Allegro 3D, Inc.
Contents 1. Safety and compliance ...............................................................................................................................2 1.1 General safety information ................................................................................................................. 2 1.2 Protective equipment ..........................................................................................................................3 1.3 Opening the product and service .................
1. Safety and compliance Before you start using your BIONOVA X Model NOVAX-1001, please carefully review the entire manual. Improper operations of the bioprinter can lead to serious personal injury and equipment damage. 1.1 General safety information Keep your workplace tidy. Do not operate the BIONOVA X Model NOVAX-1001 in the presence of flammable liquids, gases, or dust.
1.2 Protective equipment WARNING! ● Always wear protective goggles and gloves, and make sure the environment around the instrument is secure from other personnel while operating the printer. ● Always wear protective goggles and gloves while handling dangerous materials that can be absorbed by the skin or which are toxic, strike-attractive, corrosive, allergy inducing, carcinogenic, reproduction-endangering, or mutagenic to humans.
2. Specifications 2.1. BIONOVA XTM Model D Diagram A. Sliding door B. Printing probe C. Well plate tray D. Power supply E. Adjustable legs F. USB port G. Power button H. Recessed handle I.
2.2. Technical Specifications Bioprinting technology Dimensions Digital light projection based stereolithography system 20.3” (W) x 15” (D) x 17.4” (H) 515 mm (W) x 380 mm (D) x 441 mm (H) Weight 90 lbs (41 kg) Resolution 10 μm LED wavelength 405 nm Heater temperature Room temperature to 60 °C Well plate format 24 well plate, 12 well plate, 6 well plate Support file types .stl, .png, .bmp, .
3. Getting started with BIONOVA X 3.1. Unpacking and installation 1. Carefully open the crate and remove the printer from the packaging by grasping the printer firmly from the bottom with two people. 2. Set the printer on a leveled and steady table or biosafety cabinet surface. 3. Use the power cord to connect the printer to a power supply via the port located on the left side of the printer. 4. Power on the printer by pushing the power supply switch located on the side of the printer. 5.
4. Your first bioprint with BIONOVA X 4.1. Setting up BIONOVA X 1.. Power on the printer by pushing the power supply button located on the side of the printer. 2. Power on the computer by pushing the power button located in the front of the printer. The printer will initialize the software and the position of the well-plate tray. After initialization, the software interface will load the blank Project page as shown below. 3. Make sure the sliding door is closed. 4.
4.2. Loading bioprinting probe 1. Once the UV sterilization is completed, open the sliding door. 2. Remove the provided printing probe from the sterile packaging in a biosafety cabinet. Caution: choose the right printing probe (i.e., 6, 12, or 24-well probe) to match the well plate (i.e., 6, 12, or 24-well plate) to be used for printing. 3. Carefully retrieve the probe adapter from above the well-plate tray.
4. Press the Load Position button on the Utility page and carefully load the well plate onto the tray after it is moved to the load position. Caution: please make sure the well plate is placed in the right orientation as shown on the Project page and secure the well plate in the tray using the clamp. Printing probe and well 6-well probe and 6- 12-well probe and 12- 24-well probe and 24- plate used well plate well plate well plate Maximum volume of bioink for each well (ml) 8.6 3.4 1.3 4.4.
5. Go to the Well Plate page and choose the desired well plate type from the drop-down menu. 6. Well groups can be added or deleted. Wells can be assigned to or removed from each well group by selecting or deselecting the wells when the target well group is highlighted on the right panel.
7. 3D models (.stl files) or 2D image files (.png, .jpg, .bmp files) can be loaded for each well group on the 3D Model page. When loading 3D models, the software will ask for the maximum dimensions in x, y, z directions and the number of layers to be sliced. When loading 2D image files, the software will only ask for the dimension in z direction as the x, y dimensions are set by the 2D image design.
Printing probe and 6-well probe and 6- 12-well probe and 12- 24-well probe and 24- well plate used well plate well plate well plate Maximum dimension in X direction (mm) Maximum dimension in Y direction (mm) Maximum dimension in Z direction (mm) 19.2 9 7 10.8 9 7 9 9 6.5 8. For each 3D model or 2D image loaded for each well group, you can set one single section or multiple sections with variable printing parameters (i.e., light intensity, motion speed, section height) in the Sections page.
9. Once all the parameters are set, you can choose to print the complete project or only selected wells in the Print page. Start printing by pushing the Print button. The finished wells will be labeled as green, the ongoing well will be labeled as red, and the selected wells to be printed are labeled as grey. 10. You can pause or stop the printing at any time during the printing. 11. Once the printing is stopped or completed, the well plate tray will automatically return to the load position.
4.5. Retrieving the bioprinted samples 1. Please make sure the tray is in the load position before retrieving the well plate or the printing probe. 2. Open the sliding door and carefully remove the well plate from the tray. The bioprinted samples are ready for culturing, imaging or assaying. 3. Carefully retrieve the printing probe without touching the well plate tray. Clean and store the printing probe in a sterile container for later use. 4. Close the sliding door. 4.6. Powering off the printer 1.
5. Consumables 5.1. Bioprinting probes Our probes are specially designed for our BIONOVA X 3 D printer to print directly into multi-well plates (24, 12, and 6-well plate). The probe tips are specially coated to prevent adhesion of 3D printed scaffolds to the probe during printing. Our probes are available in three tip sizes, small, medium and large, which are designed specifically to work with our 24-well plate, 12-well plate and 6-well plate.
5.2. Multi-well plates Our multi-well plates (24, 12, and 6-well) are compatible with our BIONOVA X 3 D printer and printing probes. Our multi-well plates with high performance #1.5 cover glass (~0.170 mm) are suitable for high resolution imaging systems. The glass bottom is specially coated to enhance adhesion of 3D printed tissues and avoid tissue detachment during medium change.
● Choose the appropriate bioprinting probe for different multi-well plate. For example, use the probe with small tip for the 24-well plate, the probe with medium tip for the 12-well plate, and the probe with large tip for the 6-well plate. ● The new multi-well plate is sterile and comes in a sterile plastic bag. The new multi-well plates can be used directly for bioprinting.
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