50 Series User’s Manual 1
150 Series Table of Contents Before Use.......................................................................................... 3 Introduction ............................................................................................................. 3 Safety Precautions................................................................................................... 4 Parts .............................................................................................. 5 M150 ........................
150 Series Before Use Introduction Congratulations on the purchase of your new AmScope microscope! This manual is designed for the M150 series microscopes. Please be sure to take a few minutes to familiarize yourself with the features and functions of your new AmScope microscope. If you’d like more information on microscopes, parts, or accessories, please visit our website at: www.iScopeCorp.
150 Series Before Use Safety Precautions 1. As the microscope is a precision instrument, always handle it with care, avoiding impact or abrupt movement during transportation. Do not shake the package. 2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in maximum relative humidity of 85%. 3.
150 Series Parts M150 Eyepiece Ocular Tube Rotating Monocular Head Nosepiece Turret Objectives Limit Stop Stage Clips Stage Disc Diaphragm (Disc Iris) Fine Focusing Knob Coarse Focusing Knob Illuminator Lens 5
150 Series Parts Definition of Parts Base Illuminator Lens Directs the light source towards the slide Focusing Knob Used to bring the slide into the sight and focus Disc Iris Diaphragm Controls the amount of light that hits the slide from the base illuminator Dimmer Controls the amount of light that escapes from the base lens Limit Stop Knob Limits the upward movement of the mechanical stage in order to avoid damaging the slide and objective Nosepiece Houses the objective lenses Rotating Monocular Head Ho
150 Series Getting Started Assembly 1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the optical items. Check carefully to ensure that all parts and accessories are intact. 2.
150 Series Operation Setting Up 1. Place the specimen to be studied on a glass slide (or use a prepared slide). Place it on the stage, holding it snugly in place with the metal slide holders (clips) of the mechanical stage. 2. Center the specimen over the stage opening, lining it up with the light and the objective lens. 3. To adjust the illumination, slowly turn the dimmer on the right side of the base until the desired intensity of light is achieved. Focusing 1.
150 Series Operation Setting the Stage’s Stop-Limit 1. To adjust the stop limit on the stage, unlock it by unscrewing the lower nut. The stop limit is located at the rear of the stage. 2. Once tension is released on the nut by rotating it counter clockwise, you may adjust the upper thumb screw to move the limit up or down as desired. 3. Lock the limit stop nut back into place after the desired stage height is achieved.
150 Series Operation Maintenance/Precautions - All glass surfaces must be kept clean. Fine dust on the optical surface should be blown off using a can of compressed air or gently wiped off with a soft lens paper tissue/nonabrasive lint free cloth. - Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens cleaner (we recommend Sparkle brand optical cleaner). - Do not use Sparkle to clean other elements of the microscope.
150 Series Specifications 150 Series Specifications Parts Specifications WF Eyepiece WF10X/18mm M149 WF10X/18mm w/ Pointer M149A x M149B M149C x x x WF10X/18mm w/ Reticle WF16X/18mm x WF20X/18mm x WF25X18mm Plan Eyepiece x P5X P10X P16X 4X/0.10 x x x x 10X/0.25 x x x x 40X(spring)/0.65 x x x x Monocular, 360 Degree Swiveling x x x x Diaphragm Iris Diaphragm Disc x x x x Illumination Halogen Light w/ Dimmer x x x x DIN Achromatic Objectives 60X(spring)/0.
150 Series Specifications Optional Accessories Parts Description Eyepiece 5X EP5X23 Obtaining 20x, 50x, 200x, and 500x magnification powers 20x EP20X23 Obtaining 80x, 200x, 800x, and 2000x magnification powers 25x EP25X23 For obtaining 250x and 2500x magnification powers 10x w/ Pointer EP10X23P For easier identifying of objects 10x w/ Reticle Objective Camera Purpose EP10X23R For measuring objects 2X A2X For obtaining 20x and 32x magnification powers 5X A5X For obtaining 50X and 8
150 Series Specifications Objectives Type DIN Achromatic Objective (195mm) Plan Objective (195mm) Magnification Numerical Aperture (N.A.
150 Series Technical Parameters Electrical System There are two options for electrical systems for this series of microscope. The light source is an LED system. 1. 220V~240V power supply: 220V~240V ±10%, 50Hz This electrical system is CE and GS certified 2. 100V~120V power supply: 100V~120V ±10%, 60Hz This electrical system is UL certified. All units come standard as 110V units unless an upgrade to a 220V system is requested. Upgrade fee is dependent on which unit is purchased.
0 Series Technical Parameters Technical Terms & Concepts Total Magnification Total magnification of a microscope is calculated by the magnification of the objective multiplied by the magnification of the eyepieces. -Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification Field of View Linear field of view of the eyepiece divided by the magnification of the objective Numerical Aperture (N.A) Calculated by n Sin α (max), the Numerical Aperture (N.
150 Series Troubleshooting Common Issues Symptom Cause Remedy OPTICAL ISSUES One side of the field of view is darker The nosepiece if misaligned Turn the nosepiece until it clicks into place Stains or dust has accumulated on the objective, eyepieces, or base lens Clean all lenses with lens cleaner or a lint free nonabrasive cloth Obstructions are observed in the field of view Stains, dust, or dirt has accumulated on the specimen Clean the slide or use a new specimen if sample is destroyed Stain
150 Series Troubleshooting Common Issues (Continued) Symptom Cause Remedy OPTICAL ISSUES The color of the image is not accurate The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color clarity No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired filter MECHANICAL ISSUES The objective touches the cover slip The cover slip is not standard sized Replace the cover slip with the ap