Ultra-Portable Real-Time Fluorescence Quantitative PCR Instrument Maverick qPCR Instruction Manual Version（V2.1） The instruction manual must be properly placed in the product box during shipment. The user is required to keep this manual in a safe place so that it can be consulted when needed. All rights reserved. All information contained in this manual is subject to change without notice. Manufacturer: Anitoa Biotechnology (Hangzhou) Co., Ltd.
Anitoa warrants that the MQ4 Fluorescence PCR instrument you are using has been fully tested and meets the requirements of the instructions for use. The instructions and safety warnings given in this instruction manual must be followed in order to use the instrument, otherwise the warranty does not apply. Software Description： The software is a necessary tool for the operation of the instrument and Anitoa has the right to modify its functions or design, etc.
Anitoa Biotechnology (Hangzhou) Co., Ltd. CONTENTS Chapter 1 Important Notes ......................................................... 1 1.1 Instrument Grounding .......................................................... 1 1.2 Placement of the Instrument ................................................... 1 1.3 Operation Note ................................................................ 2 1.4 After-Sales Service ........................................................... 2 1.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 1 Important Notes The following basic safety measures must be observed during all phases of operation, maintenance and repair of this instrument. Failure to observe these measures or the warnings and precautions indicated in this manual will likely affect the basic protection provided by the instrument. It will also undermine the safety standards for which the instrument was designed and manufactured, as well as the intended scope of use of the instrument.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 2) The instrument uses semiconductor cooling and fan-assisted heat dissipation, so when placing the instrument, ensure that there are no obstacles within 15 cm of the instrument. 3) The instrument should be placed in a place with low humidity, less dust and far from water sources (such as pools, water pipes, etc.), with good ventilation, no corrosive gases or strong magnetic fields, and avoid direct sunlight and strong light sources.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.5 Packaging, Storage and Transportation Marking Table 1.5 Package storage and transportation identification Symbol Description Title Place carefully and gently Position This symbol is used to indicate that the product is a precision instrument and should be handled On the packing carton carefully and gently.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.6 Instrument Identification Information Table 1.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.7 FCC Statement This device complies with part 15 of the FCC rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 2 Product Overview This chapter mainly describes the usage, characteristics, specifications and performance parameters of MQ4 fluorescence quantitative PCR instrument. 2.1 Product Usage The MQ4 model is an ultra-portable qPCR instrument with a large 10" touch screen for integrated operation. The product is developed based on our multi-channel fluorescence imaging optical system with CMOS bio-image sensor.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 2.3 Specification Model Description MQ 4 16 4 4：Represents the number of channels of the instrument 16：Represents sample throughputs 4： Represents appearance number MQ：Represents series number 2.4 Instrument Models Table 2.4 Instrument models Channel Sample throughput Model MQ4044 4 channels 4 wells MQ4162 2 channels 16 wells MQ4164 4 channels 16 wells 2.5 Main Technical Parameters Table 2.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Fluorescence Detection System Performance Light Source High Brightness LED Detector Ultra-Low-Light CMOS Image Sensor (CIS) Chips Excitation Wavelength Detection Wavelength F1：470nm F2：523nm F3：571nm F4：624nm F1：527nm F2：564nm F3：612nm F4：694nm 2.6 WIFI and BT The XY8788 module provides a shared antenna interface ANT_WIFI/BT for WIFI and BT with an impedance of 50Ω .
Anitoa Biotechnology (Hangzhou) Co., Ltd. 2.6.2 WIFI performance indicators The following table lists the transmit and receive performance of XY8788 WIFI Table 24: Transmission performance of WIFI Output Power standard rate 2.4G Hz 5GH z 802.11b 1Mbps 17±2dBm 802.11b 11Mbps 16±2dBm 802.11g 6Mbps 15±2dBm 802.11g 54Mbps 15±2dBm 802.11n HT20 MCS0 14±2dBm 802.11n HT20 MCS7 13±2dBm 802.11n HT40 MCS0 10±2dBm 802.11n HT40 MCS7 10±2dBm 802.11a 6Mbps 10±2dBm 802.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Reference Specification serial document number numb er IEEE 802.11n WLAN MAC and PHY, October 2009 + IEEE 802.11-2007 WLAN MAC and PHY 1 PHY, June 2007 IEEE Std 802.11b, IEEE Std 802.11d, IEEE Std 802.11e, IEEE Std 2 802.11g, IEEE Std 802.11i: IEEE 802.11-2007 WLAN MAC and PHY, June 2007 2.6.3 BT overview XY8788 module supports BT v2.1+EDR, 3.0+HS, v4.1+HS, V4.2 . The modulation mode supports GFSK, 8PSK, π/4QPSK. Supports up to 7 wireless connections.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 3 Instrument Installation This chapter describes the use and storage conditions of the MQ4 ultra-portable quantitative fluorescence PCR instrument, its construction, removal of the fixture, installation/uninstallation of the software, and preparation for power-up. 3.1 Environmental Conditions 1) Transportation and storage conditions of the instruments a. Environmental temperature：－20℃～55℃ ； b. Relative humidity：≤85％ 2) Working conditions requirements a.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Power Adapter 1 USB Cable 1 Instruction Manual 1 Factory Inspection 1 Report Warranty Card 1 Certificate of 1 Conformity 3.4 Power Cord Connection 1) Connection of adapter: the adapter supplied with the instrument should be used to connect the adapter to the instrument. 2) Connection of power cord: the power cord supplied with the instrument should be used.
Anitoa Biotechnology (Hangzhou) Co., Ltd. SampleTable Location Instrument Running Indicator Light Figure 3.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 4 Software Operation Guide Anitoa qPCR software can be used to set up experiments, run experiments, collect data, analyze and manage experiments. The software contains four main functional modules, namely "User", "Test", "Results", and "System". 1) "User" module: mainly contains upload configuration, user management, image, and manufacturer settings.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 2) When asked if the USB connection is allowed, click < OK>, as shown in Figure 4.1-2 Figure 4.1-2 USB Access interface 4.2 Experimental Setup 1) In the test interface, click "New Experiment" (Figure 4.2-1) to enter the experiment settings 1 Figure 4.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 2) In Experiment Setup 1 (Figure 4.2-2), enter the experiment name, select the corresponding channel, select the well position, and enter the sample information, etc., and click "Next" to enter Experiment Setup 2. Figure 4.2-2 Experimental setup 1 3) In Experimental Setup 2 (Figure 4.2-3), set the reaction program (parameters such as reaction temperature, reaction time, number of cycles, photo stage, etc.) Figure 4.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 4.3 Save Template 1) Click the "Save Template" icon in the upper right corner to save the current template. Figure 4.3-1 Program setting interface 2) The saved templates will appear in the "Test" screen for selection. Click "Import Template" in the upper right corner to import other experiment templates. Figure 4.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 4.4 Running Experiments After the experiment setting is completed, click the "Start" button to start the experiment and enter the "Run" interface. Click "Force Stop" at the bottom right corner to stop the experiment. Figure 4.4-1 Running interface 4.5 Experimental analysis 1) Click the "Results" button on the main page to enter the "Data List" page, as shown in Figure 4.5-1.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.5-1 Data list page 2) To view the amplification curve: click on the experimental data and enter the "Result Analysis" page, as shown in Figure 4.5-2. Figure 4.5-2 Result analysis interface 3) Experimental analysis a. Adjust the baseline Ct lower limit. b. Adjust Ct threshold percentage: default is 10% . c. Normalization: Normalization of the amplification curve does not affect the results.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.5-3 Experimental analysis interface d. Click the "Filter" button in the upper right corner to enter the page in Figure 4.5-4 and select the well and channel for data analysis. e. Click the "Result" button in the upper-right corner to return to the Figure 4.5-3 page. Figure 4.4-5 Data filtering screen 4) View standard curve a.
Anitoa Biotechnology (Hangzhou) Co., Ltd. interface (see Figure 4.5-4). b. Manually enter the standard concentration, plot the standard curve, and calculate the concentration of the unknown sample based on the Ct value. Figure 4.5-4 Standard curve interface 4.6 Data Export 1) Click the "Export" button on the experiment analysis interface to export the relevant information of the experiment. Currently, there are four export formats: PDF, CSV, JSON and JPG.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.6-1 Data Export 2) Select one of the export formats, such as "PDF" to enter the PDF preview interface. Figure 4.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.6-3 Whether to print the confirmation page 4) Click the "OK" button to enter the Figure 4.6-4 page Figure 4.6-4 Save as PDF file page 5) Click the upper left corner of the "drop-down" button, "Save as PDF" or "Add Printer" option, as shown in Figure 4.6-5 (Note: The printer needs to support Android system) Figure 4.
Anitoa Biotechnology (Hangzhou) Co., Ltd. 6) Click the "Save as PDF" option to save the file in the system directory, the storage path: /sdcard/anitoa/report Figure 4.6-6 Save path 7) System save default path： a. PDF path：/sdcard/anitoa/report b. CSV path：/sdcard/anitoa/report c. JSON path：/sdcard/anitoa/imageData d.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 5 Instrument Maintenance 5.1 Instrument Cleaning (1) Instrument surface cleaning: The surface of the instrument should be scrubbed regularly with a soft cloth with 75% alcohol, and the instrument should be wiped dry after cleaning. (2) Reaction wells cleaning： a. It is recommended that the wells be cleaned once every 3 months as dust or impurities can affect PCR amplification and fluorescence detection. b.
Anitoa Biotechnology (Hangzhou) Co., Ltd. force all ongoing operations to stop. (2) After the above-mentioned failure of the heating system, the user should stop using the instrument and contact the manufacturer for repair in time. 5.5 Operation Requirements (1) During the use of the instrument, the operator may be exposed to residual substances harmful to organisms or infectious substances, and the operator needs to be trained and have relevant qualifications.
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 6 FAQ No.
Anitoa Biotechnology (Hangzhou) Co., Ltd.
