnings and Cautions for Safety” Unpacking and installing Pay attention to the details below for unpacking and installation for your safety. ▪ Check the location where you can hold the instrument comfortably. 1) 2) 3) Unpacking (Materials needed: box knife, scissors, or utility knife and gloves, etc.
AccuLoader™ 2) Turn on the instrument by pressing the power button on the left side and check if initialization is performed. 3) Turn on the operation application and start the application. 4) Check the Bluetooth ID on the application and connect to the instrument. 12 www.bioneer.
AccuLoader™ 5) Enter any value in the setting field and tap the Start button 6) Move a sample tube with a barcode to the scanning area of the instrument. The barcodes must be scanned at the exact location for its accuracy. 7) Prepare for experiment if the instrument operates as explained above, or restart both the instrument and the application to check again. 13 www.bioneer.
AccuLoader™ VII. Operation 1. Program description AccuLoader program is an application operating program. The intention of AccuLoader™ operation is to assist operators in dispensation of samples with accuracy, especially for pooled samples. 2. Running the program 1) Turn on the instrument and run AccuLoader application after initialization. 14 www.bioneer.
AccuLoader™ 2) Home screen will appear after the first splash screen disappears. It will display the previously set conditions. 3) Tap the SETUP button on the left side of the screen. The ID address of Bluetooth differs for each instrument. 4) Select AL_BNR. 15 www.bioneer.
AccuLoader™ 5) Assign Sample Count and Pooling Count. Description Sample Count Total number of samples including the controls Pooling Count Number of samples per well excluding the controls Sample Volume Has initial value of 400 µl Pooling Volume Automatically calculated from the assigned sample count and pooling count values 16 www.bioneer.
AccuLoader™ 6) Tap the START button on the lower right side of the screen when all settings are complete. 7) Run screen will appear. An animation will display the location for the NTC (no template control) well. Scan the NTC tube with the barcode scanner and the plate will move from the starting position so that the opening is on the NTC well. Open the NTC tube cap and dispense the assigned volume of NTC into the NTC well using a pipette. 17 www.
AccuLoader™ 8) An animation will display the location for the PC (positive control) well. Scan the PC tube with the barcode scanner and the plate will move so that the opening is on the PC well. Open the PC tube cap and dispense the assigned volume of PC into the PC well using a pipette. 18 www.bioneer.
AccuLoader™ 9) Scan the first sample tube with the barcode scanner and dispense the assigned volume of samples into the designated wells using a pipette. Repeat the barcode scanning and sample dispensation steps as many times as the assigned pool size for the sample tubes. 10) All completed status will be displayed. When all dispensation has been completed, “Remain Tube: 0” and “Load Completed!” will be displayed at the bottom and the upper right side, respectively.
AccuLoader™ 11) In case of dispensing into a new cartridge, repeat from Step 5. 20 www.bioneer.
AccuLoader™ VIII. Maintenance 1. General maintenance 1) 2) 3) 4) 5) 6) Do not spill liquid or ignore contamination on the instrument for stable daily experiments. Do not use abrasive detergents. It may damage the surface of the instrument. Clean the instrument after the experiment. Use a lint-free cloth dampened with mild, neutral detergent. Do not use highly acidic or basic detergent. Wipe it immediately if it gets on the instrument. Decontaminate the instrument first when transferring the instrument.
AccuLoader™ 6. Power adapter Use a Safety Standard certified adapter with a capability of or higher for the instrument. The adapter connector must have an outer diameter of 5.5 mm and an inner diameter of between 2.1 and 2.5 mm. 7. Storage and handling Storage conditions 1) Temp: 0 - 40℃ 2) Humidity: 20 - 80% (No condensation) Storage of the instrument 1) Store the instrument following the warnings and precautions provided in the manual when not in use.
AccuLoader™ IX. Troubleshooting If the instrument does not operate properly, check the following and contact your local distributor if not resolved. Problem Action The instrument does not turn on. 1. Check the connection between the adapter and the electrical outlet. 2. Check the connection between the adapter and the power cable. 3. Check the connection between the adapter connector and the adapter jack at the back of the instrument. 4. Check if the power button is at the off position. 5.
AccuLoader™ X. Technical Specifications General specifications Dimensions Weight Power 34.1 cm (W) x 27.2 cm (D) x 11.8 cm (H) 13.43 in (W) x 10.71 in (D) x 4.65 in (H) 4.5 kg (9.93 lbs) Adapter input: 100-240 V~, 50/60Hz, 1.5A Max Output: 24V DC, 2.5A Instrument Input: 24V DC, 2.5A Operation specifications Temperature 15 – 35°C (59.0 – 95.0℉) Humidity 20 - 80%, no condensation System Operation control via a mobile application Communication Bluetooth Receipt of info.
AccuLoader™ XI. Technical Support 1. Requesting for service Fill out the “Service Request Form” to request for service. 2. Shipping the instrument for service 1) Inform your local distributor of the details of the service request. 2) Decontaminate the instrument. Decontaminate the instrument using a decontamination solution if it is contaminated by biohazardous materials. If you cannot remove the contaminant, you may not receive the service.
AccuLoader™ XII. Warranty All BIONEER instruments are tested under strict quality control protocols. 1. Warranty period ▪ Instrument: 1 year ▪ Accessories and consumables: 3 months ▪ Retention period of spare parts: 5 years from the discontinuation date of the model 2. Warranty details ▪ BIONEER provides free repair service for defects that occurred while following the provided instructions within the warranty period. ▪ All parts other than the main body are consumables.
Appendix B Certificate of Decontamination Decontamination and disinfection procedures of the instrument must be performed for repair or return before shipping. This must be performed for safety of both the customer and the manufacturer. Instrument: Serial no.
Appendix C Service Request Form AccuLoader™, Multi-Well Sample Loader Product Catalog No. A-5251 Serial No.
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8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea +82-42-930-8777 (Korea: 1588-9788) +82-42-930-8688 sales@bioneer.com www.bioneer.com 155 Filbert St. Suite 216 Oakland, CA 94607, USA +1-877-264-4300 (Toll-free) +1-510-865-0350 order.usa@bioneer.com us.bioneer.com Korea Bio Park Bldg. #B-702, 700 Daewangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13488, Republic of Korea +82-31-628-0500 +82-31-628-0555 sales@bioneer.co.kr www.bioneer.co.
