20 Series User’s Manual 1
120 Series Table of Contents Before Use.......................................................................................... 3 Introduction ............................................................................................................. 3 Safety Precautions................................................................................................... 4 Parts .............................................................................................. 5 B120 ........................
120 Series Before Use Introduction Congratulations on the purchase of your new AmScope microscope! This manual is designed for the B120 series microscope. Please take a few minutes to familiarize yourself with the features and functions of your new microscope. If you’d like more information on microscopes, parts, or accessories, please visit our website at: www.iScopeCorp.
120 Series Before Use Safety Precautions 1. As the microscope is a precision instrument, always handle it with care, avoiding impact or abrupt movement during transportation. Do not shake the package. 2. Do not place the microscope in direct sunlight or in high heat. Keep it indoors in a dry and clean place with temperatures between 32-100 degrees F (0-40 degrees C), and in maximum relative humidity of 85%. 3.
120 Series Parts B120 Binocular Siedentopf Head Eyepieces Nosepiece Turret Objectives Limit Stop Mechanical Stage Coarse Focusing Fine Focusing Condenser Iris Control Mechanical Stage Controls Base Lens Dimmer 5
120 Series Parts T120 Trinocular C-Mount Port Eyepieces Nosepiece Turret Objectives Limit Stop Mechanical Stage Coarse Focusing Fine Focusing Condenser Iris Control Mechanical Stage Controls Base Lens Dimmer 6
120 Series Parts Base & Condenser 7
120 Series Parts Definition of Parts Base Lens Directs the light source towards the slide Coarse Focusing Knob Used to initially bring the slide into the sight and focus Condenser & Iris Diaphragm Controls the amount of light that hits the slide Condenser Screw Screws the condenser in place, securing it to the mechanical stage Dimmer Controls the amount of light that escapes from the base lens Diopter Allows the focus to be perfected for both eyes, independent of each other Fine Focusing Knob Used to refi
120 Series Getting Started Assembly 1. First, take the styrofoam container out of the cardboard carton and lay it on its side, paying attention to which side is labeled up. Remove the tape and open the container carefully so as to avoid dropping and damaging the optical items. Check carefully to ensure that all parts and accessories are intact. 2.
120 Series Operation Setting Up 1. Adjust the microscope head so that it is in a comfortable position for observation. 2. With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyeubes and rotating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both eyes. (mounted on the Siedentopf head). 3. Place the specimen to be studied on a glass slide (or use a prepared slide).
120 Series Operation Adjusting the Condenser & Diaphragm 1. Using the condenser-adjustment knob (left of unit, under the stage), you can change the distance between the light condenser and the stage. This allows you to control the concentration of the light hitting your slide. 2. By changing the aperture (hole size) of the iris diaphragm, you can adjust the background brightness. Adjust the aperture of the iris diaphragm using the iris adjustment slider located directly under the stage. 3.
0 Series Operation Adjusting Focusing Tension 1. To adjust the tension of the focusing knobs, first locate the black ridged tension knob on the inside of the coarse focusing knob. 2. To decrease tension, rotate the adjustment forward, towards the stage (counterclockwise). To increase, rotate away from the stage (clockwise). Note: If your stage is slipping down after setting the focus, you need to increase the tension. Maintenance/Precautions - All glass surfaces must be kept clean.
120 Series Specifications B120 Series Specifications Parts Specifications B120A B120B B120C x x x WF10X/18mm WF10X/18mm w/ Pointer WF Eyepiece WF10X/18mm w/ Reticle WF16X/13mm x WF20X/10mm x WF25X x P5X Plan Eyepiece P10X P16X 4X/0.10 x x x 10X/0.25 x x x 40X(spring)/0.65 x x x x x x x x x Coaxial Focusing System x x x Quadruple Nosepiece x x x 128mm x 127mm Mechanical Stage x x x Movement Range: 70mm x 30mm x x x Condenser Abbe, NA= 1.
120 Series Specifications T120 Series Specifications Parts Specifications T120A T120B T120C x x x WF10X/18mm WF10X/18mm w/ Pointer WF Eyepiece WF10X/18mm w/ Reticle WF16X/13mm x WF20X/10mm x WF25X x P5X Plan Eyepiece P10X P16X 4X/0.10 x x x 10X/0.25 x x x 40X(spring)/0.
120 Series Specifications Optional Accessories Parts Description Eyepiece 5X EP5X23 For obtaining 20x, 50x, 200x, and 500x magnification powers 20x EP20X23 For obtaining 80x, 200x, 800x, and 2000x magnification powers 25x EP25X23 For obtaining 250x and 2500x magnification powers 10x w/ Pointer EP10X23P For easier identifying of objects 10x w/ Reticle Objective Darkfield Condenser EP10X23R For measuring objects A2X For obtaining 20x and 32x magnification powers 5X A5X For obtaining 5
120 Series Specifications Objectives Type DIN Achromatic Objective (195mm) Plan Objective (195mm) Magnification Numerical Aperture (N.A.
120 Series Technical Parameters Electrical System There are two options for electrical systems for this series of microscope. The light source is dependent on which model you have, but can either be a 6V/20W halogen, a 6V/30W halogen, or an LED system. 1. 220V~240V power supply: 220V~240V ±10%, 50Hz This electrical system is CE and GS certified 2. 100V~120V power supply: 100V~120V ±10%, 60Hz This electrical system is UL certified.
120 Series Technical Parameters Technical Terms & Concepts Total Magnification Total magnification of a microscope is calculated by the magnification of the objective multiplied by the magnification of the eyepieces. -Ex: (10x Eyepieces) x (4x Objective) = 40x Total Magnification Field of View Linear field of view of the eyepiece divided by the magnification of the objective Numerical Aperture (N.A) Calculated by n Sin α (max), the Numerical Aperture (N.
120 Series Troubleshooting Common Issues Symptom Cause Remedy OPTICAL ISSUES One side of the field of view is darker The nosepiece if misaligned Turn the nosepiece until it clicks into place Stains or dust has accumulated on the condenser, objective, eyepieces, or base lens Clean all lenses with lens cleaner or a lint free nonabrasive cloth Obstructions are observed in the field of view Stains, dust, or dirt has accumulated on the specimen Clean the slide or use a new specimen if sample is destr
120 Series Troubleshooting Common Issues (Continued) Symptom Cause Remedy OPTICAL ISSUES The brightness adjustment knob is not in the right position Adjust the brightness knob to a higher or lower setting for color clarity No filter is used or filter is in use Remove color filter if natural light is desired, or insert desired filter (Trinocular Only) I cannot see anything in the camera / Image is black The trinocular port shutter of the microscope is not open Slide the silver metal pin out on the
